Literature summary for 4.4.1.13 extracted from

Farsi, A.; Lodha, P.H.; Skanes, J.E.; Los, H.; Kalidindi, N.; Aitken, S.M.
Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase, and Saccharomyces cerevisiae cystathionine gamma-lyase and development of tools for the investigation of their mechanisms (2009), Biochem. Cell Biol., 87, 445-457.

Search for more literature for 4.4.1.13

Cloned(Commentary)

Cloned (Comment)	Organism
mutant enzymes are expressed in Escherichia coli KS1000 cells	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
F55D	the mutant shows 74fold reduced catalytic efficiency compared to the wild type enzyme	Escherichia coli
F55D/Y338E	the mutant shows 58000fold reduced catalytic efficiency compared to the wild type enzyme	Escherichia coli
Y338E	the mutant shows 2850fold reduced catalytic efficiency compared to the wild type enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Toyopearl butyl-650M resin column chromatography, and DEAE column chromatography (untagged enzyme), or Ni-NTA column chromatography (His-tagged enzyme)	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-cystathionine + H2O	-	Escherichia coli	L-homocysteine + NH3 + pyruvate	-	?

Synonyms

Synonyms	Comment	Organism
CBL	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Escherichia coli

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Release 2023.1 (January 2023)