Application | Comment | Organism |
---|---|---|
food industry | improvement of wine aroma during fermentation of a Vitis vinifera cultivar Sauvignon blanc juice | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain VIN 13 and in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
the buffer exchange conditions to stabilize the isolated protein and maintain its enzymatic activity include 500 mM KCl and 20 mM EDTA. By including 10% (w/v) glycerol in the buffer, less than 6% of the activity is lost in a freeze-thaw cycle, compared to the 56% loss in the presence of 10 mM imidazole | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.096 | - |
L-cystathionine | in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA, at 37°C | Saccharomyces cerevisiae | |
0.178 | - |
L-djenkolate | in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA, at 37°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, SDS-PAGE | Saccharomyces cerevisiae |
53000 | - |
4 * 53000, estimated from amino acid sequence | Saccharomyces cerevisiae |
195000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | Saccharomyces cerevisiae | 100% activity | L-homocysteine + NH3 + pyruvate | - |
? | |
L-cystathionine + H2O | Saccharomyces cerevisiae EC1118 | 100% activity | L-homocysteine + NH3 + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | E3VL26 | - |
- |
Saccharomyces cerevisiae EC1118 | E3VL26 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1258 | - |
using L-cystathionine as substrate,in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA, at 37°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-(4-methylpentan-2-one)-L-cysteine + H2O | - |
Saccharomyces cerevisiae | 4-mercapto-4-methylpentan-2-one + NH3 + pyruvate | Str3p is able to release 0.0123 mM 4-mercapto-4-methylpentan-2-one from 2 mM concentration of its precursor S-(2-methyl-4-oxopentan-2-yl)-L-cysteine | ? | |
L-cystathionine + H2O | 100% activity | Saccharomyces cerevisiae | L-homocysteine + NH3 + pyruvate | - |
? | |
L-cystathionine + H2O | 100% activity | Saccharomyces cerevisiae EC1118 | L-homocysteine + NH3 + pyruvate | - |
? | |
L-cysteine + H2O | 1.0% activity compared to L-cystathionine | Saccharomyces cerevisiae | ? | - |
? | |
L-cysteine + H2O | 1.0% activity compared to L-cystathionine | Saccharomyces cerevisiae EC1118 | ? | - |
? | |
L-cystine + H2O | 22% activity compared to L-cystathionine | Saccharomyces cerevisiae | ? | - |
? | |
L-djenkolate + H2O | most effective substrate, 154% activity compared to L-cystathionine | Saccharomyces cerevisiae | pyruvate + NH3 + ? | - |
? | |
additional information | no alpha-ketobutyrate is detected with any of the substrates susceptible for beta-lyase activity, confirming that Str3p has only beta-lyase activity. L-methionine is no substrate for Str3p | Saccharomyces cerevisiae | ? | - |
? | |
additional information | no alpha-ketobutyrate is detected with any of the substrates susceptible for beta-lyase activity, confirming that Str3p has only beta-lyase activity. L-methionine is no substrate for Str3p | Saccharomyces cerevisiae EC1118 | ? | - |
? | |
S-(1-hydroxyhexan-3-yl)-L-cysteine + H2O | - |
Saccharomyces cerevisiae | 3-mercaptohexan-1-ol + NH3 + pyruvate | Str3p is able to release 0.0021 mM 3-mercaptohexan-1-ol from 2 mM concentrations of its precursor (S)-3-(hexan-1-ol)-L-cysteine | ? | |
S-ethyl-L-cysteine | 9.0% activity compared to L-cystathionine | Saccharomyces cerevisiae | ? | - |
? | |
S-ethyl-L-cysteine | 9.0% activity compared to L-cystathionine | Saccharomyces cerevisiae EC1118 | ? | - |
? | |
S-methyl-L-cysteine + H2O | 7.4% activity compared to L-cystathionine | Saccharomyces cerevisiae | ? | - |
? | |
S-methyl-L-cysteine + H2O | 7.4% activity compared to L-cystathionine | Saccharomyces cerevisiae EC1118 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 52000, SDS-PAGE | Saccharomyces cerevisiae |
homotetramer | 4 * 53000, estimated from amino acid sequence | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
CBL | - |
Saccharomyces cerevisiae |
Str3p | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.91 | - |
L-cystathionine | in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA, at 37°C | Saccharomyces cerevisiae | |
1.27 | - |
L-djenkolate | in 50 mM phosphate buffer, pH 8.5, 0.02 mM pyridoxal 5'-phosphate, 1 mM EDTA, at 37°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
using L-cystathionine as substrate | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.5 | using L-cystathionine as substrate, no significant activity is observed below pH 7.0 or above pH 10.5 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Saccharomyces cerevisiae |
Organism | Comment | Expression |
---|---|---|
Saccharomyces cerevisiae | STR3 transcript levels in the modified strain VIN 13 are 18fold higher at day 5 but only 50% higher by the end of grape juice fermentation. In the VIN 13 control strain, the level of STR3 transcript under the control of its native promoter increase 12.3fold between day 5 and day 15 of fermentation | up |