1.1.1.28: D-lactate dehydrogenase

This is an abbreviated version, for detailed information about D-lactate dehydrogenase, go to the full flat file.

Reaction

(R)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

D-(-)-lactate dehydrogenase, D-(-)-lactate dehydrogenase (NAD), D-lactate dehydrogenase, D-lactic acid dehydrogenase, D-lactic dehydrogenase, D-LDH, D-LDH1, D-LDH2, D-LDH3, D-specific lactic dehydrogenase, dehydrogenase, D-lactate, DLDH, Fermentative lactate dehydrogenase, lactic acid dehydrogenase, LDH, Respiratory D-lactate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.28 D-lactate dehydrogenase

Purification

Purification on EC 1.1.1.28 - D-lactate dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Allomyces sp.
-
recombinant D-LDH from Escherichia coli strain Rosetta (DE3) by ultracentrifugation, anion exchange and hydroxyapatite chromatography, followed by gel filtration
recombinant enzyme from Escherichia coli strain Rosetta-gami 2 (DE3) by nickel affnity chromatography and gel filtration
recombinant His-tagged enzyme 1.82fold from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-