Application | Comment | Organism |
---|---|---|
medicine | a novel immunoconjugate (L-DOS47) is developed and characterized as a therapeutic agent for tumors expressing CEACAM6. The single domain antibody AFAIKL2, which targets CEACAM6, is expressed in the Escherichia coli BL21 (DE3) pT7-7 system. High purity urease is extracted and purified from Jack bean meal. AFAIKL2 is activated using N-succinimidyl [4-iodoacetyl]aminobenzoate (SIAB) as the cross-linker and then conjugated to urease. The specificity and cytotoxicity of L-DOS47 is confirmed by screening in four cell lines (BxPC-3, A549, MCF7, and CEACAM6-transfected H23). BxPC-3, a CEACAM6-expressing cell line is most susceptible to L-DOS47. L-DOS47 is a potential therapeutic agent in human phase I clinical studies for nonsmall cell lung cancer | Canavalia ensiformis |
Cloned (Comment) | Organism |
---|---|
expression in the Escherichia coli BL21 (DE3) pT7-7 system | Canavalia ensiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canavalia ensiformis | P07374 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Canavalia ensiformis |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 91000 | Canavalia ensiformis |