Information on EC 4.4.1.13 - cysteine-S-conjugate beta-lyase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.4.1.13
-
RECOMMENDED NAME
GeneOntology No.
cysteine-S-conjugate beta-lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-aminoprop-2-enoate = 2-iminopropanoate
show the reaction diagram
spontaneous
-
-
-
2-iminopropanoate + H2O = pyruvate + NH3
show the reaction diagram
spontaneous
-
-
-
an L-cysteine-S-conjugate + H2O = a thiol + NH3 + pyruvate
show the reaction diagram
an L-cysteine-S-conjugate = a thiol + 2-aminoprop-2-enoate
show the reaction diagram
(1a)
-
-
-
L-cystathionine + H2O = L-homocysteine + pyruvate + NH3
show the reaction diagram
L-cystathionine = L-homocysteine + 2-aminoprop-2-enoate
show the reaction diagram
(1a)
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
of H2S or RSH, C-S bond cleavage; of RSH, C-S bond cleavage
-
transamination
additional information
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glucosinolate biosynthesis from dihomomethionine
-
-
glucosinolate biosynthesis from hexahomomethionine
-
-
glucosinolate biosynthesis from homomethionine
-
-
glucosinolate biosynthesis from pentahomomethionine
-
-
glucosinolate biosynthesis from phenylalanine
-
-
glucosinolate biosynthesis from tetrahomomethionine
-
-
glucosinolate biosynthesis from trihomomethionine
-
-
glucosinolate biosynthesis from tryptophan
-
-
glucosinolate biosynthesis from tyrosine
-
-
glutathione-mediated detoxification II
-
-
homocysteine and cysteine interconversion
-
-
seleno-amino acid biosynthesis (plants)
-
-
methionine metabolism
-
-
Selenocompound metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
L-cysteine-S-conjugate thiol-lyase (deaminating; pyruvate-forming)
A pyridoxal-phosphate protein. The enzyme can act on a broad range of L-cysteine-S-conjugates, including aromatic conjugates such as 4-bromobenzene and 2,4-dinitrobenzene. The enzyme cleaves a carbon-sulfur bond, releasing a thiol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase.
CAS REGISTRY NUMBER
COMMENTARY hide
68652-57-3
-
9055-05-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
41A
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
subsp. bulgaricus
-
-
Manually annotated by BRENDA team
Monieza expansa
tapeworm
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain DSM 20701
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
S-methyl-L-cysteine-adapted
-
-
Manually annotated by BRENDA team
FM518
-
-
Manually annotated by BRENDA team
FM518
-
-
Manually annotated by BRENDA team
L. cv. Desiree
Uniprot
Manually annotated by BRENDA team
strains AX3 and JCSC1435, gene metC or SH2636
-
-
Manually annotated by BRENDA team
-
Q9EYM7
SwissProt
Manually annotated by BRENDA team
-
Q9EYM7
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
bifunctional cystine C-S lyase and alanine aminotransferase, EC 2.6.1.2
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(H2N)2Pt(Cl)SCH2CH(NH3+)CO2- + H2O
CH3C(O)CO2- + NH4+ + (NH2)2Pt(Cl)SH
show the reaction diagram
(S)-1,1,2,2-tetrafluoroethyl-L-cysteine + H2O
? + NH3 + pyruvate
show the reaction diagram
-
-
-
-
?
(S)-2,4-dinitrophenyl-L-cysteine
2,4-dinitrobenzenethiol + pyruvate + NH3
show the reaction diagram
(S)-2,4-dinitrophenyl-L-homocysteine
?
show the reaction diagram
(S)-2-benzothiazolyl-L-cysteine
pyruvate + NH3 + 2-mercaptobenzothiazole
show the reaction diagram
(S)-2-benzothiazolyl-L-cysteine + H2O
pyruvate + NH3 + 2-mercaptobenzothiazole
show the reaction diagram
-
-
-
-
?
(S)-4-(4-methylpentan-2-one)-L-cysteine + H2O
4-mercapto-4-methylpentan-2-one + NH3 + pyruvate
show the reaction diagram
-
Str3p is able to release 0.0123 mM 4-mercapto-4-methylpentan-2-one from 2 mM concentration of its precursor S-(2-methyl-4-oxopentan-2-yl)-L-cysteine
-
?
(S)-4-bromophenyl-L-cysteine
p-bromobenzenethiol + NH3 + pyruvate
show the reaction diagram
(S)-4-nitrobenzyl-L-cysteine
?
show the reaction diagram
-
-
-
-
?
