1.1.1.28: D-lactate dehydrogenase

This is an abbreviated version, for detailed information about D-lactate dehydrogenase, go to the full flat file.

Reaction

(R)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

D-(-)-lactate dehydrogenase, D-(-)-lactate dehydrogenase (NAD), D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein, D-lactate dehydrogenase, D-lactic acid dehydrogenase, D-lactic dehydrogenase, D-LDH, D-LDH-like enzyme, D-LDH1, D-LDH2, D-LDH3, D-nLDH, D-specific lactic dehydrogenase, dehydrogenase, D-lactate, DLDH, DLDH744, ECBD_2243, ECLDH, Fermentative lactate dehydrogenase, FN0511, FNLDH, lactic acid dehydrogenase, LDH, ldhd, LdhTi, Ljd-LDH, NAD-dependent D-lactate dehydrogenase, PA0927, PALDH, Respiratory D-lactate dehydrogenase, WP_013906894

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.28 D-lactate dehydrogenase

Cloned

Cloned on EC 1.1.1.28 - D-lactate dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
co-overexpression of Pediococcus pentosaceus D-lactate dehydrogenase and Ogataea parapolymorpha FDH as His6-tagged enzymes in Escherichia coli strain BL21(DE3)
construction of a metabolically engineered Saccharomyces cerevisiae that produces D-lactic acid efficiently. Two copies of the D-lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. mesenteroides strain NBRC3426 are introduced into the genome
D-LDH isozymes D-LDH1, D-LDH2, and D-LDH3, sequence comparisons and phylogenetic analysis
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli as thioredoxin fusion protein
-
expression of D-LDH in Escherichia coli strain Rosetta (DE3)
expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene APE_0487, expression in Escherichia coli strain Rosetta-gami 2 (DE3)
gene ecd_01352, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3)
gene fn0511, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3)
gene ldhA, expression in an enzyme-deficient ldhA-null Corynebacterium glutamicum strain resulting in increased production of D-lactate
gene ldhD, phylogenetic analysis, recombinant expression of wwild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene ldhD, quantitative enzyme realtime PCR expression analysis during ferentation, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene ldhD, quantitative enzyme realtime PCR expression analysis during fermentation, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ldhD, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene ldhD, subcloning in Escherichia coli straiin DH5alpha, functional overexpression of phenyllactic acid-overproducing enzyme mutant in Escherichia coli strain BL21 (DE3), optimal induction at 25C
-
gene lhdD or LEUM 1756, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Star
-
gene pa0927, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3)
gene WP_013906894 , recombinant expression of the enzyme in Bacillus licheniformis strain ATCC 14580 MW3 derivative BL2 with a deleted native gene ldh, encoding L-LDH. Gene WP_013906894 is introduced to redirect the carbon flux, the genes for 2,3-butanediol production, alsS and alsD, are replaced with WP_013906894, giving strains BN11, BN22, BN44, and BN66
recombinant expression of His-tagged selenomethionine-labeled enzyme in Escherichia coli strain B834
-
Use of the addressing sequence of yeast D-lactate dehydrogenase for insertion of CYP11A1p into the inner membrane of yeast mitochondria
-