Information on EC 3.5.1.5 - urease

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.5.1.5
-
RECOMMENDED NAME
GeneOntology No.
urease
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
urea + H2O = CO2 + 2 NH3
show the reaction diagram
-
-
-
-
urea + H2O = CO2 + 2 NH3
show the reaction diagram
mechanism
-
urea + H2O = CO2 + 2 NH3
show the reaction diagram
active site contains three ionazable groups with pKa-values of 5.3, 6.6 and 9.0 that participate both in catalysis and substrate binding
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
carboxylic acid amide hydrolysis
-
-
condensation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
Atrazine degradation
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
Purine metabolism
-
urea degradation II
-
SYSTEMATIC NAME
IUBMB Comments
urea amidohydrolase
A nickel protein.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Arthritogenic cationic 19 kDa antigen
-
-
-
-
canatoxin
-
presence of a family of urease related genes in Canavalia ensiformis with at least three members: canatoxin, JBU and JBURE-II
embryo-specific soybean urease
-
-
JBU
Q8H6V8
presence of a family of urease related genes in Canavalia ensiformis with at least three members: canatoxin, JBU and JBURE-II
JBURE-II
Q8H6V8
-
JBURE-II
Q8H6V8
family of urease related genes in Canavalia ensiformis with at least three members: canatoxin, JBU and JBURE-II
Urea amidohydrolase
-
-
-
-
Urea amidohydrolase
-
-
Urea amidohydrolase
-
-
Urea amidohydrolase
-
-
Urea amidohydrolase
-
-
Urea amidohydrolase
Proteus vulgaris CCM 1956
-
-
-
Urea amidohydrolase
-
-
Urea amidohydrolase
Sporosarcina pasteurii CCM 2056
-
-
-
urease
-
-
urease
Q8FZW3
-
urease
Q8FZW3
-
-
urease
P07374
-
urease
P18314 and P18315 and P18316
-
urease
Enterobacter sp. R-SYB082
-
-
-
urease
Helicobacter hepaticus 3B1
-
-
-
urease
-
-
urease
Proteus vulgaris CCM 1956
-
-
-
urease
Sporosarcina pasteurii CCM 2056, Sporosarcina pasteurii CCM 2056T
-
-
-
urease
-
-
urease JBURE-IIb
E6Y5X0
-
CAS REGISTRY NUMBER
COMMENTARY
9002-13-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain WVU45
Uniprot
Manually annotated by BRENDA team
Actinomyces naeslundii WVU45
strain WVU45
Uniprot
Manually annotated by BRENDA team
Arthrobacter mobilis
-
-
-
Manually annotated by BRENDA team
bacterium strain SL100
gram-positive coccoid isolate prototype with an adhesin specific for gastric mucin
-
-
Manually annotated by BRENDA team
urease subunit beta 2; strain 1330, ATCC 23444
UniProt
Manually annotated by BRENDA team
urease subunit beta 2; strain 1330, ATCC 23444
UniProt
Manually annotated by BRENDA team
pigeonpea
-
-
Manually annotated by BRENDA team
commercial preparation
-
-
Manually annotated by BRENDA team
i.e. jack bean
-
-
Manually annotated by BRENDA team
subunits alpha, beta, gamma
P18314 and P18315 and P18316
UniProt
Manually annotated by BRENDA team
R-SYB082; strain R-SYB082
-
-
Manually annotated by BRENDA team
Enterobacter sp. R-SYB082
R-SYB082; strain R-SYB082
-
-
Manually annotated by BRENDA team
-
Q7XAC5
Uniprot
Manually annotated by BRENDA team
alpha-subunit
SwissProt
Manually annotated by BRENDA team
beta-subunit
SwissProt
Manually annotated by BRENDA team
enzyme activity does not contribute to acid resistance of strain ATCC 51449, enzyme is acid-independent. Supplementation of medium by nickel leads to tenfold increase in enzyme activity, independent of de novo protein synthesis
-
-
Manually annotated by BRENDA team
Helicobacter hepaticus 3B1
strain 3B1
-
-
Manually annotated by BRENDA team
ATCC 41653
-
-
Manually annotated by BRENDA team
ATCC 43504
-
-
Manually annotated by BRENDA team
enzyme is induced by acid. Supplementation of medium by nickel leads to tenfold increase in enzyme activity, independent of de novo protein synthesis
-
-
Manually annotated by BRENDA team
recombinantly expressed in Escherichia coli
Uniprot
Manually annotated by BRENDA team
Lactobacillus animalis MU-4
MU-4
-
-
Manually annotated by BRENDA team
strain IFO 14511
-
-
Manually annotated by BRENDA team
Lactobacillus fermentum IFO 14511
strain IFO 14511
-
-
Manually annotated by BRENDA team
strain Rt-5
-
-
Manually annotated by BRENDA team
Lactobacillus reuteri RT-5
strain Rt-5
-
-
Manually annotated by BRENDA team
strain PG-98
-
-
Manually annotated by BRENDA team
Lactobacillus ruminis PG-98
strain PG-98
-
-
Manually annotated by BRENDA team
CCM 1956
-
-
Manually annotated by BRENDA team
Proteus vulgaris CCM 1956
CCM 1956
-
-
Manually annotated by BRENDA team
strain E1F1
-
-
Manually annotated by BRENDA team
Rhodobacter capsulatus E1F1
strain E1F1
-
-
Manually annotated by BRENDA team
Sporosarcina pasteurii CCM 2056
CCM 2056
-
-
Manually annotated by BRENDA team
Sporosarcina pasteurii CCM 2056T
-
-
-
Manually annotated by BRENDA team
exhibits high urease activity and is capable of growing in urinary condition by forming robust biofilms
-
-
Manually annotated by BRENDA team
Streptococcus mitior
-
-
-
Manually annotated by BRENDA team
Streptococcus mitior
PG-118
-
-
Manually annotated by BRENDA team
Streptococcus mitior PG-118
PG-118
-
-
Manually annotated by BRENDA team
strain PG-202
-
-
Manually annotated by BRENDA team
Streptococcus salivarius PG-202
strain PG-202
-
-
Manually annotated by BRENDA team
strain TH3996
-
-
Manually annotated by BRENDA team
biovar 1A strain
-
-
Manually annotated by BRENDA team
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
slight stimulation
glycerol
-
optimum concentration: 15%
n-octylglucoside
-
optimum concentration: 0.1%
Ni2+
-
supplementation of growth medium by nickel leads to tenfold increase in enzyme activity, induction occuring at post-translational level
Ni2+
-
supplementation of growth medium by nickel leads to tenfold increase in enzyme activity
PEG
-
optimum concentration: 0.5%
SDS
-
optimum concentration: 0.02%
Triton X-100
-
optimum concentration: 0.05%
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.06
-
Urea
-
mutant H320N
0.068
-
Urea
-
mutant H320A
0.33
-
Urea
-
mutant H320Q
0.62
-
Urea
-
mutant R336Q
1.7
-
Urea
-
mutant D221A
60
-
Urea
-
mutant D221N
194
-
Urea
-
mutant H219A
322
-
Urea
-
mutant H219N
1860
-
Urea
-
mutant H219Q
2970
-
Urea
-
wild-type enzyme
3500
-
Urea
-
pH 7.0, 37C
5913
-
Urea
-
pH 7.0, 38C
additional information
-
additional information
