1.1.1.184: carbonyl reductase (NADPH)

This is an abbreviated version, for detailed information about carbonyl reductase (NADPH), go to the full flat file.

Reaction

R-CHOH-R'
+
NADP+
=
R-CO-R'
+
NADPH
+
H+

Synonyms

15-hydroxyprostaglandin dehydrogenase [NADP+], 2,5-diketo-D-gluconic acid reductase, Adipocyte P27 protein, aldehyde reductase 1, aldehyde reductase I, aldo-keto reductase, ALR3, AP27, carbonyl reductase, carbonyl reductase (NADPH), carbonyl reductase 1, carbonyl reductase 3, carbonyl reductase S1, CBR, CBR 1, CBR 3, CBR1, CBR3, CHCR, CHCR1, CHCR2, CHCR3, CR, CSCR1, Gox0644, Gox1615, ketoreductase, KLCR1, KR, LCR, microsomal carbonyl reductase, More, NADP+-dependent ADH, NADPH-carbonyl reductase, NADPH-dependent carbonyl reductase, NADPH-dependent carbonyl reductase S1, NCCR, nonspecific NADPH-dependent carbonyl reductase, peroxisomal-type carbonyl reductase, PHCR, prostaglandin 9-ketoreductase, Prostaglandin-E2 9-reductase, PTCR, R-specific carbonyl reductase, reductase S1, reductase, carbonyl, RLCR, SCR, SDR21C1, secondary-alcohol: NADP+-oxidoreductase, short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase, short-chain carbonyl reductase, sniffer, SSCR, Tm1743, Tm_1743, xenobiotic carbonyl reductase, xenobiotic ketone reductase, YtbE

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.184 carbonyl reductase (NADPH)

General Stability

General Stability on EC 1.1.1.184 - carbonyl reductase (NADPH)

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activation by arachidonic acid leads to a decrease in thermal stability
-
an ammonium sulfate precipitate (3 M, pH 7) of the enzyme loses its activity completely within a few days
-
compared to activity in acetate and Tris-HCl, carbonyl reductase shows higher activity using potassium phosphate as buffer at pH 5.5 or pH 7
-
glycerol, 20% v/v, stabilizes
-
minor loss of activity in 50 mM phosphate buffer is observed during purification and storage at 4C without additional precautions
-
no conditions to stabilize the enzyme by freezing at -20C are found. Addition of 1-10% bovine serum albumin, 20-50% glycerol, 20% 1,2-ethanediol or 0.1% Triton X-100 to the enzyme solution in 40 mM phosphate or 40 mM Tris-HC1 pH 7 before freezing for 1 day or longer has no stabilizing effect
-