1.1.1.28: D-lactate dehydrogenase

This is an abbreviated version, for detailed information about D-lactate dehydrogenase, go to the full flat file.

Reaction

(R)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

D-(-)-lactate dehydrogenase, D-(-)-lactate dehydrogenase (NAD), D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein, D-lactate dehydrogenase, D-lactic acid dehydrogenase, D-lactic dehydrogenase, D-LDH, D-LDH-like enzyme, D-LDH1, D-LDH2, D-LDH3, D-nLDH, D-specific lactic dehydrogenase, dehydrogenase, D-lactate, DLDH, DLDH744, ECBD_2243, ECLDH, Fermentative lactate dehydrogenase, FN0511, FNLDH, lactic acid dehydrogenase, LDH, ldhd, LdhTi, Ljd-LDH, NAD-dependent D-lactate dehydrogenase, PA0927, PALDH, Respiratory D-lactate dehydrogenase, WP_013906894

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.28 D-lactate dehydrogenase

Purification

Purification on EC 1.1.1.28 - D-lactate dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
copurification of recombinant His6-tagged D-lactate dehydrogenase and His6-tagged Ogataea parapolymorpha FDH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
partial
Allomyces sp.
-
recombinant D-LDH from Escherichia coli strain Rosetta (DE3) by ultracentrifugation, anion exchange and hydroxyapatite chromatography, followed by gel filtration
recombinant enzyme from Escherichia coli strain Rosetta-gami 2 (DE3) by nickel affnity chromatography and gel filtration
recombinant His-tagged enzyme 1.82fold from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by two different steps of affinity chromatography, and ultrafiltration
recombinant His-tagged selenomethionine-labeled enzyme from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatgraphy
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-