Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lactobacillus delbrueckii subsp. bulgaricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | 1.6 | pyruvate | recombinant and wild-type enzyme | Lactobacillus delbrueckii subsp. bulgaricus | |
7.5 | 9 | oxaloacetate | recombinant and wild-type enzyme | Lactobacillus delbrueckii subsp. bulgaricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Lactobacillus delbrueckii subsp. bulgaricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | Lactobacillus delbrueckii subsp. bulgaricus | - |
pyruvate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus delbrueckii subsp. bulgaricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1950 | 2100 | - |
Lactobacillus delbrueckii subsp. bulgaricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + NAD+ | - |
Lactobacillus delbrueckii subsp. bulgaricus | pyruvate + NADH | - |
? | |
oxaloacetate + NADH | - |
Lactobacillus delbrueckii subsp. bulgaricus | malate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Lactobacillus delbrueckii subsp. bulgaricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Lactobacillus delbrueckii subsp. bulgaricus |