3.4.22.B70: SENP1 peptidase

This is an abbreviated version, for detailed information about SENP1 peptidase, go to the full flat file.

Reaction

The enzyme catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO-1, SUMO-2 and SUMO-3 to their mature forms and deconjugation of SUMO-1, SUMO-2 and SUMO-3 from targeted proteins. Deconjugates SUMO-1 from homeodomain-interacting protein kinase 2. Deconjugates SUMO-1 from histone deacetylase 1, which decreases its transcriptional repression activity. Cleavage of Gly97-/-His98 bond in the SUMO-1 precursor with release of the propeptide His-Ser-Thr-Val. Cleavage of Gly93-/-Val94 bond in the SUMO-2 precursor with release of the propeptide Val94-Thyr. Cleavage of the Gly92-/-Val93 in the SUMO-3 precursor with release of the propeptide Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe. =

Synonyms

C13orf22l, SENP, SENP1, sentrin-specific protease 1, sentrin/SUMO-specific protease 1, small ubiquitin-like modifier protein-specific protease 1, small ubiquitin-related modifier-specific isopeptidase, SUMO isopeptidase, SUMO protease, SUMO protein-specific protease 1, SUMO-specific isopeptidase, SUMO-specific protease, SUMO-specific protease 1, ubiquitin-specific protease-like 1, USPL1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B70 SENP1 peptidase

Engineering

Engineering on EC 3.4.22.B70 - SENP1 peptidase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C236S
site-directed mutagenesis, the mutant is not inhibited by suicide inhibitor Strep-TEV-HA-SUMO-vinylmethylester in contrast to the wild-type enzyme
C602A
-
completely inactive in processing of SUMO-2 and in deconjugating SUMO-2
C603A
-
the mutant is unable to desumoylate the (SUMO-1)-homeodomain-interacting protein kinase 2 conjugate
D441A
-
mutant protein is indistinguishable from that of the wild-type protein in both processing and deconjugation
D468A
-
mutated protein is only slightly impaired in processing and not at all in deconjugation
D550A
-
completely inactive in processing of SUMO-2 and in deconjugating SUMO-2
F496A
-
altered protein retains significant levels of deconjugation activity
H529A
-
altered protein retains significant levels of deconjugation activity
H533A
-
completely inactive in processing of SUMO-2 and in deconjugating SUMO-2
Q596A
-
mutant of SENP1 is severely impaired in both deconjugation and processing
R511A
-
mutant has reduced activity, but is unaffected in deconjugation
V532A
-
mutation has no effect on either deconjugation or processing
W229L
site-directed mutagenesis, mutation to the residue found in USP2 and other ubiquitin-specific USPs dramatically impairs SUMO binding and cleavage
W237F
site-directed mutagenesis, mutation to the residue found in USP2 and other ubiquitin-specific USPs dramatically impairs SUMO binding and cleavage
W512A
-
mutant has a reduced deconjugation activity in vitro, its deconjugation activity in vivo is only impaired to a small extent
C599S
-
site-directed mutagenesis, the active site mutant precipitates SUMO1ylated proteins but not SUMO2/3-modified substrate
additional information