-
pH-variation study on steady-state kinetic parameters
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1250
-
Urea
-
pH 7.0, 37C
17751
2000
-
Urea
-
pH 7.0, 38C
17751
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.017
-
(2S)-2-(dimethylamino)-3-(ethylthio)propan-1-ol
-
pH 8.2
0.018
-
(2S)-2-(dimethylamino)-3-(ethylthio)propan-1-ol
-
pH 5.0
0.002
-
(2S)-2-(dimethylamino)-3-ethoxypropane-1-thiol
-
pH 8.2
0.055
-
(2S)-2-(dimethylamino)-3-ethoxypropane-1-thiol
-
pH 5.0
0.002
-
(2S)-2-(dimethylamino)-3-mercaptopropan-1-ol
-
pH 8.2
0.013
-
(2S)-2-(dimethylamino)-3-mercaptopropan-1-ol
-
pH 5.0
0.002
-
(2S)-2-(dimethylamino)-3-methoxypropane-1-thiol
-
pH 8.2
0.019
-
(2S)-2-(dimethylamino)-3-methoxypropane-1-thiol
-
pH 5.0
0.34
-
(aminomethyl)methylphosphinic acid
-
pH 7.0, 37C
0.24
-
(aminomethyl)phosphonic acid
-
pH 7.0, 37C
0.314
-
(aminomethyl)phosphonic acid
-
pH 7.0, 37C
0.000407
-
(E)-1-((E)-3-phenylallylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.00017
-
(E)-1-(2-chlorobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.00091
-
(E)-1-(3,4,5-trimethoxybenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.00067
-
(E)-1-(3-bromobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000625
-
(E)-1-(3-chlorobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000127
-
(E)-1-(3-methoxybenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.00009
-
(E)-1-(3-nitrobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000122
-
(E)-1-(4-(N,N-dimethylamino)benzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.00077
-
(E)-1-(4-bromobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000398
-
(E)-1-(4-chlorobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000807
-
(E)-1-(4-methoxybenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000497
-
(E)-1-(4-nitrobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.00043
-
(E)-2-((E)-but-2-enylidene) hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.000127
-
(E)-2-(2,3-dihydroxybenzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.000152
-
(E)-2-(2-(benzyloxy)benzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.000191
-
(E)-2-(3-(benzyloxy)-4-methoxybenzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.000134
-
(E)-2-(4-hydroxy-3,5-dimethoxybenzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.000391
-
(E)-2-(4-hydroxybenzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.014
-
([[(benzyloxy)carbonyl]amino]methyl)methylphosphinothioic O-acid
-
-
0.0175
-
([[(benzyloxy)carbonyl]amino]methyl)methylphosphinothioic O-acid
-
-
0.000094
-
1,1-dimethyl-3-[(1S)-1-naphthalen-1-ylethyl]selenourea
-
-
0.358
-
1-benzyl-2-ethyl-6,6-dimethyl-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.106
-
1-benzyl-2-ethyl-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.754
-
1-benzyl-2-ethyl-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.000025
-
1-cyclohexyl-3,3-dimethyl-1-(1-methylethyl)selenourea
-
-
0.446
-
1-ethoxy-2-ethyl-6,6-dimethyl-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.566
-
1-ethoxy-2-ethyl-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.02
-
2,2'-bi-1,3,4-oxadiazole-5,5'(4H,4'H)-dithione
-
pH 5.0, 30C
0.03
-
2,2'-bi-1,3,4-oxadiazole-5,5'(4H,4'H)-dithione
-
pH 8.2, 30C
0.002
-
2,2'-bi-1,3,4-thiadiazole-5,5'(4H,4'H)-dithione
-
pH 5.0, 30C
0.005
-
2,2'-bi-1,3,4-thiadiazole-5,5'(4H,4'H)-dithione
-
pH 8.2, 30C
0.169
-
2-ethyl-1-(2-phenylethyl)-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.0293
-
2-ethyl-1-(4-methoxycyclohexyl)-6,6-dimethyl-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.0495
-
2-ethyl-1-(4-methoxyphenyl)-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.0817
-
2-ethyl-6-[2-(ethylsulfanyl)propyl]-1-(4-methoxyphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one
-
pH 3.85, 36C
0.0059
-
2-[(1E)-N-(allyloxy)butanimidoyl]-5,5-dimethylcyclohexane-1,3-dione
-
-
0.0086
-
2-[(1E)-N-ethoxybutanimidoyl]-5,5-dimethylcyclohexane-1,3-dione
-
-
0.000135
-
3-(4-bromobenzyl)-1,1-dimethylselenourea
-
-
0.000255
-
3-(4-bromophenyl)-1,1-dimethylthiourea
-
-
0.000124
-
3-dodecyl-1,1-dimethylthiourea
-
-
0.04
-
4-(4-benzoyl-5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)phenyl benzoate
-
pH 5.0, 30C
0.29
-
4-(4-benzoyl-5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)phenyl benzoate
-
pH 8.2, 30C
0.29
-
4-amino-5-(4-hydroxyphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 8.2, 30C
0.31
-
4-amino-5-(4-hydroxyphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 5.0, 30C
0.2
-
4-amino-5-pyridin-4-yl-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 8.2, 30C
0.24
-
4-amino-5-pyridin-4-yl-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 5.0, 30C
0.22
-
4-bromophenylboronic acid
-
pH 7.0, 37C
0.3
-
4-bromophenylboronic acid
-
pH 7.3, 27C
0.13
-
5-(1-naphthyl)-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C; pH 8.2, 30C
0.15
-
5-(3-hydroxyphenyl)-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, 30C
0.16
-
5-(3-hydroxyphenyl)-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C
0.05
-
5-(4-hydroxyphenyl)-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C; pH 8.2, 30C
0.002
-
5-(4-hydroxyphenyl)-1,3,4-thiadiazole-2(3H)-thione
-
pH 5.0, 30C; pH 8.2, 30C
0.06
-
5-(4-propoxyphenyl)-3-propyl-1,3,4-thiadiazole-2(3H)-thione
-
pH 5.0, 30C
0.19
-
5-(4-propoxyphenyl)-3-propyl-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, 30C
0.04
-
5-benzhydryl-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C
0.11
-
5-benzhydryl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, 30C
0.06
-
5-benzhydryl-1,3,4-thiadiazole-2(3H)-thione
-
pH 5.0, 30C
0.12
-
5-benzhydryl-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, 30C
0.14
-
5-benzyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C
0.17
-
5-benzyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, 30C
0.09
-
5-cyclohexyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C
0.1
-
5-cyclohexyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, 30C
0.16
-
5-phenyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, 30C
0.19
-
5-phenyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C
0.02
-
5-phenyl-1,3,4-thiadiazole-2(3H)-thione
-
pH 5.0, 30C
0.1
-
5-phenyl-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, 30C
0.09
-
