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Literature summary for 3.5.1.5 extracted from

  • Park, I.S.; Hausinger, R.P.
    Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding and catalysis (1993), Protein Sci., 2, 1034-1041.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.5

Protein Variants

Protein Variants Comment Organism
alphaH134A no detectable activity, 53% of the nickel content of wild-type enzyme Klebsiella aerogenes
alphaH136A no detectable activity, 6% of the nickel content of wild-type enzyme Klebsiella aerogenes
alphaH219A 1.9% of the activity of the wild-type enzyme, 80% of the nickel content of the wild type enzyme, very high Km-value compared to wild-type enzyme Klebsiella aerogenes
alphaH246A no detectable activity, 21% of the nickel content of wild-type enzyme Klebsiella aerogenes
alphaH320A normal nickel content, 0.003% of the activity of the wild-type enzyme, unlike wild-type enzyme no inactivation by diethyldicarbonate Klebsiella aerogenes
alphaH321A activity and nickel content are similar to wild-type enzyme Klebsiella aerogenes
betaH39A activity and nickel content are similar to wild-type enzyme Klebsiella aerogenes
betaH41A activity and nickel content are similar to wild-type enzyme Klebsiella aerogenes
gammaH96A activity and nickel content are similar to wild-type enzyme Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 Urea mutant betaH41A Klebsiella aerogenes
1.5
-
 Urea mutant betaH39A Klebsiella aerogenes
1.6
-
 Urea mutant alphah312A Klebsiella aerogenes
1.9
-
 Urea mutant gammaH96A Klebsiella aerogenes
2
-
 Urea mutant alphaH321A Klebsiella aerogenes
2.3
-
 Urea wild-type enzyme Klebsiella aerogenes
8.3
-
 Urea mutant alphaH320A Klebsiella aerogenes
1100
-
 Urea mutant alphaH219A Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
 Klebsiella aerogenes CO2 + NH3
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 h, 30% loss of activity, wild-type enzyme Klebsiella aerogenes
60
-
 2 h, 55% loss of activity, wild-type enzyme. 2 h, complete inactivation of mutant enzymes betaH39A and alphaH320A Klebsiella aerogenes
70
-
 1 h, 87% loss of activity, wild-type enzyme, 58% loss of activity of mutant enzyme alphaG312A, complete inactivation of mutant enzymes gammaH96A, betaH39A, betaH41A, alphaH219A, alphaH320A and alphaH321A Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 mutant enzyme alphaH320A Klebsiella aerogenes
7.6
-
 wild-type enzyme Klebsiella aerogenes