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Literature summary for 3.5.1.5 extracted from
- Urease from Staphylococcus saprophyticus: purification, characterization and comparison to Staphylococcus xylosus urease (1994), Arch. Microbiol., 161, 393-399.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Acetohydroxamate | - |
Staphylococcus saprophyticus |  |
| Phenylurea | weak non-competitive | Staphylococcus saprophyticus | |
| Semicarbazide | competitive | Staphylococcus saprophyticus | |
| Urea | competitive | Staphylococcus saprophyticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.5 | - |
Urea | - |
Staphylococcus saprophyticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Nickel | enzyme contains 4.09 atoms of nickel per molecule | Staphylococcus saprophyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13900 | - |
alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE | Staphylococcus saprophyticus |
| 20400 | - |
alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE | Staphylococcus saprophyticus |
| 72400 | - |
alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE | Staphylococcus saprophyticus |
| 410000 | - |
non-denaturing PAGE | Staphylococcus xylosus |
| 420000 | - |
non-denaturing PAGE | Staphylococcus saprophyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus saprophyticus | - |
- |
- |
| Staphylococcus xylosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Staphylococcus saprophyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1979 | - |
- |
Staphylococcus saprophyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Staphylococcus saprophyticus | CO2 + NH3 | - |
? | |
| urea + H2O | - |
Staphylococcus xylosus | CO2 + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE | Staphylococcus saprophyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Staphylococcus saprophyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
in citrate buffer | Staphylococcus saprophyticus |
| 7 | - |
in citrate buffer | Staphylococcus saprophyticus |
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