Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: LiCl, MnCl2, NiCl2, CoCl2, MgCl2, CuSO4, AlCl3, hydroxylamaine, hydrazine | Staphylococcus sp. | |
phenylhydrazine | up to 45% inhibition | Staphylococcus sp. | |
ZnSO4 | 1 mM, 80% inhibition, EDTA restores | Staphylococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
pyruvate | pH 8.0, 25°C | Staphylococcus sp. | |
2.3 | - |
2-oxobutyrate | pH 8.0, 25°C | Staphylococcus sp. | |
8.5 | - |
(R)-lactate | pH 8.0, 25°C | Staphylococcus sp. | |
19 | - |
DL-2-hydroxybutyrate | pH 8.0, 25°C | Staphylococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Staphylococcus sp. |
64000 | - |
gel filtration | Staphylococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus sp. | - |
isolated as high producer of enzyme, growth under anaerobic conditions | - |
Staphylococcus sp. LDH-1 | - |
isolated as high producer of enzyme, growth under anaerobic conditions | - |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1710 | - |
pH 8.0, 25°C | Staphylococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | - |
Staphylococcus sp. | pyruvate + NADH | - |
r | |
(R)-lactate + NAD+ | - |
Staphylococcus sp. LDH-1 | pyruvate + NADH | - |
r | |
2-oxobutyrate + NADH | 11% of activity with pyruvate | Staphylococcus sp. | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH | 11% of activity with pyruvate | Staphylococcus sp. LDH-1 | 2-hydroxybutyrate + NAD+ | - |
r | |
DL-2-hydroxybutyrate + NAD+ | 3% of activity with (R)-lactate | Staphylococcus sp. | 2-oxobutyrate + NADH | - |
r | |
DL-2-hydroxybutyrate + NAD+ | 3% of activity with (R)-lactate | Staphylococcus sp. LDH-1 | 2-oxobutyrate + NADH | - |
r | |
methylpyruvate + NADH | 84% of activity with pyruvate | Staphylococcus sp. | ? + NAD+ | - |
r | |
methylpyruvate + NADH | 84% of activity with pyruvate | Staphylococcus sp. LDH-1 | ? + NAD+ | - |
r | |
additional information | specific for NAD+/NADH | Staphylococcus sp. | ? | - |
? | |
additional information | specific for NAD+/NADH | Staphylococcus sp. LDH-1 | ? | - |
? | |
pyruvate + NADH + H+ | - |
Staphylococcus sp. | (R)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Staphylococcus sp. |
More | N-terminal amino acid sequence | Staphylococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Staphylococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
33% of activity at 40°C | Staphylococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
substrate pyruvate | Staphylococcus sp. |
8.2 | - |
substrate D-lactate | Staphylococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Staphylococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Staphylococcus sp. | |
NADH | - |
Staphylococcus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Staphylococcus sp. | isoelectric focusing | - |
4.2 |