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Literature summary for 1.1.1.28 extracted from

  • Wittenberger, C.L.
    D(-)-Lactate dehydrogenase from butyribacterium rettgeri (1975), Methods Enzymol., 41B, 299-303.
    View publication on PubMed
Search for more literature for 1.1.1.28

General Stability

General Stability Organism
repeated freezing and thawing, low ionic strength or pH above 6.5 cause loss of activity Eubacterium limosum

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Eubacterium limosum
Hg2+
-
 Eubacterium limosum
oxamate
-
 Eubacterium limosum
p-chloromercuriphenyl sulfonic acid
-
 Eubacterium limosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
 gel filtration Eubacterium limosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + NAD+ Eubacterium limosum
-
 pyruvate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Eubacterium limosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Eubacterium limosum

Storage Stability

Storage Stability Organism
0°C or -20°C, 0.5 M potassium phosphate buffer pH 6.2, two weeks Eubacterium limosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
 Eubacterium limosum pyruvate + NADH
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6.5
-
 Eubacterium limosum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Eubacterium limosum
NADH
-
 Eubacterium limosum