(S)-methylcysteine + H2O
methanethiol + pyruvate + NH3
show the reaction diagram
2-(2-(S)-L-cysteinylpentyl)-1,3-thiazolidine-4-carboxylic acid
3-mercaptohexanal + ?
show the reaction diagram
-
-
-
?
3-cysteinylhexanol
3-mercaptohexanal + ?
show the reaction diagram
3-hydroxykynurenine
?
show the reaction diagram
3-S-(N-acetyl-L-cysteinyl)-hexanal
3-mercaptohexanal + ?
show the reaction diagram
3-S-L-cysteinylhexanal
3-mercaptohexanal + ?
show the reaction diagram
4,5-epoxy-4,5-dihydro-1-nitropyrene
?
show the reaction diagram
-
-
-
-
?
5'-S-cysteinyldopamine
?
show the reaction diagram
-
-
-
-
?
5-S-L-cysteinyldopamine
pyruvate + ?
show the reaction diagram
-
slow beta-elimination reaction
-
-
?
8-S-cysteinyl-p-menthan-3-one
8-mercapto-p-menthan-3-one
show the reaction diagram
-
-
-
?
9,10-epoxy-9,10-dihydro-1-nitropyrene
?
show the reaction diagram
-
-
-
-
?
alanine derivatives
?
show the reaction diagram
-
-
-
-
?
alpha-keto-gamma-methiobutyric acid + L-aspartate
?
show the reaction diagram
Monieza expansa
-
transamination reaction
-
?
benzothiazolyl-L-cysteine
?
show the reaction diagram
benzyl-L-cysteine
?
show the reaction diagram
-
-
-
?
beta-chloro-DL-alanine
?
show the reaction diagram
-
-
-
-
?
beta-chloro-L-alanine
?
show the reaction diagram
-
-
-
?
beta-chloroalanine
?
show the reaction diagram
-
-
-
-
?
cystathionine + H2O
?
show the reaction diagram
cystathionine + H2O
L-homocysteine + NH3 + pyruvate
show the reaction diagram
cysteine thioethers
?
show the reaction diagram
-
-
-
-
?
cystine + H2O
pyruvate + NH3 + thiocysteine
show the reaction diagram
-
-
-
-
?
D-Cys + H2O
?
show the reaction diagram
-
-
-
-
?
D-cystine + H2O
?
show the reaction diagram
-
2% of the activity with L-cystine
-
?
DL-homocysteine + H2O
hydrogen sulfide + 2-oxobutyrate + ?
show the reaction diagram
homoserine + H2O
?
show the reaction diagram
-
96% of the activity with L-cystathionine
-
-
?
kynurenine
?
show the reaction diagram
L-alanine
?
show the reaction diagram
L-aspartate
?
show the reaction diagram
L-cystathionine
L-homocysteine + pyruvate + NH4+
show the reaction diagram
L-cystathionine + H2O
cysteine + ?
show the reaction diagram
-
alpha,gamma-elimination
-
?
L-cystathionine + H2O
L-homocysteine + NH3 + pyruvate
show the reaction diagram
L-cystathionine + H2O
L-homocysteine + pyruvate + NH3
show the reaction diagram
L-cysteine + H2O
?
show the reaction diagram
L-cysteine + H2O
hydrogen sulfide + pyruvate + ?
show the reaction diagram
L-cysteine + H2O
sulfide + NH3 + pyruvate
show the reaction diagram
L-cysteine conjugates
?
show the reaction diagram
L-cysteine S-sulfate + H2O
?
show the reaction diagram
-
11% of the activity with L-cystine
-
?
L-cysteine-S-sulphate
?
show the reaction diagram
-
-
-
?
L-cystine
?
show the reaction diagram
-
-
-
?
L-cystine + H2O
?
show the reaction diagram
22% activity compared to L-cystathionine
-
-
?
L-cystine + H2O
L-cysteine persulfide + pyruvate + NH4+
show the reaction diagram
L-cystine + H2O
thiocysteine + pyruvate + NH3
show the reaction diagram
L-djenkolate
?
show the reaction diagram
-
-
-
-
-
L-djenkolate
pyruvate + NH3 + S-(mercaptomethyl)cysteine
show the reaction diagram
L-djenkolate + H2O
pyruvate
show the reaction diagram
L-djenkolate + H2O
pyruvate + NH3 + ?
show the reaction diagram
most effective substrate, 154% activity compared to L-cystathionine
-
-
?
