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Literature summary for 3.5.1.5 extracted from
- Purification and properties of urease from bovine rumen (1977), Biochem. J., 163, 495-501.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | slight stimulation | Bos taurus |  |
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol, 50 mM, stabilizes | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Acetohydroxamate | reversible | Bos taurus | |
| Ba2+ | - |
Bos taurus | |
| Ca2+ | - |
Bos taurus | |
| Cd2+ | - |
Bos taurus | |
| Cu2+ | - |
Bos taurus | |
| Hg2+ | - |
Bos taurus | |
| Hydroxyurea | reversible | Bos taurus | |
| Mg2+ | - |
Bos taurus | |
| Mn2+ | - |
Bos taurus | |
| NEM | 0.1 mM, complete inhibition | Bos taurus | |
| Ni2+ | - |
Bos taurus | |
| p-chloromercuribenzene sulfonate | 0.1 mM, complete inhibition | Bos taurus | |
| Sr2+ | - |
Bos taurus | |
| Zn2+ | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
Urea | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
gel filtration | Bos taurus |
| 130000 | - |
sucrose density gradient centrifugation | Bos taurus |
| 135000 | - |
non-denaturing PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| rumen | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 59.9 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Bos taurus | CO2 + NH3 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | pH 7.0: 52% of maximal activity, pH 8.5: 42% of maximal activity | Bos taurus |
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Release 2023.1 (January 2023)
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