1.1.1.184: carbonyl reductase (NADPH)

This is an abbreviated version, for detailed information about carbonyl reductase (NADPH), go to the full flat file.

Reaction

R-CHOH-R'
+
NADP+
=
R-CO-R'
+
NADPH
+
H+

Synonyms

15-hydroxyprostaglandin dehydrogenase [NADP+], 2,5-diketo-D-gluconic acid reductase, Adipocyte P27 protein, aldehyde reductase 1, aldehyde reductase I, aldo-keto reductase, ALR3, AP27, carbonyl reductase, carbonyl reductase (NADPH), carbonyl reductase 1, carbonyl reductase 3, carbonyl reductase S1, CBR, CBR 1, CBR 3, CBR1, CBR3, CHCR, CHCR1, CHCR2, CHCR3, CR, CSCR1, Gox0644, Gox1615, ketoreductase, KLCR1, KR, LCR, microsomal carbonyl reductase, More, NADP+-dependent ADH, NADPH-carbonyl reductase, NADPH-dependent carbonyl reductase, NADPH-dependent carbonyl reductase S1, NCCR, nonspecific NADPH-dependent carbonyl reductase, peroxisomal-type carbonyl reductase, PHCR, prostaglandin 9-ketoreductase, Prostaglandin-E2 9-reductase, PTCR, R-specific carbonyl reductase, reductase S1, reductase, carbonyl, RLCR, SCR, SDR21C1, secondary-alcohol: NADP+-oxidoreductase, short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase, short-chain carbonyl reductase, sniffer, SSCR, Tm1743, Tm_1743, xenobiotic carbonyl reductase, xenobiotic ketone reductase, YtbE

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.184 carbonyl reductase (NADPH)

Activating Compound

Activating Compound on EC 1.1.1.184 - carbonyl reductase (NADPH)

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-propanol
-
increases conversion, giving the conversion of 90% compared to 9% in control at a substrate concentration of 70 mM in 1 h. Operational stability increases at higher temperatures with the addition of 2-propanol in the reaction mixture with good conversion (90%) and enantiomeric excess (more than 99%) at 45°C and 50°C. It may be used as a co-substrate for the bioreduction reaction, which may help in widening its applicability in terms of substrate tolerance, reduced time of conversion, increased conversion rate and thermostability
Anionic detergents
-
e.g. SDS and sarkosyl stimulate
-
EDTA
activtion to 112% activity; up to 112% of initial activity
fatty acids
-
with carbon chain length greater than nine at pH 7.0 activate, e.g. arachidonic acid
hexane
-
activity retention is highest in the presence of hexane, reaction shows maximum conversion and enantiomeric excess of 96 and more than 99%, respectively in 15%, v/v hexane with 2-propanol as the cosubstrate
additional information
-