L-djenkolate sulphoxide
?
show the reaction diagram
-
-
-
?
L-djenkolic acid + H2O
?
show the reaction diagram
-
18-24% of the activity with L-cystine, depending on assay method
-
?
L-homocysteine + H2O
?
show the reaction diagram
L-homolanthionine + H2O
?
show the reaction diagram
-
-
-
-
?
L-leucine + alpha-ketoglutarate
?
show the reaction diagram
-
-
-
?
L-meso-lanthionine + H2O
?
show the reaction diagram
-
-
-
-
?
L-methionine
methanethiol + (2S)-2-aminobutanoic acid
show the reaction diagram
-
-
-
-
?
L-methionine + H2O
methanethiol + dimethyl disulfide + dimethyl trisulfide + 2-oxobutyrate
show the reaction diagram
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
show the reaction diagram
L-selenocystathionine + H2O
?
show the reaction diagram
L-Selenodjenkolate
Se-(hydroselenomethyl)selenocysteine + pyruvate + NH3
show the reaction diagram
L-selenomethionine
HSe(CH3) + NH3 + 2-oxobutanoate
show the reaction diagram
-
-
-
?
L-serine + H2O
hydrogen sulfide + pyruvate + NH3
show the reaction diagram
MalY
-
-
?
lanthionine + H2O
L-cysteine + NH3 + pyruvate
show the reaction diagram
leukotriene E4
?
show the reaction diagram
-
-
-
-
?
mixed disulfide of L-cysteine and L-homocysteine + H2O
?
show the reaction diagram
-
-
-
-
?
O-acetyl-L-serine + H2O
acetate + pyruvate + NH3
show the reaction diagram
-
-
-
-
?
O-methyl-DL-serine
?
show the reaction diagram
-
-
-
?
O-succinyl-L-homoserine + H2O
2-oxobutyrate + succinate + NH3
show the reaction diagram
MetC
-
-
?
p-nitrobenzyl-L-cysteine
?
show the reaction diagram
RS-CH2-CH(NH3+)COO- + H2O
RSH + NH3 + pyruvate
show the reaction diagram
RSH-CH2-CH(NH3+)COO-
RSH + NH3 + pyruvate
show the reaction diagram
RSH-CH2-CH(NH3+)COO- + H2O
RSH + NH3 + pyruvate
show the reaction diagram
-
-
-
-
?
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
?
show the reaction diagram
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
NH3 + pyruvate + 1,1,2,2-tetrafluoroethanethiol
show the reaction diagram
-
-
-
-
?
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine + H2O
NH3 + pyruvate + 1,1,2,2-tetrafluoroethanethiol
show the reaction diagram
-
-
-
-
ir
S-(1,1,2-trichlorovinyl)-L-cysteine
?
show the reaction diagram
Monieza expansa
-
-
-
?
S-(1,2-dichloroethenyl)-L-cysteine
pyruvate + NH3 + dichlorovinyl mercaptane
show the reaction diagram
-
-
-
-
?
S-(1,2-dichlorovinyl)-L-cysteine
?
show the reaction diagram
S-(1,2-dichlorovinyl)-L-cysteine + H2O
NH3 + pyruvate + 1,2-dichloroethylenethiol
show the reaction diagram
S-(1-hydroxyhexan-3-yl)-L-cysteine + H2O
3-mercaptohexan-1-ol + NH3 + pyruvate
show the reaction diagram
-
Str3p is able to release 0.0021 mM 3-mercaptohexan-1-ol from 2 mM concentrations of its precursor (S)-3-(hexan-1-ol)-L-cysteine
-
?
S-(2-aminoethyl)-L-Cys + H2O
?
show the reaction diagram
-
-
-
-
?
S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
?
show the reaction diagram
S-(E-1,2-Dichlorovinyl)-L-cysteine
?
show the reaction diagram
S-(p-bromophenyl)-L-cysteine
?
show the reaction diagram
-
-
-
?
S-(p-bromophenyl)-L-cysteine
p-bromobenzenethiol + NH3 + pyruvate
show the reaction diagram
-
-
-
?
S-1,2-dichlorovinyl-L-cysteine + H2O
dichlorovinyl mercaptane + NH3 + pyruvate
show the reaction diagram
-
-
-
-
?
