Cloned (Comment) | Organism |
---|---|
gene lhdD or LEUM 1756, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Star | Leuconostoc mesenteroides subsp. mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not inhibited by Ca2+, Co2+, Cu2+, Mg2+, Mn2+, Na+, or urea | Leuconostoc mesenteroides subsp. mesenteroides | |
SDS | over 95% inhibition at 1 mM | Leuconostoc mesenteroides subsp. mesenteroides | |
Zn2+ | 70% inhibition at 1 mM | Leuconostoc mesenteroides subsp. mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Leuconostoc mesenteroides subsp. mesenteroides | |
0.58 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Leuconostoc mesenteroides subsp. mesenteroides | |
1.96 | - |
NAD+ | pH 11.0, 20°C, recombinant enzyme | Leuconostoc mesenteroides subsp. mesenteroides | |
260 | - |
(R)-lactate | pH 11.0, 20°C, recombinant enzyme | Leuconostoc mesenteroides subsp. mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides | - |
pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | - |
pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides subsp. mesenteroides | - |
gene ldhD or LEUM 1756 | - |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | - |
gene ldhD or LEUM 1756 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 1.82fold from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography | Leuconostoc mesenteroides subsp. mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.78 | - |
purified recombinant enzyme, D-lactate oxidation, pH 11.0, 20°C | Leuconostoc mesenteroides subsp. mesenteroides |
4450 | - |
purified recombinant enzyme, NADH oxidation, pH 8.0, 30°C | Leuconostoc mesenteroides subsp. mesenteroides |
4990 | - |
purified recombinant enzyme, pyruvate reduction, pH 8.0, 30°C | Leuconostoc mesenteroides subsp. mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | pyruvate + NADH + H+ | - |
r | |
(R)-lactate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | pyruvate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Leuconostoc mesenteroides subsp. mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
D-(-)-lactate dehydrogenase | - |
Leuconostoc mesenteroides subsp. mesenteroides |
LDH | - |
Leuconostoc mesenteroides subsp. mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
lactate oxidation reaction | Leuconostoc mesenteroides subsp. mesenteroides |
30 | - |
pyruvate reduction reaction | Leuconostoc mesenteroides subsp. mesenteroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | activity range, profile overview | Leuconostoc mesenteroides subsp. mesenteroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2280 | - |
(R)-lactate | pH 11.0, 20°C, recombinant enzyme | Leuconostoc mesenteroides subsp. mesenteroides | |
29000 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Leuconostoc mesenteroides subsp. mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
pyruvate reduction reaction | Leuconostoc mesenteroides subsp. mesenteroides |
11 | - |
lactate oxidation reaction | Leuconostoc mesenteroides subsp. mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | pyruvate reduction reaction, activity range, profile overview | Leuconostoc mesenteroides subsp. mesenteroides |
7 | 13 | lactate oxidation reaction, activity range, profile overview | Leuconostoc mesenteroides subsp. mesenteroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | |
NADH | - |
Leuconostoc mesenteroides subsp. mesenteroides |