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3.4.24.80: membrane-type matrix metalloproteinase-1

This is an abbreviated version!
For detailed information about membrane-type matrix metalloproteinase-1, go to the full flat file.

Word Map on EC 3.4.24.80

Reaction

endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at Asn37-/-Leu. Other bonds hydrolysed include Gly35-/-Ile in the propeptide of collagenase 3, and Asn341-/-Phe, Asp441-/-Leu and Gln354-/-Thr in the aggrecan interglobular domain =

Synonyms

ectMMP-14, gelatinase A, interstitial MT1-MMP, matrix metalloprotease 14, matrix metalloproteinase 14, matrix metalloproteinase MT 1, matrix metalloproteinase MT-MMP-1, matrix metalloproteinase MT1-MMP, matrix metalloproteinase-14, membrane type 1 matrix, membrane type 1 matrix metalloproteinase, membrane type 1 MMP, membrane type 1-matrix metalloproteinase, membrane type 1-MMP, membrane type I MMP, membrane type matrix metalloproteinase, membrane type MMP-1, membrane type MT1-MMP, membrane type-1 matrix metalloprotease, membrane type-1 matrix metalloprotease 1, membrane type-1 matrix metalloproteinase, membrane type-1 MMP, membrane type-I matrix metalloproteinase, membrane type-I MMP, membrane-anchored matrix metalloproteinase 14, membrane-type 1 matrix metalloproteinase, membrane-type 1 matrix metalloproteinase 1, membrane-type 1 MMP, membrane-type 1-matrix metalloproteinase, membrane-type I matrix metalloproteinase, membrane-type matrix metalloproteinase MT1-MMP, membrane-type matrix metalloproteinase-1, membrane-type metalloproteinase MT1-MMP, membrane-type MMP, metalloproteinase, MMP-14, MMP-2, MMP-8, MMP14, mmp14a, mmp14b, More, MT-MMP-1, MT-MMP1, MT1-MMP, MT1-MMP/MMP-14, MT1-MPP, MTI-MMP, pericellular collagenase, proteinase, matrix metallo-, sMT1-MMP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.80 membrane-type matrix metalloproteinase-1

Cloned

Cloned on EC 3.4.24.80 - membrane-type matrix metalloproteinase-1

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
18 kDa inactive fragment ranging from Tyr 112 to Ala 255, 21 kDa fragment ranging from Tyr 122 to Gly 284 and truncated form lacking the cytosolic domain
C-terminal truncated form containing the hemopexin-like domain
catalytic and hinge domain
catalytic and hinge domains of MMP-14 (Tyr112-Ile318), containing an N-terminal methionine and C-terminal hexa-histidines (chMMP-14) expressed as inclusion body in Escherichia coli
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catalytic domain
catalytic domain of MT1-MMP expressed in Escherichia coli, MT1-MMP cDNAs cloned into vector pcDNA3-zeo
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cloned into a pcDNA3.0 expression vector
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cloned into pSG5 vector, expression in COS-1 cells
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cloned intop pCR-Blunt II TOPO vector
expressed in adenovirus
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expressed in breast adenocarcinoma cells
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expressed in COS-7 cells
expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells and COS-7 cells
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expressed in lentivirus
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expressed in LNCaP cells
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expressed in mouse bone marrow-derived stromal cells
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expressed in polarized human cancer cell lines A375, 451Lu, and HT-1080
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expressed in prostate cancer LNCaP cells
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expression analysis
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expression in non-malignant monkey kidney epithelial BS-C-1 and CV-1 cells by co-infecting the cells with the vTF7-3 and vTF-MT1 vaccinia viruses
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gene MMP14, quantitative RT-PCR enzyme expression analysis
gene MMP14, recombinant coexpression of MMP14 with CCN5 in MCF-7 and CHO-K1 cells
gene MMP14, recombinant expression in Escherichia coli strain BL21(DE3)
gene MMP14, recombinant expression of C-terminally His-tagged wild-type and mutant Co2+-MT1-MMPs and apoenzymes in Escherichia coli strain BL21(DE3), the plasmid contains the catalytic domain of human MT1-MMP together with the hinge linker to the hemopexin-like domain (residues 112-292). Interaction of the His-tag with the protein cleft, modelling, overview
gene MMP14, recombinant expression of N-terminally HA3-tagged and C-terminally EGFP-tagged wild-type and mutant R111H enzymes in in MRC-5V1 immortalized human fetal lung fibroblasts, in which mutant protein localization is unlikely to be affected by dimerization with endogenous MMP14
gene MMP14, recombinant expression of wild-type and mutant enzymes in COS-1 cells
gene MMP14, recombinant expression of wild-type enzyme, a soluble enzyme form, and different enzyme MT1-MMP domain constructs in MCF-7 cells, overview
gene MMP14, transient recombinant overexpression of MT1-MMP in HT-1080 cells, quantitative expression analysis
gene mmp14a, recombinant expression of C-terminally HA-tagged enzyme in in MRC-5V1 immortalized human fetal lung fibroblasts, in which mutant protein localization is unlikely to be affected by dimerization with endogenous MMP14
gene mmp14b, recombinant expression of C-terminally HA-tagged enzyme in in MRC-5V1 immortalized human fetal lung fibroblasts, in which mutant protein localization is unlikely to be affected by dimerization with endogenous MMP14
MT1-MMP cDNA subcloned between the HindIII and EcoRI sites of pCDNA3.1 Zeo+ mammalian expression vector, annealed olignucleotides coding for huMT1-MMP signal sequence cloned upstream of ECFP in the NheI-AgeI sites of the pEGFP-C1 vector, amplified residues 283-582 of huMT1-MMP cloned into the BgIII-EcoRI sites of huMT1SSc1 in-frame with EGFP
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MT1-MMP expression in both the epithelial and stromal elements of head and neck squamous cell carcinoma cell line, low enzyme activity
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MT1-MMP, lacking the C-terminal transmembrane and cytoplasmic domains, expression in Pichia pastoris
recombinant cdMT1-MMP expressed in Escherichia coli, recombinant deltaTM-MT1-MMP expressed in Pichia pastoris
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recombinant expression of the truncation mutant MT1EADELTATM in Escherichia coli, expression of the PEX-domain-deleted MT1-MMP mutant
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recombinant expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3)pLysS in inclusion bodies
stable expression of FLAG-tagged MT1-MMP in A-431 cells, co-expression with Myc-tagged MT1-MMP
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the catalytic domain (Tyr112-Val335) is expressed in Escherichia coli BL21 cells
truncated forms, mutations and wild type
two truncated forms: CAT domain and CAT/PEX domain
wild-type and various mutants: deletion of catalytic domain, deletion of POX domain, deletion of cytoplasmic tail, deletion of 8, 8 or 12 amino acids at C-terminus, all deletions except catalytic and PEX domain reduced internalization of the protein