1.1.1.184: carbonyl reductase (NADPH)

This is an abbreviated version, for detailed information about carbonyl reductase (NADPH), go to the full flat file.

Reaction

R-CHOH-R'
+
NADP+
=
R-CO-R'
+
NADPH
+
H+

Synonyms

15-hydroxyprostaglandin dehydrogenase [NADP+], 2,5-diketo-D-gluconic acid reductase, Adipocyte P27 protein, aldehyde reductase 1, aldehyde reductase I, aldo-keto reductase, ALR3, AP27, carbonyl reductase, carbonyl reductase (NADPH), carbonyl reductase 1, carbonyl reductase 3, carbonyl reductase S1, CBR, CBR 1, CBR 3, CBR1, CBR3, CHCR, CHCR1, CHCR2, CHCR3, CR, CSCR1, Gox0644, Gox1615, ketoreductase, KLCR1, KR, LCR, microsomal carbonyl reductase, More, NADP+-dependent ADH, NADPH-carbonyl reductase, NADPH-dependent carbonyl reductase, NADPH-dependent carbonyl reductase S1, NCCR, nonspecific NADPH-dependent carbonyl reductase, peroxisomal-type carbonyl reductase, PHCR, prostaglandin 9-ketoreductase, Prostaglandin-E2 9-reductase, PTCR, R-specific carbonyl reductase, reductase S1, reductase, carbonyl, RLCR, SCR, SDR21C1, secondary-alcohol: NADP+-oxidoreductase, short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase, short-chain carbonyl reductase, sniffer, SSCR, Tm1743, Tm_1743, xenobiotic carbonyl reductase, xenobiotic ketone reductase, YtbE

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.184 carbonyl reductase (NADPH)

Purification

Purification on EC 1.1.1.184 - carbonyl reductase (NADPH)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms: major form CR2, minor form CR1
-
2 enzyme species
-
2 forms from vas deferens: V1, V2; 3 forms from testis: T1, T2, T3
-
3 forms from liver: CR1, CR2, CR3
-
3 forms from testis: CR1, CR2, CR3
-
3 forms from testis: T1, T2, T3
-
3 molecular forms: CR7, CR8, CR8.5
-
380fold to homogeneity by protamine precipitation, solubilization, ammonium sulfate fractionation, solubilization with 0.5 M NaCl, ultrafiltration, butyl-resin chromatography, and gel filtration
-
487fold to homogeneity in a 4-step procedure
-
by affinity chromatography on a Ni2+ column and by gel filtration
by metal-chelating affinity column chromatography and gel filtration; by metal-chelating affinity column chromatography and gel filtration; by metal-chelating affinity column chromatography and gel filtration
by Ni-NTA affinity chromatography
-
by Ni2+ chelating affinity column chromatography
by sequential column chromatographies involving DEAE ion exchange, phenyl resin, and hydroxylapatite chromatography
Cylindrocarpon sclerotigenum
-
by several chromatographic steps, i.e. Q-Sepharose, FPLC, Phenyl Sepharose, HiTrap Blue, Red Sepharose, and gel filtration, and native PAGE
-
mutants purfied by Ni-affinity chromatography
native enzyme from liver microsomes to homogeneity
-
recombinant enzyme
-
recombinant enzyme 3.1fold from Escherichia coli strain BL21(DE3)
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, and affinity and hydroxylapatite chromatography, followed by ultrafiltration, the protein easily precipitates at concentraions of above 3.3 mg/ml
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography
-
recombinant enzyme from Escherichia coli strain BL21(DE3) partially by ammonium sulfate fractionation
-
recombinant His-tagged active enzyme from Escherichia coli
-
recombinant His-tagged enzyme 17.8fold from Escherichia coli strain BL21(DE3) by affinity chromatography
recombinant His-tagged enzyme by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged isozymes CHCR1-3 from Escherichia coli strain BL21(DE3)
-
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity and anion exchange chromatography, cleavage of the tag by thrombin protease, and gel filtration
recombinant protein
recombinant protein purified from Escherichia coli
-
recombinant SSCR from Escherichia coli strain BL21(DE3)
-
recombinant wild-type and mutant enzymes from Escherichia coli strain HB101 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, to homogeneity
-
recombinant wild-type enzyme from Escherichia coli strain JM109 and recombinant selenomethionine-CR from Escherichia coli strain B834, by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration
-
wild-type and mutant enzymes purified to homogeneity by Ni-affinity chromatography