5-pyridin-4-yl-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C; pH 8.2, 30C
0.11
-
5-pyridin-4-yl-1,3,4-thiadiazole-2(3H)-thione
-
pH 5.0, 30C; pH 8.2, 30C
0.005
-
5-[(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)methyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C; pH 8.2, 30C
0.005
-
5-[(5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)methyl]-1,3,4-thiadiazole-2(3H)-thione
-
pH 5.0, 30C
0.008
-
5-[(5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)methyl]-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, 30C
0.006
-
5-[3-(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)propyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C
0.01
-
5-[3-(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)propyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, 30C
0.09
-
5-[3-(benzyloxy)phenyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, 30C
0.16
-
5-[3-(benzyloxy)phenyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C
0.69
-
5-[4-(benzyloxy)phenyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 5.0, 30C
0.85
-
5-[4-(benzyloxy)phenyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, 30C
0.23
-
5-[4-(tert-butyl-dimethyl-silanyloxy)-phenyl]-3H-[1,3,4]oxadiazole-2-thione
-
pH 5.0, 30C; pH 8.2, 30C
0.15
-
5-[4-(tert-butyl-dimethyl-silanyloxy)-phenyl]-3H-[1,3,4]thiadiazole-2-thione
-
pH 5.0, 30C
0.25
-
5-[4-(tert-butyl-dimethyl-silanyloxy)-phenyl]-3H-[1,3,4]thiadiazole-2-thione
-
pH 8.2, 30C
0.77
-
Acetohydroxamic acid
Q8FZW3
competitive
0.34
-
aminomethyl(P-methyl)phosphinic acid
-
-
-
0.425
-
aminomethyl(P-methyl)phosphinic acid
-
-
-
0.2
-
Boric acid
-
pH 7.0, 37C
0.35
-
Boric acid
-
pH 7.3, 27C
1.5
-
butylboronic acid
-
pH 7.0, 37C
1.8
-
butylboronic acid
-
pH 7.3, 27C
1.04
-
Hydroxyurea
Q8FZW3
competitive
0.0054
-
methyl (7E)-7-[4,4-dimethyl-3-(3-methylisoxazol-5-yl)-2,6-dioxocyclohexyl]-7-(ethoxyimino)heptanoate
-
-
0.0049
-
methyl 5-[(1E)-N-(allyloxy)butanimidoyl]-2,2-dimethyl-4,6-dioxocyclohexanecarboxylate
-
-
0.0027
-
methyl 5-[(1E)-N-ethoxybutanimidoyl]-2,2-dimethyl-4,6-dioxocyclohexanecarboxylate
-
pH 4.95, 36C
0.0041
-
methyl 5-[(1E)-N-ethoxyethanimidoyl]-2,2-dimethyl-4,6-dioxocyclohexanecarboxylate
-
-
0.018
-
methyl[(methylamino)methyl]phosphinic acid
-
-
0.018
-
methyl[(methylamino)methyl]phosphinic acid
-
pH 7.0, 37C
0.027
-
methyl[(methylamino)methyl]phosphinic acid
-
-
0.224
-
methyl[(methylamino)methyl]phosphinic acid
-
pH 7.0, 37C
0.00017
-
N-(N'-benzyloxycarbonylglycyl)aminomethyl(P-methyl)phosphinothioic acid
-
-
0.00045
-
N-(N'-benzyloxycarbonylglycyl)aminomethyl(P-methyl)phosphinothioic acid
-
-
0.043
-
N-benzyloxycarbonylaminomethyl(P-methyl)phosphinic acid
-
-
0.065
-
N-benzyloxycarbonylaminomethyl(P-methyl)phosphinic acid
-
-
0.002
-
N-dimethyl-L-cysteine
-
pH 8.2
0.009
-
N-dimethyl-L-cysteine
-
pH 5.0
0.006
-
N-dimethyl-S-methyl-L-cysteine
-
pH 5.0
0.008
-
N-dimethyl-S-methyl-L-cysteine
-
pH 8.2
7
-
N-ethylmaleimide
-
Ki(fast), pH 6.4
7.5
-
N-ethylmaleimide
-
Ki(slow), pH 6.4
0.021
-
N-glycyl-aminomethyl(P-methyl)phosphinic acid
-
-
0.03
-
N-glycyl-aminomethyl(P-methyl)phosphinic acid
-
-
0.176
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-phenylalaninamide
-
-
0.215
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-phenylalaninamide
-
-
0.037
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide
-
-
0.041
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide
-
-
0.12
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-valinamide
-
-
0.208
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-valinamide
-
-
0.135
-
N2-[(benzyloxy)carbonyl]-N-[[hydroxy(methyl)phosphoryl]methyl]glycinamide
-
-
0.178
-
N2-[(benzyloxy)carbonyl]-N-[[hydroxy(methyl)phosphoryl]methyl]glycinamide
-
-
1.3
-
NaF
-
pH 7.0, 37C
0.025
-
O-benzyl-N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide
-
-
0.0326
-
O-benzyl-N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide
-
-
2
-
Phenylboronic acid
-
pH 7.0, 37C
2.5
-
Phenylboronic acid
-
pH 7.3, 27C
0.011
-
S-ethyl-L-cysteine
-
pH 5.0
0.014
-
S-ethyl-L-cysteine
-
pH 8.2
0.0024
-
tetrachloro-o-benzoquinone
-
-
0.00045
-
tetrachloro-p-benzoquinone
-
-
0.0196
-
Thiourea
-
pH 8.2, 37C
26.12
-
Thiourea
Q8FZW3
competitive
0.095
-
[(acetylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.295
-
[(acetylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.148
-
[(acetylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.072
-
[(benzylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.571
-
[(benzylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.00062
-
[(dimethylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.0037
-
[(dimethylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.013
-
[(dimethylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.021
-
[(dimethylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.07
-
[(methylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.204
-
[(methylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.115
-
[[benzyl(methyl)amino]methyl]phosphonic acid
-
pH 7.0, 37C
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.1
-
(2-[[(2-[[2-(hydroxy-kappaO)ethyl]amino-kappaN]ethyl)imino-kappaN]methyl]-4-nitrophenolato-kappaO)(methoxymethanolato-kappaO)zinc
-
value above 0.1, pH and temperature not specified in the publication
0.0224
-
(2-[[(2-[[2-(hydroxy-kappaO)ethyl]amino-kappaN]ethyl)imino-kappaN]methyl]-4-nitrophenolato-kappaO)(nitrato-kappaO)copper
-
pH and temperature not specified in the publication
0.03251
-
(2E)-3-(1,3-benzodioxol-5-yl)-1-(2-hydroxyphenyl)prop-2-en-1-one
-
-
1.1
-
(aminomethyl)methylphosphinic acid
-
pH 7.0, 37C
0.432
-
(aminomethyl)phosphonic acid
-
pH 7.0, 37C
0.7
-
(aminomethyl)phosphonic acid
-
pH 7.0, 37C
0.000457
-
(E)-1-((E)-3-phenylallylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000215
-
(E)-1-(2-chlorobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000989
-
(E)-1-(3,4,5-trimethoxybenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000767
-
(E)-1-(3-bromobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.00067
-
(E)-1-(3-chlorobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000177
-
(E)-1-(3-methoxybenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000102
-
(E)-1-(3-nitrobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000127
-
(E)-1-(4-(N,N-dimethylamino)benzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000707
-
(E)-1-(4-bromobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.00041
-
(E)-1-(4-chlorobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000887
-
(E)-1-(4-methoxybenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000547
-
(E)-1-(4-nitrobenzylidene)thiosemicarbazide
-
pH 8.2, 37C
-
0.000746
-
(E)-2-((E)-but-2-enylidene) hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.00022
-
(E)-2-(2,3-dihydroxybenzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.000263
-
(E)-2-(2-(benzyloxy)benzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.00033
-
(E)-2-(3-(benzyloxy)-4-methoxybenzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.000233
-
(E)-2-(4-hydroxy-3,5-dimethoxybenzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.000682
-
(E)-2-(4-hydroxybenzylidene)hydrazinecarbothioamide
-
pH 8.2, 37C
-
0.002
0.003
(Hg2)2+
-
-
0.112
-
([[(benzyloxy)carbonyl]amino]methyl)methylphosphinothioic O-acid
-
-
0.158
-
([[(benzyloxy)carbonyl]amino]methyl)methylphosphinothioic O-acid
-
-
0.03
-
1,4-Naphthoquinone
-
pH 7.7, temperature not provided
0.125
-
2,2'-bi-1,3,4-oxadiazole-5,5'(4H,4'H)-dithione
-
pH 8.2, IC50: below 0.125 mM
0.133
-
2,2'-bi-1,3,4-oxadiazole-5,5'(4H,4'H)-dithione
-
pH 8.2, IC50: 0.133 mM
0.5
-
2,2'-bi-1,3,4-thiadiazole-5,5'(4H,4'H)-dithione
-
pH 8.2, IC50: 0.5 mM
0.18
-
2,2'-thenoin
-
IC50: 0.18 mM
0.138
-
2,4,6-trihydroxy-9H-xanthen-9-one
-
pH 6.8, 30C
0.02631
-
2,4-diphenyl-2,3-dihydro-1,5-benzothiazepine
-
-
0.02465
-
2-(4-methylphenyl)-4-phenyl-2,3-dihydro-1,5-benzothiazepine
-
-
0.03671
-
2-(4-nitrophenyl)-4-phenyl-2,3-dihydro-1,5-benzothiazepine
-
-
0.47
-
2-hydroxy-1-phenylethanone
-
IC50: 0.47 mM
0.09374
-
2-[(2S,4R)-2-(4-fluorophenyl)-2,3,4,5-tetrahydro-1,5-benzothiazepin-4-yl]phenol
-
-
0.09121
-
2-[(2S,4R)-2-phenyl-2,3,4,5-tetrahydro-1,5-benzothiazepin-4-yl]phenol
-
-
0.01907
-
2-[2-(1,3-benzodioxol-5-yl)-2,3-dihydro-1,5-benzothiazepin-4-yl]phenol
-
-
0.07385
-
3,3'-(2-bromobenzylidene)-bis-(4-hydroxycoumarin)
-
-
0.08453
-
3,3'-(2-chlorobenzylidene)-bis-(4-hydroxycoumarin)
-
-
0.05218
-
3,3'-(2-phenylpropylidene)-bis-(4-hydroxycoumarin)
-
-
0.03503
-
3,3'-(2-pyridyl-methylene)-bis-(4-hydroxycoumarin)
-
-
0.07293
-
3,3'-(3-chlorobenzylidene)-bis-(4-hydroxycoumarin)
-
-
0.05906
-
3,3'-(3-cinnamylidene)-bis-(4-hydroxycoumarin)
-
-
0.07191
-
3,3'-(3-hydroxybenzylidene)-bis-(4-hydroxycoumarin)
-
-
0.04841
-
3,3'-(3-indolidene)-bis-(4-hydroxycoumarin)
-
-
0.07368
-
3,3'-(4-hydroxybenzylidene)-bis-(4-hydroxycoumarin)
-
-
0.0553
-
3,3'-(4-methoxybenzylidene)-bis-(4-hydroxycoumarin)
-
-
0.06501
-
3,3'-(4-nitrobenzylidene)-bis-(4-hydroxycoumarin)
-
-
0.04292
-
3,3'-(crotonalidene)-bis-(4-hydroxycoumarin)
-
-
0.01501
-
3,3'-methylenebis-(4-hydroxycoumarin)
-
-
0.06639
-
3,3'-n-butylidene-bis(4-hydroxycoumarin)
-
-
0.08632
-
3,3'-n-hexylidene-bis(4-hydroxycoumarin)
-
-
0.037
-
3,4,5-trihydroxy-9H-xanthen-9-one
-
pH 6.8, 30C
0.000025
-
3,5-dimethylphenyl diamidophosphate
-
-
0.011
-
4-(1-amino-2-(4-hydroxyphenyl)ethyl)benzene-1,2,3-triol
-
-
0.047
-
4-(1-amino-2-(4-hydroxyphenyl)ethyl)benzene-1,2-diol
-
-
0.086
-
4-(2,3-dimethylphenyl)-5-phenyl-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 6.8, 30C
-
0.0456
-
4-(2,4-dimethylphenyl)-5-(3-nitrophenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 6.8, 30C
-
0.234
-
4-(4-benzoyl-5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)phenyl benzoate
-
pH 8.2, IC50: 0.234 mM
0.032
-
4-(4-bromophenyl)-5-[hydroxy(phenyl)methyl]-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH and temperature not specified in the publlication
0.0182
-
4-(4-chlorophenyl)-5-[hydroxy(phenyl)methyl]-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH and temperature not specified in the publlication
0.000016
-
4-(benzyloxy)phenyl diamidophosphate
-
-
0.03
-
4-(p-hydroxyphenethyl)pyrogallol
-
-
0.125
-
4-amino-5-(4-hydroxyphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 8.2, IC50: below 0.125 mM
0.163
-
4-amino-5-pyridin-4-yl-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 8.2, IC50: 0.163 mM
0.079
-
4-butylbenzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0079
-
4-butylbenzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.124
-
4-decylbenzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.168
-
4-dodecylbenzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.034
-
4-ethylbenzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0049
-
4-ethylbenzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.091
-
4-hexylbenzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0123
-
4-hexylbenzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.000063
-
4-nitrophenyl diamidophosphate
-
-
0.103
-
4-octylbenzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.058
-
4-octylbenzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.25
-
4-tetradecylbenzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.069
-
4-[2-(3,4-diethoxyphenyl)ethyl]benzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.071
-
4-[2-(3,4-dimethoxyphenyl)ethyl]benzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0227
-
4-[2-(3,4-dimethoxyphenyl)ethyl]benzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.204
-
4-[2-(3,4-dimethoxyphenyl)ethyl]phenol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.052
-
4-[2-(3-bromophenyl)ethyl]benzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0169
-
4-[2-(3-bromophenyl)ethyl]benzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.235
-
4-[2-(3-chlorophenyl)ethyl]-1,2-dimethoxybenzene
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.05
-
4-[2-(3-chlorophenyl)ethyl]benzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0157
-
4-[2-(3-chlorophenyl)ethyl]benzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.048
-
4-[2-(4-bromophenyl)ethyl]benzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.254
-
4-[2-(4-chlorophenyl)ethyl]-1,2-dimethoxybenzene
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.047
-
4-[2-(4-chlorophenyl)ethyl]benzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0083
-
4-[2-(4-chlorophenyl)ethyl]benzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.043
-
4-[2-(4-fluorophenyl)ethyl]benzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0062
