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Literature summary for 3.5.1.5 extracted from
- High-level expression and characterization of recombinant acid urease for enzymatic degradation of urea in rice wine (2014), Appl. Microbiol. Biotechnol., 99, 301-308.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | use of recombinant acid urease for enzymatic degradation of urea in rice wine. Ethylcarbamate, a carcinogenic compound, is formed from urea and ethanol in rice wine, therefore enzymatic elimination of urea is always attractive | Limosilactobacillus reuteri |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ureA, encoded in gene cluster ureABCEFGD, cloning in Escherichia coli strain DH5alpha, recombinnat expression in Lactobacillus lactis NZ9000, Lactobacillus lactis NZ3900, and Lactobacillus plantarum CICC6002. Optimization of cultivation process for enhancing production of the recombinant acid urease, overview | Limosilactobacillus reuteri |
| gene ureB, encoded in gene cluster ureABCEFGD, cloning in Escherichia coli strain DH5alpha, recombinnat expression in Lactobacillus lactis NZ9000, Lactobacillus lactis NZ3900, and Lactobacillus plantarum CICC6002. Optimization of cultivation process for enhancing production of the recombinant acid urease, overview | Limosilactobacillus reuteri |
| gene ureC, encoded in gene cluster ureABCEFGD, cloning in Escherichia coli strain DH5alpha, recombinnat expression in Lactobacillus lactis NZ9000, Lactobacillus lactis NZ3900, and Lactobacillus plantarum CICC6002. Optimization of cultivation process for enhancing production of the recombinant acid urease, overview | Limosilactobacillus reuteri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11100 | - |
1 * 67000, alpha-subunit, + 1 * 14200, beta-subunit, + 1 * 11100, gamma-subunit, SDS-PAGE | Limosilactobacillus reuteri |
| 14200 | - |
1 * 67000, alpha-subunit, + 1 * 14200, beta-subunit, + 1 * 11100, gamma-subunit, SDS-PAGE | Limosilactobacillus reuteri |
| 67000 | - |
1 * 67000, alpha-subunit, + 1 * 14200, beta-subunit, + 1 * 11100, gamma-subunit, SDS-PAGE | Limosilactobacillus reuteri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Limosilactobacillus reuteri | by incubating the enzyme (50 U/L) at 20°C for 60 h, about 95.8% of urea in rice wine is removed | ? | - |
? |  |
| additional information | Limosilactobacillus reuteri CICC6124 | by incubating the enzyme (50 U/L) at 20°C for 60 h, about 95.8% of urea in rice wine is removed | ? | - |
? | |
| urea + H2O | Limosilactobacillus reuteri | - |
CO2 + 2 NH3 | - |
? | |
| urea + H2O | Limosilactobacillus reuteri CICC6124 | - |
CO2 + 2 NH3 | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | purified recombinant enzyme, about 90% residual activity remains in the presence of 15% ethanol | Limosilactobacillus reuteri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limosilactobacillus reuteri | B3XPZ2 | subunit alpha; the sequence of urease gene cluster ureABCEFGD in Lactobacillus reuteri CICC6124 is identical to that of the strain Lactobacillus reuteri 100-23 | - |
| Limosilactobacillus reuteri | B3XPZ3 | subunit beta; the sequence of urease gene cluster ureABCEFGD in Lactobacillus reuteri CICC6124 is identical to that of the strain Lactobacillus reuteri 100-23 | - |
| Limosilactobacillus reuteri | B3XPZ4 | subunit gamma; the sequence of urease gene cluster ureABCEFGD in Lactobacillus reuteri CICC6124 is identical to that of the strain Lactobacillus reuteri 100-23 | - |
| Limosilactobacillus reuteri CICC6124 | B3XPZ2 | subunit alpha; the sequence of urease gene cluster ureABCEFGD in Lactobacillus reuteri CICC6124 is identical to that of the strain Lactobacillus reuteri 100-23 | - |
| Limosilactobacillus reuteri CICC6124 | B3XPZ3 | subunit beta; the sequence of urease gene cluster ureABCEFGD in Lactobacillus reuteri CICC6124 is identical to that of the strain Lactobacillus reuteri 100-23 | - |
| Limosilactobacillus reuteri CICC6124 | B3XPZ4 | subunit gamma; the sequence of urease gene cluster ureABCEFGD in Lactobacillus reuteri CICC6124 is identical to that of the strain Lactobacillus reuteri 100-23 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Lactobacilli by anion exchange and hydrophobic interaction chromatography, ultrafiltration and another, different step of anion exchange chromatography | Limosilactobacillus reuteri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 354 | - |
purified recombinnat enzyme, pH 3.0, 70°C | Limosilactobacillus reuteri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | by incubating the enzyme (50 U/L) at 20°C for 60 h, about 95.8% of urea in rice wine is removed | Limosilactobacillus reuteri | ? | - |
? | |
| additional information | by incubating the enzyme (50 U/L) at 20°C for 60 h, about 95.8% of urea in rice wine is removed | Limosilactobacillus reuteri CICC6124 | ? | - |
? | |
| urea + H2O | - |
Limosilactobacillus reuteri | CO2 + 2 NH3 | - |
? | |
| urea + H2O | - |
Limosilactobacillus reuteri CICC6124 | CO2 + 2 NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 67000, alpha-subunit, + 1 * 14200, beta-subunit, + 1 * 11100, gamma-subunit, SDS-PAGE | Limosilactobacillus reuteri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid urease | - |
Limosilactobacillus reuteri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Limosilactobacillus reuteri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
the purified recombinant enzyme is stable at pH 3.0-6.0 at temperatures below 50°C | Limosilactobacillus reuteri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Limosilactobacillus reuteri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | the purified recombinant enzyme is stable at pH 3.0-6.0 at temperatures below 50°C | Limosilactobacillus reuteri |
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