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Literature summary for 3.4.22.B70 extracted from

  • Shen, L.N.; Dong, C.; Liu, H.; Naismith, J.H.; Hay, R.T.
    The structure of SENP1-SUMO-2 complex suggests a structural basis for discrimination between SUMO paralogues during processing (2006), Biochem. J., 397, 279-288.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the catalytic core domain of SENP1 (amino acids 415-643) and mutants are cloned into the vector pEHISTEV and expressed as a N-terminally His-tagged protein. The recombinant proteins are expressed in Escherichia coli Bl21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
SENP1 crystallization is performed at 20°C using a sitting drop vapour-diffusion method. Single diamond-shaped crystals are grown after 2 days from equal volumes of protein solution (20 mg/ml in 20 mM Tris/HCl, pH 8.0, and 50 mM NaCl) and reservoir solution containing 100 mM CoCl2, 0.1 M Mes, pH 6.5, and 1.8 M (NH4)2SO4. The structure of SENP1 is determined to 2.45 A. NaBH4 is used to trap a stable thiohemiacetal transition-state analogue between Cys602 of SENP1 and Gly92 of SUMO-2, determination of the structure of this complex to 3.2 A. Crystallization and structure determination of the SENP1-SUMO-2 complex Homo sapiens

Protein Variants

Protein Variants Comment Organism
C602A completely inactive in processing of SUMO-2 and in deconjugating SUMO-2 Homo sapiens
D441A mutant protein is indistinguishable from that of the wild-type protein in both processing and deconjugation Homo sapiens
D468A mutated protein is only slightly impaired in processing and not at all in deconjugation Homo sapiens
D550A completely inactive in processing of SUMO-2 and in deconjugating SUMO-2 Homo sapiens
F496A altered protein retains significant levels of deconjugation activity Homo sapiens
H529A altered protein retains significant levels of deconjugation activity Homo sapiens
H533A completely inactive in processing of SUMO-2 and in deconjugating SUMO-2 Homo sapiens
additional information residues 415643 of SENP1 are expressed and purified from Escherichia coli and are shown to be catalytically active in SUMO processing and deconjugation Homo sapiens
Q596A mutant of SENP1 is severely impaired in both deconjugation and processing Homo sapiens
R511A mutant has reduced activity, but is unaffected in deconjugation Homo sapiens
V532A mutation has no effect on either deconjugation or processing Homo sapiens
W512A mutant has a reduced deconjugation activity in vitro, its deconjugation activity in vivo is only impaired to a small extent Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9P0U3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(SUMO-1)-Ran GTPase-activating protein 1 conjugate + H2O rates at which SUMO-1, SUMO-2 and SUMO-3 are deconjugated from RanGAP1 are indistinguishable Homo sapiens SUMO-1 + Ran GTPase-activating protein 1
-
?
(SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O rates at which SUMO-1, SUMO-2 and SUMO-3 are deconjugated from RanGAP1 are indistinguishable Homo sapiens SUMO-2 + Ran GTPase-activating protein 1
-
?
(SUMO-2)n-glutathione S-transferase-promyelocytic leukemia protein conjugate + H2O glutathione S-transferase-promyelocytic leukemia protein bearing a polymeric chain of SUMO-2 Homo sapiens ?
-
?
(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O rates at which SUMO-1, SUMO-2 and SUMO-3 are deconjugated from RanGAP1 are indistinguishable Homo sapiens SUMO-3 + Ran GTPase-activating protein 1
-
?
SUMO-1 precursor + H2O pre-SUMO-1 is processed rapidly Homo sapiens SUMO-1 + His-Ser-Thr-Val
-
?
SUMO-2 precursor + H2O pre-SUMO-2 is processed slowly Homo sapiens SUMO-2 + Val-Tyr
-
?
SUMO-3 precursor + H2O pre-SUMO-3 is processed at an intermediate rate Homo sapiens SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
-
?

Synonyms

Synonyms Comment Organism
SENP1
-
Homo sapiens
sentrin-specific protease 1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens