1.1.1.184: carbonyl reductase (NADPH)

This is an abbreviated version, for detailed information about carbonyl reductase (NADPH), go to the full flat file.

Reaction

R-CHOH-R'
+
NADP+
=
R-CO-R'
+
NADPH
+
H+

Synonyms

15-hydroxyprostaglandin dehydrogenase [NADP+], 2,5-diketo-D-gluconic acid reductase, Adipocyte P27 protein, aldehyde reductase 1, aldehyde reductase I, aldo-keto reductase, ALR3, AP27, carbonyl reductase, carbonyl reductase (NADPH), carbonyl reductase 1, carbonyl reductase 3, carbonyl reductase S1, CBR, CBR 1, CBR 3, CBR1, CBR3, CHCR, CHCR1, CHCR2, CHCR3, CR, CSCR1, Gox0644, Gox1615, ketoreductase, KLCR1, KR, LCR, microsomal carbonyl reductase, More, NADP+-dependent ADH, NADPH-carbonyl reductase, NADPH-dependent carbonyl reductase, NADPH-dependent carbonyl reductase S1, NCCR, nonspecific NADPH-dependent carbonyl reductase, peroxisomal-type carbonyl reductase, PHCR, prostaglandin 9-ketoreductase, Prostaglandin-E2 9-reductase, PTCR, R-specific carbonyl reductase, reductase S1, reductase, carbonyl, RLCR, SCR, SDR21C1, secondary-alcohol: NADP+-oxidoreductase, short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase, short-chain carbonyl reductase, sniffer, SSCR, Tm1743, Tm_1743, xenobiotic carbonyl reductase, xenobiotic ketone reductase, YtbE

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.184 carbonyl reductase (NADPH)

Temperature Stability

Temperature Stability on EC 1.1.1.184 - carbonyl reductase (NADPH)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 60
purified recombinant enzyme, stable for 15 h
35
-
stable for 60 min
45
melting temperature, mutant Y187A
46
melting temperature, mutant S172A; melting temperature, mutant Y187F
48
melting temperature, mutant S172T and N-terminal deletion mutant lacking 31 amino acids
50
-
pH 6-11, 15 min, about 20% loss of activity
52
melting temperature, wild-type
60
-
rapid inactivation above
65
Candida macedoniensis
-
pH 7.0, 0.1 mM DTT, complete loss of activity after 10 min
85
15 h, 63% residual activity; the purified recombinant enzyme retains 63% of its maximal activity after 15 h at 85°C
additional information
-
activation by arachidonic acid leads to a decrease in thermal stability