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Literature summary for 4.4.1.13 extracted from

  • Yamauchi, A.; Stijntjes, G.J.; Commandeur, J.N.M.; Vermeulen, N.P.E
    Purification of glutamine transaminase K/cysteine conjugate beta-lyase from rat renal cytosol based on hydrophobic interaction HPLC and gel permeation FPLC (1993), Protein Expr. Purif., 4, 552-562.
    View publication on PubMed
Search for more literature for 4.4.1.13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 S-1,2-dichlorovinyl-L-cysteine
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47400
-
 2 * 47400, SDS-PAGE Rattus norvegicus
100000
-
 GP-FPLC Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.47
-
 in absence of 2-oxo-4-methylthiobutyric acid Rattus norvegicus
6.4
-
 in presence of 2-oxo-4-methylthiobutyric acid Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-(1,2-dichloroethenyl)-L-cysteine + H2O
-
 Rattus norvegicus pyruvate + NH3 + dichlorovinyl mercaptane
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 47400, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
More rat kidney enzyme: identical with rat kidney cytosolic glutamine transaminase K Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus