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Literature summary for 4.4.1.13 extracted from

  • Adcock, H.J.; Brophy, P.M.; Teesdale-Spittle, P.H.; Buckberry, L.D.
    Purification and characterisation of a novel cysteine conjugate beta-lyase from the tapeworm Moniezia expansa (2000), Int. J. Parasitol., 30, 567-571.
    View publication on PubMed
Search for more literature for 4.4.1.13

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Monieza expansa 5829
-
mitochondrion
-
 Monieza expansa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 gel filtration Monieza expansa

Organism

Organism UniProt Comment Textmining
Monieza expansa
-
 tapeworm
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.037
-
-
 Monieza expansa

Storage Stability

Storage Stability Organism
-20°C, partially purified enzyme, 4 weeks, no loss of activity Monieza expansa
4°C, partially purified enzyme ,1 week, 41% loss of activity Monieza expansa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-keto-gamma-methiobutyric acid + L-aspartate transamination reaction Monieza expansa ?
-
 ?
S-(1,1,2-trichlorovinyl)-L-cysteine
-
 Monieza expansa ?
-
 ?
S-(E-1,2-Dichlorovinyl)-L-cysteine
-
 Monieza expansa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000-70000, SDS-PAGE Monieza expansa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Monieza expansa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
67
-
 5 min, complete loss of activity Monieza expansa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Monieza expansa