-
4-[2-(4-fluorophenyl)ethyl]benzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.032
-
4-[2-(4-hydroxyphenyl)ethyl]benzene-1,2,3-triol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0015
-
4-[2-(4-hydroxyphenyl)ethyl]benzene-1,2-diol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.125
-
5-(1-naphthyl)-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: below 0.125 mM
0.214
-
5-(1-naphthyl)-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.214 mM
0.3132
-
5-(2-chlorophenyl)-N-(2,6-dimethylphenyl)-1,3,4-thiadiazol-2-amine
-
pH 6.8, 30C
-
0.4596
-
5-(3-chlorophenyl)-4-(2,4-dimethylphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 6.8, 30C
-
0.357
-
5-(3-chlorophenyl)-4-(2,6-dimethylphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 6.8, 30C
-
0.125
-
5-(3-hydroxyphenyl)-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: below 0.125 mM
0.1708
-
5-(4-chlorophenyl)-4-(2,4-dimethylphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH 6.8, 30C
-
0.4836
-
5-(4-chlorophenyl)-N-(2,3-dimethylphenyl)-1,3,4-thiadiazol-2-amine
-
pH 6.8, 30C
-
0.2147
-
5-(4-chlorophenyl)-N-(2,6-dimethylphenyl)-1,3,4-thiadiazol-2-amine
-
pH 6.8, 30C
-
0.5
-
5-(4-hydroxyphenyl)-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.5 mM
0.25
-
5-benzhydryl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.25 mM
0.95
-
5-benzhydryl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.95 mM
0.125
-
5-benzhydryl-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, IC50: below 0.125 mM
0.125
-
5-benzyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: below 0.125 mM
0.124
-
5-cyclohexyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.124 mM
0.125
-
5-cyclohexyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.125 mM
0.025
-
5-hydroxy-1,4-naphthoquinone
-
pH 7.7, temperature not provided
0.125
-
5-phenyl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: below 0.125 mM
0.25
-
5-phenyl-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.25 mM
0.29
-
5-phenyl-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.29 mM
0.125
-
5-pyridin-4-yl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: below 0.125 mM
0.2
-
5-pyridin-4-yl-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: above 0.2 mM
0.124
-
5-pyridin-4-yl-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.124 mM
0.125
-
5-pyridin-4-yl-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, IC50: below 0.125 mM
0.095
-
5-[(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)methyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.095 mM
0.5
-
5-[(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)methyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.5 mM
0.095
-
5-[(5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)methyl]-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.095 mM
0.5
-
5-[(5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)methyl]-1,3,4-thiadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.5 mM
0.093
-
5-[3-(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)propyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.093 mM
0.125
-
5-[3-(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)propyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: below 0.125 mM
0.41
-
5-[4-(benzyloxy)phenyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.41 mM
0.5
-
5-[4-(benzyloxy)phenyl]-1,3,4-oxadiazole-2(3H)-thione
-
pH 8.2, IC50: 0.5 mM
0.244
-
5-[4-(tert-butyl-dimethyl-silanyloxy)-phenyl]-3H-[1,3,4]oxadiazole-2-thione
-
pH 8.2, IC50: 0.244 mM
0.0167
-
5-[hydroxy(phenyl)methyl]-4-(4-methylphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH and temperature not specified in the publlication
0.1317
-
5-[hydroxy(phenyl)methyl]-4-(4-nitrophenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione
-
pH and temperature not specified in the publlication
0.01506
-
7'-hydroxy-2,2'-dioxo-2H,2'H-6,8'-bichromen-7-yl alpha-L-mannopyranoside
-
-
0.09367
-
7'-hydroxy-2,2'-dioxo-2H,2'H-6,8'-bichromen-7-yl alpha-L-mannopyranoside
-
-
0.02417
-
7'-hydroxy-2,2'-dioxo-3'-[(2-oxo-2H-chromen-7-yl)oxy]-2H,2'H-8,8'-bichromen-7-yl alpha-D-glucopyranoside
-
-
0.02939
-
7'-hydroxy-2,2'-dioxo-3'-[(2-oxo-2H-chromen-7-yl)oxy]-2H,2'H-8,8'-bichromen-7-yl alpha-D-glucopyranoside
-
-
0.03762
-
7'-methoxy-2,2'-dioxo-3'-[(2-oxo-2H-chromen-7-yl)oxy]-2H,2'H-8,8'-bichromen-7-yl alpha-D-glucopyranoside
-
-
0.04
-
7'-methoxy-2,2'-dioxo-3'-[(2-oxo-2H-chromen-7-yl)oxy]-2H,2'H-8,8'-bichromen-7-yl alpha-D-glucopyranoside
-
-
0.14
-
7,8,4'-trihydroxyisoflavone
-
-
0.02205
-
7-hydroxy-2,2'-dioxo-2H,2'H-6,8'-bichromen-7'-yl alpha-L-mannopyranoside
-
-
0.0263
-
7-hydroxy-2,2'-dioxo-2H,2'H-6,8'-bichromen-7'-yl alpha-L-mannopyranoside
-
-
0.05527
-
7-[(7-methoxy-2-oxo-2H-chromen-3-yl)oxy]-2-oxo-2H-chromen-6-yl alpha-D-glucopyranoside
-
-
0.06191
-
7-[(7-methoxy-2-oxo-2H-chromen-3-yl)oxy]-2-oxo-2H-chromen-6-yl alpha-D-glucopyranoside
-
-
0.02901
-
8-hydroxy-3-[(6-hydroxy-2-oxo-2H-chromen-7-yl)oxy]-2-oxo-2H-chromen-7-yl beta-D-glucopyranoside
-
-
0.0316
-
8-hydroxy-3-[(6-hydroxy-2-oxo-2H-chromen-7-yl)oxy]-2-oxo-2H-chromen-7-yl beta-D-glucopyranoside
-
-
0.005
-
Acetohydroxamic acid
-
IC50: 0.005 mM
0.017
-
Acetohydroxamic acid
-
-
0.02221
-
Acetohydroxamic acid
-
pH 8.2, 30C
0.0241
-
Acetohydroxamic acid
-
pH 8.2, 30C
0.045
-
Acetohydroxamic acid
-
pH and temperature not specified in the publication
2.9
-
acetol
-
IC50: 2.9 mM
0.000000023
-
Ag+
-
pH 7.0, 37C
0.002
0.003
Ag+
-
-
1.1
-
aminomethyl(P-methyl)phosphinic acid
-
-
-
2.53
-
aminomethyl(P-methyl)phosphinic acid
-
-
-
0.14
-
avicularin
-
pH 6.0, 37C
0.031
-
beta-(o-methoxyphenyl)-gamma,gamma-bis(8-quinolinoxy)germa-gamma-lactone
-
-
0.02425
-
chloro(2-[[(2-[[2-(hydroxy-kappaO)ethyl]amino-kappaN]ethyl)imino-kappaN]methyl]-4-nitrophenolato-kappaO)copper
-
pH and temperature not specified in the publication
0.002
0.003
Cu2+
-
-
0.0033
-
Cu2+
-
pH 7.0, 37C
0.36
-
furoin
-
IC50: 0.36 mM
0.12
-
guaijaverin
-
pH 6.0, 37C
0.000071
-
Hg2+
-
pH 7.0, 37C
0.002
0.003
Hg2+
-
-
0.1
-
Hydroxyurea
-
IC50: 0.1 mM
0.16
-
isoquercitrin
-
pH 6.0, 37C
0.02801
-
methyl 2-amino-4,5,6,7,8,9-hexahydrocycloocta[b]thiophene-3-carboxylate
-
pH 8.2, 30C
0.03184
-
methyl 2-amino-4,5,6,7,8,9-hexahydrocycloocta[b]thiophene-3-carboxylate