S-1,2-dichlorovinyl-L-cysteine + H2O
pyruvate + NH3 + dichlorovinyl mercaptane
show the reaction diagram
-
-
-
-
?
S-4-(4-methylpentan-2-one)-L-cysteine + H2O
4-mercapto-4-methylpentan-2-one + NH3 + pyruvate
show the reaction diagram
-
-
-
-
?
S-alkyl cysteines
?
show the reaction diagram
-
-
-
-
?
S-allyl-L-cysteine + H2O
NH3 + pyruvate + prop-2-ene-1-thiol
show the reaction diagram
-
-
-
-
ir
S-allylmercapto-L-cysteine + H2O
NH3 + pyruvate + allyl hydrodisulfide
show the reaction diagram
-
-
-
-
ir
S-Aralkyl cysteines
?
show the reaction diagram
-
-
-
-
?
S-carbamoyl-L-cysteine
?
show the reaction diagram
-
-
-
?
S-ethyl-L-cysteine
?
show the reaction diagram
S-ethyl-L-cysteine + H2O
?
show the reaction diagram
-
18% of the activity with L-cystine
-
?
S-ethyl-L-cysteine sulfoxide + H2O
?
show the reaction diagram
-
71% of the activity with L-cystine
-
?
S-methyl-L-Cys + H2O
?
show the reaction diagram
-
-
-
-
?
S-methyl-L-cysteine
methyl mercaptane + pyruvate + NH3
show the reaction diagram
S-methyl-L-cysteine + H2O
?
show the reaction diagram
S-methyl-L-cysteine sulfoxide
methyl methanethiolsulfinate + pyruvate + NH3
show the reaction diagram
S-methylcysteine + H2O
?
show the reaction diagram
-
-
-
-
?
S-methylmercapto-L-cysteine + H2O
NH3 + pyruvate + methanethiol
show the reaction diagram
-
-
-
-
ir
S-penta-1,3-dienylmercapto-L-cysteine + H2O
NH3 + pyruvate + penta-1,3-dien-1-yl hydrodisulfide
show the reaction diagram
-
-
-
-
ir
S-propyl-L-cysteine
?
show the reaction diagram
-
-
-
?
S-propyl-L-cysteine + H2O
?
show the reaction diagram
-
29% of the activity with L-cystine
-
?
S-propylmercapto-L-cysteine + H2O
NH3 + pyruvate + propyl hydrodisulfide
show the reaction diagram
-
-
-
-
ir
S-[1-(2-hydroxymethyl)-1-methylbutyl]-L-cysteine + H2O
sulfanylhexanol + ?
show the reaction diagram
-
very low activity
-
-
?
S-[1-(2-hydroxymethyl)-1-methylbutyl]-L-cysteinylglycine + H2O
sulfanylhexanol + ?
show the reaction diagram
-
very low activity
-
-
?
S-[2-(fluoromethoxy)-1,1,3,3,3-pentafluoropropyl]-L-cysteine
pyruvate + ?
show the reaction diagram
-
-
-
?
S-[2-(fluoromethoxy)-1,3,3,3-tetrafluoropropyl]-L-cysteine
pyruvate + ?
show the reaction diagram
-
-
-
?
Se-(4-methylbenzyl)-L-selenocysteine
?
show the reaction diagram
-
-
-
?
Se-(allyl)-L-selenocysteine
?
show the reaction diagram
-
-
-
?
Se-(hydroselenomethyl)selenocysteine
bis(hydroseleno)methane + pyruvate + NH3
show the reaction diagram
-
-
-
ir
Se-(isopropyl)-L-selenocysteine
?
show the reaction diagram
-
-
-
?
Se-(phenyl)-L-selenocysteine
?
show the reaction diagram
-
-
-
?
Se-4-methoxybenzylselenocysteine + H2O
HSe(4-(CH3O)C6H4CH2) + pyruvate + NH3
show the reaction diagram
Se-methyl-L-selenocysteine
?
show the reaction diagram
-
-
-
-
?
Se-methyl-L-selenocysteine + H2O
HSe(CH3) + pyruvate + NH3
show the reaction diagram
-
-
-
?
