1.1.1.28: D-lactate dehydrogenase

This is an abbreviated version, for detailed information about D-lactate dehydrogenase, go to the full flat file.

Reaction

(R)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

D-(-)-lactate dehydrogenase, D-(-)-lactate dehydrogenase (NAD), D-lactate dehydrogenase, D-lactic acid dehydrogenase, D-lactic dehydrogenase, D-LDH, D-LDH1, D-LDH2, D-LDH3, D-specific lactic dehydrogenase, dehydrogenase, D-lactate, DLDH, Fermentative lactate dehydrogenase, lactic acid dehydrogenase, LDH, Respiratory D-lactate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.28 D-lactate dehydrogenase

Application

Application on EC 1.1.1.28 - D-lactate dehydrogenase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
construction of a metabolically engineered Saccharomyces cerevisiae that produces D-lactic acid efficiently. Two copies of the D-lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. mesenteroides strain NBRC3426 are introduced into the genome. The D-lactate production reaches 61.5 g/l, the amount of glucose being transformed into D-lactic acid is 53.0% under non-neutralizing conditions. The D-lactic acid is of extremely high optical purity of 99.9% or higher
diagnostics
-
the enzyme is useful for selective determination of D,L-lactic acid in wines using peroxidase-based biosensors, method optimization, overview
molecular biology
synthesis