-
pH 8.2, 30C
0.00534
-
methyl 2-amino-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.0093
-
methyl 2-amino-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.00083
-
methyl 2-amino-4-methylthiophene-3-carboxylate
-
pH 8.2, 30C
0.00281
-
methyl 2-amino-4-methylthiophene-3-carboxylate
-
pH 8.2, 30C
0.01107
-
methyl 2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate
-
pH 8.2, 30C
0.01658
-
methyl 2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate
-
pH 8.2, 30C
0.00213
-
methyl 2-amino-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carboxylate
-
pH 8.2, 30C
0.00504
-
methyl 2-amino-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carboxylate
-
pH 8.2, 30C
0.01832
-
methyl 2-[(methoxycarbonyl)amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.02495
-
methyl 2-[(methoxycarbonyl)amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.00077
-
methyl 2-[bis(chlorocarbonyl)amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.00299
-
methyl 2-[bis(chlorocarbonyl)amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.00635
-
methyl 2-[[(chloromethoxy)carbonyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.01242
-
methyl 2-[[(chloromethoxy)carbonyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.0271
-
methyl 2-[[(chloromethoxy)carbonyl]amino]-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate
-
pH 8.2, 30C
0.02908
-
methyl 2-[[(chloromethoxy)carbonyl]amino]-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate
-
pH 8.2, 30C
0.01321
-
methyl 2-[[(dichloromethoxy)carbonyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.01945
-
methyl 2-[[(dichloromethoxy)carbonyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.01054
-
methyl 2-[[(methylsulfanyl)carbonothioyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.01369
-
methyl 2-[[(methylsulfanyl)carbonothioyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate
-
pH 8.2, 30C
0.06
-
methyl[(methylamino)methyl]phosphinic acid
-
-
0.06
-
methyl[(methylamino)methyl]phosphinic acid
-
pH 7.0, 37C
0.153
-
methyl[(methylamino)methyl]phosphinic acid
-
-
0.884
-
methyl[(methylamino)methyl]phosphinic acid
-
pH 7.0, 37C
0.4261
-
N-(2,3-dimethylphenyl)-5-phenyl-1,3,4-thiadiazol-2-amine
-
pH 6.8, 30C
-
0.3982
-
N-(2,4-dimethylphenyl)-5-(3-nitrophenyl)-1,3,4-thiadiazol-2-amine
-
pH 6.8, 30C
-
0.00002
-
N-(2-methoxy-4-nitrophenyl)phosphoric triamide
-
-
0.003
-
N-(2-nitrophenyl)phosphoric triamide
-
-
0.000014
-
N-(2-phenylethyl)phosphoric triamide
-
-
0.01386
-
N-(2-phenylethyl)phosphorothioic triamide
-
-
0.00557
-
N-(3-methoxyphenyl)phosphoric triamide
-
-
0.04255
-
N-(3-methylpyridin-2-yl)phosphorothioic triamide
-
-
0.00071
-
N-(3-morpholin-2-ylpropyl)phosphoric triamide
-
-
0.00007
-
N-(3-nitropyridin-2-yl)phosphoric triamide
-
-
0.04425
-
N-(4-cyclohexylphenyl)phosphoric triamide
-
-
0.00001
-
N-(4-methoxy-1,3-benzothiazol-2-yl)phosphoric triamide
-
-
0.00001
-
N-(4-methyl-1,3-benzothiazol-2-yl)phosphoric triamide
-
-
0.000003
-
N-(4-methyl-2-nitrophenyl)phosphoric triamide
-
-
0.0003
-
N-(4-nitrophenyl)phosphoric triamide
-
-
0.01298
-
N-(4-phenoxyphenyl)phosphoric triamide
-
-
0.000005
-
N-(6-ethoxy-1,3-benzothiazol-2-yl)phosphoric triamide
-
-
0.000005
-
N-(6-fluoro-1,3-benzothiazol-2-yl)phosphoric triamide
-
-
0.0018
-
N-(N'-benzyloxycarbonylglycyl)aminomethyl(P-methyl)phosphinothioic acid
-
-
0.0031
-
N-(N'-benzyloxycarbonylglycyl)aminomethyl(P-methyl)phosphinothioic acid
-
-
0.000002
-
N-1,3-benzothiazol-2-ylphosphoric triamide
-
-
0.00296
-
N-1,3-benzothiazol-2-ylphosphorothioic triamide
-
-
0.00256
-
N-adamant-1-ylphosphoric triamide
-
-
0.123
-
N-benzyloxycarbonylaminomethyl(P-methyl)phosphinic acid
-
-
0.319
-
N-benzyloxycarbonylaminomethyl(P-methyl)phosphinic acid
-
-
0.06
-
N-glycyl-aminomethyl(P-methyl)phosphinic acid
-
-
0.086
-
N-glycyl-aminomethyl(P-methyl)phosphinic acid
-
-
0.0172
-
N-hydroxyacetamide
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
0.0001
-
N-n-butylthiophosphorictriamide
-
-
0.00053
-
N-naphthalen-1-ylphosphoric triamide
-
-
0.00363
-
N-phenylphosphoric triamide
-
-
0.000048
-
N-[2-(4-fluorophenyl)ethyl]phosphoric triamide
-
-
0.00002
-
N-[2-(difluoromethoxy)phenyl]phosphoric triamide
-
-
0.075
-
N-[2-(trifluoromethoxy)phenyl]phosphoric triamide
-
-
0.0035
-
N-[4-(benzyloxy)phenyl]phosphoric triamide
-
-
0.6
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-phenylalaninamide
-
-
0.754
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-phenylalaninamide
-
-
0.15
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide
-
-
0.342
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide
-
-
0.485
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-valinamide
-
-
0.617
-
N-[[hydroxy(methyl)phosphoryl]methyl]-L-valinamide
-
-
0.45
-
N2-[(benzyloxy)carbonyl]-N-[[hydroxy(methyl)phosphoryl]methyl]glycinamide
-
-
0.64
-
N2-[(benzyloxy)carbonyl]-N-[[hydroxy(methyl)phosphoryl]methyl]glycinamide
-
-
0.08
-
O-benzyl-N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide
-
-
0.183
-
O-benzyl-N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide
-
-
0.01654
-
O-[4-(benzyloxy)phenyl] diamidothiophosphate
-
-
0.0051
-
p-benzoquinone
-
-
0.000003
-
phenylphosphorodiamidate
-
-
0.08
-
quercetin
-
pH 6.0, 37C
0.19
-
quercetin-4-O-beta-D-glucopyranoside
-
pH 6.0, 37C
-
0.2
-
quercitrin
-
pH 6.0, 37C
0.0209
-
tetracosyl (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate
-
pH 6.8, 30C
0.01506
-
Thiourea
-
-
0.021
-
Thiourea
-
pH 6.8, 30C
0.021
-
Thiourea
-
pH and temperature not specified in the publlication
0.021
-
Thiourea
-
pH 6.8, 30C
0.021
-
Thiourea
-
pH 8.2, 37C
0.0211
-
Thiourea
-
-
0.258
-
[(acetylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
1.455
-
[(acetylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.463
-
[(acetylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.256
-
[(benzylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
1.632
-
[(benzylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.0038
-
[(dimethylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.0144
-
[(dimethylamino)methyl]methylphosphinic acid
-
pH 7.0, 37C
0.049
-
[(dimethylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.082
-
[(dimethylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.228
-
