Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bordetella avium |
General Stability | Organism |
---|---|
no decrease in activity after repeated freezing, -20°C, and thawing | Bordetella avium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | 10 mM, 19% inhibition | Bordetella avium | |
hydroxylamine | 1 mM, 92% inhibition | Bordetella avium | |
iodoacetamide | 10 mM, 61% inhibition | Bordetella avium | |
iodoacetate | 10 mM, 25% inhibition | Bordetella avium | |
KCN | 1 mM, 84% inhibition | Bordetella avium | |
L-canaline | 1 mM, 67% inhibition, 10 mM complete inhibition | Bordetella avium | |
NEM | 1 mM, 94% inhibition | Bordetella avium | |
p-hydroxymercuribenzoate | 1 mM, 92% inhibition | Bordetella avium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
L-djenkolate | - |
Bordetella avium | |
0.07 | - |
L-cystathionine | - |
Bordetella avium | |
0.104 | - |
Lanthionine | - |
Bordetella avium | |
0.47 | - |
S-(2-aminoethyl)-L-Cys | - |
Bordetella avium | |
0.67 | - |
S-methyl-L-Cys | - |
Bordetella avium | |
5.3 | - |
D-Cys | - |
Bordetella avium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Bordetella avium |
42606 | - |
2 * 42606, calculation from nucleotide sequence | Bordetella avium |
80000 | - |
gel filtration | Bordetella avium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella avium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bordetella avium |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-20°C, stable for 6 months | Bordetella avium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Cys + H2O | - |
Bordetella avium | ? | - |
? | |
L-cystathionine + H2O | - |
Bordetella avium | L-homocysteine + NH3 + pyruvate | - |
? | |
L-cystine + H2O | - |
Bordetella avium | L-thiocysteine + pyruvate + NH3 | - |
? | |
L-djenkolate + H2O | - |
Bordetella avium | pyruvate | - |
? | |
lanthionine + H2O | - |
Bordetella avium | L-cysteine + NH3 + pyruvate | - |
? | |
additional information | osteotoxin is lethal for MC3T3-E1 osteogenic cells, fetal bovine trabecular cells, UMR106-01(BSP) rat osteosarcoma cells and embryonic bovine tracheal cells. The cytotoxicity can be attributed to: 1. beta-cystathionase-catalyzed cleavage of L-cystine in the medium and formation of reactive sulfane-containing derivatives, 2. Transfer of sulfane sulfur to metabolically sensitive or structurally important proteins in the osteogenic cells | Bordetella avium | ? | - |
? | |
S-(2-aminoethyl)-L-Cys + H2O | - |
Bordetella avium | ? | - |
? | |
S-methyl-L-Cys + H2O | - |
Bordetella avium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Bordetella avium |
dimer | 2 * 42606, calculation from nucleotide sequence | Bordetella avium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
room temperature, t1/2: 24 min | Bordetella avium |
25 | 40 | stable, rapid decline in activity above 40°C | Bordetella avium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Bordetella avium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | each subunit of the dimer contains one molecule of pyridoxal 5'-phosphate | Bordetella avium |