Se-methylselenocysteine + H2O
HSe(CH3) + pyruvate + NH3
show the reaction diagram
Te-phenyl-L-tellurocysteine + H2O
NH3 + pyruvate + phenyltelluride
show the reaction diagram
-
-
-
-
ir
thioethers
?
show the reaction diagram
xenobiotics
thiol + pyruvate + NH3
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cystathionine + H2O
?
show the reaction diagram
L-cystathionine + H2O
L-homocysteine + NH3 + pyruvate
show the reaction diagram
L-cystathionine + H2O
L-homocysteine + pyruvate + NH3
show the reaction diagram
L-cysteine + H2O
sulfide + NH3 + pyruvate
show the reaction diagram
A6YH85
-
-
-
?
L-djenkolate
?
show the reaction diagram
-
-
-
-
-
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
show the reaction diagram
A6YH85
-
-
-
?
RS-CH2-CH(NH3+)COO- + H2O
RSH + NH3 + pyruvate
show the reaction diagram
-
-
-
-
ir
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
additional information
-
no cofactor requirement
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-(4-chlorophenyl)-3-[4-(6-methyl-1,3-benzothiazol-2-yl)phenyl]urea
-
reversible inhibition
1-[3-(3,4-dichlorophenyl)isoxazol-5-yl]-2-[(4-thiophen-2-ylpyrimidin-2-yl)sulfanyl]ethanone
-
reversible inhibition
2,3,5-trifluoro-N-(2-hydrazino-2-oxoethyl)-4-methoxybenzamide
-
only poor inhibitor
2-bromobenzyl (9Z)-9-(hydroxyimino)-2,7-dinitro-9H-fluorene-4-carboxylate
-
hydrolysis of the oxime to generate hydroxylamine in situ that interacted with the PLP cofactor
3,3,3,-trifluoroalanine
3,3,3-trifluoro-N-(2-methylphenyl)-2-(trifluoromethyl)propanamide
-
reversible inhibition
3,4-dichlorobenzenesulfonohydrazide
-
known to interact with the cofactor of PLP enzymes
3,5-diamino-N-(2-hydrazino-2-oxoethyl)benzamide
-
-
3-amino-N-(2-hydrazino-2-oxoethyl)naphthalene-2-carboxamide
-
only poor inhibitor
3-hydroxykynurenine
-
cysteine conjugate beta-lyase activity
3-methyl-2-benzothiazolinone hydrazoone
-
-
4-chloro-N-[4-(6-methyl-1,3-benzothiazol-2-yl)phenyl]benzamide
-
reversible inhibition
4-methylbenzenesulfonohydrazide
-
known to interact with the cofactor of PLP enzymes
5,5'-dithiobis(2-nitrobenzoic acid)
5-aminonaphthalene-1-sulfonohydrazide
-
known to interact with the cofactor of PLP enzymes
5-chlorothiophene-2-sulfonohydrazide
-
known to interact with the cofactor of PLP enzymes
5-pyridin-2-ylthiophene-2-sulfonohydrazide
-
known to interact with the cofactor of PLP enzymes
aminoethoxyvinylglycine
aminooxyacetic acid
aminooxyacetic acid hemihydrochloride
ammonium sulfate
-
66% inhibition at 4 mM
aromatic disulfide
-
-
-
Beta-cyanoalanine
carboxymethoxylamine
-
irreversible
diphenyl 9-(dicyanomethylidene)-4,5-dinitro-9H-fluorene-2,7-disulfonate
-
reversible inhibition
DL-cycloserine
-
irreversible
DL-homocysteine
-
12.5 mM, 70% inhibition, competitive inhibitor
DL-Penicillamine
-
-
DL-propargylglycine
DTT
-
10 mM, 19% inhibition
glutathione
-
strong
glycine
-
73% inhibition at 4 mM
HgCl2
-
57% inhibition at 1 mM
homocysteine
-
strong
hydoxylamine
-
complete inhibition at 1 mM
hydroxylamine
iodoacetamide
iodoacetate
kynurenine
-
cysteine conjugate beta-lyase activity
L-alpha-(2-aminoethoxyvinyl)glycine
L-aminoethoxyvinylglycine
-
time-dependent slow-binding, one-step mechanism
L-canaline
-
1 mM, 67% inhibition, 10 mM complete inhibition
L-cystathionine
-
substrate inhibition at concentrations above 6 mM
L-cysteine
-
12 mM, 65% inhibition after 5 min, 1 mM, 29% inhibition after 10 min, linear noncompetitive inhibitor
L-Met
-
competitive
L-methionine
-
40% inhibition at 6 mM
L-serine
-
86% inhibition at 4 mM
N-(2-hydrazino-2-oxoethyl)-2,6-dimethoxybenzamide
-
-
N-(2-hydrazino-2-oxoethyl)-2-(trifluoromethyl)benzamide
-
-
N-(2-hydrazino-2-oxoethyl)-2-naphthalen-1-ylacetamide
-
-
N-(2-hydrazino-2-oxoethyl)-2-nitrobenzamide
-
known to interact with the cofactor of PLP enzymes
N-(2-hydrazino-2-oxoethyl)-3,5-dinitrobenzamide
-
-
N-(2-hydrazino-2-oxoethyl)-3-(trifluoromethyl)benzamide
-
-
N-(2-hydrazino-2-oxoethyl)-4-(trifluoromethyl)benzamide
-
-
N-(2-hydrazino-2-oxoethyl)-4-nitrobenzamide
-
-
N-acetyl-L-cysteine
-
-
N-hydrazinocarbonylmethyl-2-nitrobenzamide
-
-
N-hydrazinocarbonylmethyl-2-trifluoromethylbenzamide
-
-
N-[4-(hydrazinosulfonyl)benzyl]acetamide
-
known to interact with the cofactor of PLP enzymes
NaF
-
10 mM, weak
O-acetylserine
-
strong
p-chloromercuribenzoate
p-hydroxymercuribenzoate
-
1 mM, 92% inhibition
phenylhydrazine
pyridoxal
-
12.5 mM, in presence of 12 mM L-cystine 12% inhibition, in presence of 1 mM L-cystine 37% inhibition
pyruvate
-
12.5 mM, 25% inhibition
rhizobitoxine
S-adenosylmethionine
-
weak
S-methyl-L-cysteine
-
substrate inhibition, when concentration exceeds 10 mM
Semicarbazide
-
-
Sodium cyanide
-
12.5 mM, 95% inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-keto-gamma-methiobutyrate
-
stimulates
alpha-keto-gamma-methiolbutyrate
-
-
alpha-ketoglutarate
-
activates
CYS3
a bZIP transcriptional activator, is the key regulator and necessary for regulation expression of the sulfur-related genes, binds on a consensus sequence for CYS3 binding
-
NaCl
NaCl considerably enhances the reaction rate at pH 6.8; NaCl considerably enhances the reaction rate at pH 6.8
pyridoxal
-
is 23% as effective as pyridoxal 5'-phosphate
TTHA1554
-
when an 8fold excess of TTHA1554 is added to TTHA1620, the cystathionine beta-lyase activity of TTHA1620 increases approximately 2fold
-
additional information
-
several other alpha-keto acids also stimulate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
2,4-dinitrophenyl-L-cysteine
0.013
3-hydroxy-kynurenine
-
-
0.6
beta-chloro-L-alanine
-
-
2.6
beta-chloroalanine
-
-
0.38 - 4.2
cystathionine
1
cystine
-
estimated from steady-state measurements
5.3
D-Cys
-
-
1 - 1.4
DL-homocysteine
0.4
kynurenine
-
-
0.04 - 15
L-cystathionine
0.29 - 4.8
L-cysteine
0.0812 - 5.4
L-cystine
0.033 - 3.6
L-djenkolate
4.5
L-homolanthionine
-
-
0.83
L-meso-lanthionine
-
-
32.8 - 34.2
L-methionine
0.35
L-selenocystathionine
-
-
2
L-selenodjenkolate
-
-
0.104
Lanthionine
-
-
6.5
O-methyl-DL-serine
-
-
0.008
pyridoxal phosphate
-
-
8.4
S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
-
23°C, pH 7.4
1.36 - 1.39
S-(1,2-dichlorovinyl)-L-cysteine
0.47
S-(2-aminoethyl)-L-Cys
-
-
0.01 - 1.66
S-(2-benzothiazolyl)-L-cysteine
1.02 - 1.78
S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
0.7
S-(p-bromophenyl)-L-cysteine
-
-
0.1 - 0.33
S-1,2-dichlorovinyl-L-cysteine
0.08
S-2,4-dinitrophenyl-L-cysteine
-
-
0.73
S-Benzyl-L-cysteine
-
-
0.67
S-methyl-L-Cys
-
-
2.3 - 5.3
S-methyl-L-cysteine
2.3
S-methyl-L-cysteine sulfoxide
-
-
4.6
Se-methyl-L-selenocysteine
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5
cystathionine
-
-
0.91 - 79
L-cystathionine
1.27
L-djenkolate
in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA, at 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00009 - 470
L-cystathionine