[(methylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.678
-
[(methylamino)methyl]phosphonic acid
-
pH 7.0, 37C
0.0161
-
[5-[(4-methylphenyl)amino]-1,3,4-oxadiazol-2-yl](phenyl)methanol
-
pH and temperature not specified in the publlication
0.0803
-
[5-[(4-nitrophenyl)amino]-1,3,4-oxadiazol-2-yl](phenyl)methanol
-
pH and temperature not specified in the publlication
0.3
-
[[benzyl(methyl)amino]methyl]phosphonic acid
-
pH 7.0, 37C
0.227
-
2-[2-(3,4-dimethoxyphenyl)ethyl]phenol
-
cell free urease preparation, pH not specified in the publication, temperature not specified in the publication
additional information
-
3,3'-(1H-indol-3-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.21
additional information
-
3,3'-(1H-pyrrol-2-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.33
0.08181
-
3,3'-(2-methoxybenzylidene)-bis-(4-hydroxycoumarin)
-
-
additional information
-
3,3'-(2-phenylpropane-1,1-diyl)bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.28
0.04609
-
3,3'-(2-pyrolidene)-bis-(4-hydroxycoumarin)
-
-
additional information
-
3,3'-(2E)-but-2-ene-1,1-diylbis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.36
0.05978
-
3,3'-(3-methoxybenzylidene)-bis-(4-hydroxycoumarin)
-
-
additional information
-
3,3'-(3-phenylpropane-1,1-diyl)bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.17
0.06728
-
3,3'-(dihydrocinnamylidene)-bis-(4-hydroxycoumarin)
-
-
additional information
-
3,3'-(naphthalen-1-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.07
additional information
-
3,3'-(pyridin-3-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.45
additional information
-
3,3'-(pyridin-4-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.23
additional information
-
3,3'-butane-1,1-diylbis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.17
additional information
-
3,3'-ethane-1,1-diylbis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.20
0.0613
-
3,3'-ethylidenebis-(4-hydroxycoumarin)
-
-
additional information
-
3,3'-hexane-1,1-diylbis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.06
additional information
-
3,3'-methanediylbis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.82
0.08474
-
3,3'-n-pentylidene-bis(4-hydroxycoumarin)
-
-
additional information
-
3,3'-pentane-1,1-diylbis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.19
additional information
-
3,3'-[(1E)-1-phenylprop-1-ene-3,3-diyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.22
additional information
-
3,3'-[(2-bromophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.13
additional information
-
3,3'-[(2-chlorophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.07
additional information
-
3,3'-[(2-methoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.08
additional information
-
3,3'-[(3,4,5-trimethoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.08
additional information
-
3,3'-[(3,4-dimethoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.10
additional information
-
3,3'-[(3-aminophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.12
additional information
-
3,3'-[(3-chlorophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.13
additional information
-
3,3'-[(3-ethoxy-4-hydroxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.04
additional information
-
3,3'-[(3-hydroxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.14
additional information
-
3,3'-[(3-methoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.25
additional information
-
3,3'-[(3-nitrophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.09
additional information
-
3,3'-[(4-hydroxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.13
additional information
-
3,3'-[(4-methoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.22
additional information
-
3,3'-[(4-nitrophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.15
additional information
-
3,3'-[[4-(1-methylethyl)phenyl]methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.08
additional information
-
3,3'-[[4-(dimethylamino)phenyl]methanediyl]bis(4-hydroxy-2H-chromen-2-one)
-
pIC50: 4.09
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10
45
Q8FZW3
-
20
60
-
20C: about 90% of maximal activity, 60C: about 40% of maximal activity, soluble enzyme
25
75
-
25C: about 80% of maximal activity, 75C: about 45% of maximal activity
30
70
-
30C: about 40% of maximal activity, 70C: about 10% of maximal activity
40
70
-
40C: about 50% of maximal activity, 70C: about 80% of maximal activity, agar-immobilized enzyme
40
80
-
about 60% of maximal activity at 40C and at 80C
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
-
Q8FZW3
-
5.6
-
E6Y5X0
calculated
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Rhodobacter capsulatus E1F1
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Brucella abortus (strain 2308)
Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198)
Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198)
Helicobacter pylori (strain ATCC 700392 / 26695)
Helicobacter pylori (strain ATCC 700392 / 26695)
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
urease is crystallized and the resulting crystals are diffracted to 2.05 A resolution using synchrotron radiation. The crystals belong to the hexagonal space group P6(3)322, with unit-cell parameters a = b = 138.57, c = 198.36 A
-
urease is crystallized at 2.05 A resolution. The active-site architecture of JBU is similar to that of bacterial ureases containing a bi-nickel center. JBU has a bound phosphate and covalently modified residue (Cys592) by beta-mercaptoethanol at its active site. By correlating the structural information of JBU with the available biophysical and biochemical data on insecticidal properties of plant ureases, it is hypothesized that the amphipathic beta-hairpin located in the entomotoxic peptide region of plant ureases might form a membrane insertion beta-barrel as found in beta-poreforming toxins
-
crystal structure determined at 2.2 A
-
urease is inactive in the crystalline form, possibly due to conformational restrictions associated with a lid covering the active site
-
wild-type and mutant enzyme
-
crystal structure determined at 2.0 A
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Methanol
-
100% activity
phenol
-
