Application | Comment | Organism |
---|---|---|
food industry | the enzyme is applicable to elimination of urea in Chinese rice wine | Enterobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0195 | - |
Urea | - |
Enterobacter sp. | |
0.0195 | - |
Urea | 35°C, pH 4.5 | Enterobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
6 * 72000, SDS-PAGE | Enterobacter sp. |
430000 | - |
gel filtration | Enterobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. | - |
- |
- |
Enterobacter sp. | - |
R-SYB082 | - |
Enterobacter sp. R-SYB082 | - |
- |
- |
Enterobacter sp. R-SYB082 | - |
R-SYB082 | - |
Purification (Comment) | Organism |
---|---|
- |
Enterobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
101.7 | - |
- |
Enterobacter sp. |
109 | - |
- |
Enterobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Enterobacter sp. | CO2 + NH3 | - |
? | |
urea + H2O | - |
Enterobacter sp. R-SYB082 | CO2 + NH3 | - |
? | |
urea + H2O | - |
Enterobacter sp. | CO2 + 2 NH3 | - |
? | |
urea + H2O | - |
Enterobacter sp. R-SYB082 | CO2 + 2 NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 72000, SDS-PAGE | Enterobacter sp. |
homohexamer | 6 * 72000, SDS-PAGE | Enterobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
urease | - |
Enterobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Enterobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | 25°C: about 80% of maximal activity, 75°C: about 45% of maximal activity | Enterobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, stable | Enterobacter sp. |
50 | - |
very stable below 50°C | Enterobacter sp. |
55 | - |
30 min, 15% loss of activity | Enterobacter sp. |
60 | - |
30 min, about 25% loss of activity | Enterobacter sp. |
70 | - |
30 min, about 55% loss of activity | Enterobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Enterobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | pH 3.0: about 65% of maximal activity, pH 7.0: anout 75% of maximal activity | Enterobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | - |
Enterobacter sp. |
4 | 6 | 35°C, 30 min, stable | Enterobacter sp. |