Application | Comment | Organism |
---|---|---|
medicine | full enzymatic activity of MT1-MMP is required for a specific up-regulation of VEGF-A through an activation of Src tyrosine kinase pathways | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
MT1-MMP cDNA subcloned between the HindIII and EcoRI sites of pCDNA3.1 Zeo+ mammalian expression vector, annealed olignucleotides coding for huMT1-MMP signal sequence cloned upstream of ECFP in the NheI-AgeI sites of the pEGFP-C1 vector, amplified residues 283-582 of huMT1-MMP cloned into the BgIII-EcoRI sites of huMT1SSc1 in-frame with EGFP | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C574S | substitution in the cytoplasmic domain, reduction of pro-MMP2 activation, no up-regulation of VEGF expression | Homo sapiens |
E240A | substitution in the active site, unable to activate exogenous pro-MMP2, fails to induce VEGF mRNA expression | Homo sapiens |
additional information | partial deletion of the catalytic domain deltacd or the cytoplasmic tail delta577 of MT1-MMP, unable to activate exogenous pro-MMP2, no up-regulation of VEGF expression | Homo sapiens |
S577A | substitution in the cytoplasmic domain, pro-MMP2-activating capacity not affected, similar VEGF upregulation | Homo sapiens |
T567A | substitution in the cytoplasmic domain, pro-MMP2-activating capacity not affected, similar VEGF upregulation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BB94 | reduces the VEGF mRNA levels in MT clones but does not affect the basal VEGF mRNA levels in control clones | Homo sapiens | |
genistein | markedly suppresses the VEGF mRNA induction in MT clones without affecting the VEGF expression in control clones | Homo sapiens | |
herbimycin-A | strongly inhibits up-regulation effect of VEGF-A by MT1-MMP | Homo sapiens | |
additional information | TIMP-1 does not block up-regulation of VEGF-A by MT1-MMP, aprotinin, Pefabloc SC, leupeptin and pepstatin A fail to modulate the VEGF expression, wortmannin, PD98059 nor SB203580 affect MT1-MMP-mediated VEGF induction | Homo sapiens | |
RO28-2653 | reduces the VEGF mRNA levels in MT clones but does not affect the basal VEGF mRNA levels in control clones | Homo sapiens | |
TIMP-2 | blocks up-regulation of VEGF-A by MT1-MMP | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MCF-7 cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
membrane type 1 matrix metalloproteinase | - |
Homo sapiens |
MT1-MMP | - |
Homo sapiens |