0.01% activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, 70 days, soluble enzyme loses 90% of its activity, alkylamine-immobilized enzyme loses 15% of the initial activity, the arylamine-immobilized enzyme loses 17% of its activity
-
20C, 50 mM phosphate buffer, pH 7.5, 50% glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, about 30% loss of activity after 2 months
-
4C, sodium phosphate buffer, pH 7.2, 1% bovine serum albumin, stable for 20 days
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
alphaH134A
-
no detectable activity, 53% of the nickel content of wild-type enzyme
alphaH136A
-
no detectable activity, 6% of the nickel content of wild-type enzyme
alphaH219A
-
1.9% of the activity of the wild-type enzyme, 80% of the nickel content of the wild type enzyme, very high Km-value compared to wild-type enzyme
alphaH246A
-
no detectable activity, 21% of the nickel content of wild-type enzyme
alphaH320A
-
normal nickel content, 0.003% of the activity of the wild-type enzyme, unlike wild-type enzyme no inactivation by diethyldicarbonate
alphaH321A
-
activity and nickel content are similar to wild-type enzyme
betaH39A
-
activity and nickel content are similar to wild-type enzyme
betaH41A
-
activity and nickel content are similar to wild-type enzyme
C319A
-
48% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value; no activity
C319D
-
0.03% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value
C319S
-
4.5% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value
D221A
-
low activity, small increase in Km-value and pH 5 optimum
G11P
P18314 and P18315 and P18316
UreB, subunit beta, analysis of urease activation
G18P
P18314 and P18315 and P18316
UreB, subunit beta, analysis of urease activation
gammaH96A
-
activity and nickel content are similar to wild-type enzyme
H219A
-
1000fold increased Km-value over that of the native enzyme
H219H
-
100fold increased Km-value over that of the native enzyme
H219Q
-
100fold increased Km-value over that of the native enzyme
H320A
-
100000fold deficiencies in rates, modest Km changes, disorders in the peptide flap covering their active sites, the pH-optimum is anomalous with optima near pH 6 and shoulders that extend to pH 9
H320N
-
100000fold deficiencies in rates, modest Km changes, disorders in the peptide flap covering their active sites, the pH-optimum is anomalous with optima near pH 6 and shoulders that extend to pH 9
H320Q
-
100000fold deficiencies in rates, modest Km changes, disorders in the peptide flap covering their active sites, the pH-optimum is anomalous with optima near pH 6 and shoulders that extend to pH 9
R336Q
-
0.0001fold decreased catalytic rate with near-normal pH dependence, unaffected Km-value, phenylglyoxal inactivates at over half the rate observed for the native enzyme
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
urea is estimated in different blood samples with the help of agar-immobilized urease and the results are consistent with those from clinical pathology laboratory through an autoanalyzer
biotechnology
-
immobilization of urease on arylamine glass beads results in improved thermal, storage and operational stability. Because of inertness of support and stability of immobilized urease, the preparation can find applications in artificial kidney and urea estimation in biological fluids
medicine
-
use of jack bean urease as a model enzyme for Helicobacter pylori urease. Development of organobismuth components without antifungal activity against Saccharomyces cerevisiae but with antibacterial activity
food industry
-
the enzyme is applicable to elimination of urea in Chinese rice wine
food industry
Enterobacter sp. R-SYB082
-
the enzyme is applicable to elimination of urea in Chinese rice wine
-
medicine
-
lack of enzyme activity in most enterohaemorrhagic strains is due to a premature stop codon in ureD encoding a chaperone protein of the urease gene cluster
biotechnology
-
soybean (Glycine max) urease is immobilized on alginate and chitosan beads and shows improved stability. This could have a potential role in haemodialysis machines
medicine
-
enhancement of intragastric enzyme activity is not strictly a function of pH-value, but is related to differential effects of the availability of nickel, which is required for enzyme activity
medicine
-
the action of urease in the upper intestinal tract is exploited in a non-invasive urease breath test for the diagnosis of bacterial infections of Helicobacter pylori: 13C- or 14C-labelled urea is ingested, and if the bacterium is present in the stomach, the urea is converted into isotope-labelled carbon dioxide. This is absorbed into the blood and exhaled in the breath, where it is detected by mass spectrometer or scintillation counter
nutrition
-
elimination of the urea in alcoholic beverages
nutrition
Lactobacillus animalis MU-4
-
elimination of the urea in alcoholic beverages
-
biotechnology
-
in food industry immobilisation of acid urease on an inert carrier has the potential advantages of significant cost savings, improved stability or resistance to shear or inhibitory compound inactivation. Purified acid urease preparation is covalently immobilised onto biocompatible porous chitosan beads of different size. The kinetics of urea degradation in a model wine solution using this biocatalyst is of the pseudofirst order with respect to the urea concentration in the liquid bulk, the apparent pseudo-first order kinetic rate constant ranging from about two thirds to one fifth of that pertaining to free acid urease
nutrition
-
elimination of the urea in alcoholic beverages
synthesis
-
production of ammonia
nutrition
Lactobacillus fermentum IFO 14511
-
elimination of the urea in alcoholic beverages
-
nutrition
-
elimination of the urea in alcoholic beverages
nutrition
Lactobacillus reuteri RT-5
-
elimination of the urea in alcoholic beverages
-
nutrition
-
elimination of the urea in alcoholic beverages
nutrition
Lactobacillus ruminis PG-98
-
elimination of the urea in alcoholic beverages
-
nutrition
Streptococcus mitior
-
elimination of the urea in alcoholic beverages
nutrition
Streptococcus mitior PG-118
-
elimination of the urea in alcoholic beverages
-
nutrition
-
elimination of the urea in alcoholic beverages
nutrition
Streptococcus salivarius PG-202
-
elimination of the urea in alcoholic beverages
-