3.4.22.B70: SENP1 peptidase

This is an abbreviated version, for detailed information about SENP1 peptidase, go to the full flat file.

Reaction

The enzyme catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO-1, SUMO-2 and SUMO-3 to their mature forms and deconjugation of SUMO-1, SUMO-2 and SUMO-3 from targeted proteins. Deconjugates SUMO-1 from homeodomain-interacting protein kinase 2. Deconjugates SUMO-1 from histone deacetylase 1, which decreases its transcriptional repression activity. Cleavage of Gly97-/-His98 bond in the SUMO-1 precursor with release of the propeptide His-Ser-Thr-Val. Cleavage of Gly93-/-Val94 bond in the SUMO-2 precursor with release of the propeptide Val94-Thyr. Cleavage of the Gly92-/-Val93 in the SUMO-3 precursor with release of the propeptide Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe. =

Synonyms

C13orf22l, SENP, SENP1, sentrin-specific protease 1, sentrin/SUMO-specific protease 1, small ubiquitin-like modifier protein-specific protease 1, small ubiquitin-related modifier-specific isopeptidase, SUMO isopeptidase, SUMO protease, SUMO protein-specific protease 1, SUMO-specific isopeptidase, SUMO-specific protease, SUMO-specific protease 1, ubiquitin-specific protease-like 1, USPL1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B70 SENP1 peptidase

Substrates Products

Substrates Products on EC 3.4.22.B70 - SENP1 peptidase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(SUMO-1)-androgen receptor conjugate + H2O
SUMO-1 + androgen receptor conjugate
show the reaction diagram
(SUMO-1)-histone deacetylase 1 conjugate + H2O
SUMO-1 + histone deacetylase 1
show the reaction diagram
(SUMO-1)-homeodomain-interacting protein kinase 2 conjugate + H2O
SUMO-1 + homeodomain-interacting protein kinase 2 conjugate
show the reaction diagram
(SUMO-1)-K2P1 potassium channel + H2O
SUMO-1 + K2P1 potassium channel
show the reaction diagram
-
silent K2P1 channels in excised plasma membrane patches are activated by SENP1
-
-
?
(SUMO-1)-K2P1 subunit of potassium channel + H2O
SUMO-1 + K2P1 subunit of potassium channel
show the reaction diagram
-
SUMO1 is conjugated to the epsilon-amino group of Lys274 of the K2P1 potassium channel. When mutated to Gln, Arg, Glu, Asp, Cys, or Ala, the channels are constitutively active and insensitive to SUMO1 and SENP1. Wild-type channels in plasma membrane have two K2P1 subunits and assemble with two SUMO1 monomers. Although channels engineered with one Lys274 site carry just one SUMO1 they are activated and silenced by SENP1 and SUMO1 like wild-type channels
-
-
?
(SUMO-1)-Ran GTPase-activating protein 1 conjugate + H2O
SUMO-1 + Ran GTPase-activating protein 1
show the reaction diagram
-
rates at which SUMO-1, SUMO-2 and SUMO-3 are deconjugated from RanGAP1 are indistinguishable
-
-
?
(SUMO-1)-RanGAP1 conjugate + H2O
SUMO-1 + RanGAP1
show the reaction diagram
-
SENP1 binding is accompanied by a conformational change in the substrate
-
-
?
(SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O
SUMO-2 + Ran GTPase-activating protein 1
show the reaction diagram
-
rates at which SUMO-1, SUMO-2 and SUMO-3 are deconjugated from RanGAP1 are indistinguishable
-
-
?
(SUMO-2)-RanGAP1 conjugate + H2O
SUMO-2 + RanGAP1
show the reaction diagram
-
SENP1 binding is accompanied by a conformational change in the substrate
-
-
?
(SUMO-2)n-glutathione S-transferase-promyelocytic leukemia protein conjugate + H2O
?
show the reaction diagram
-
glutathione S-transferase-promyelocytic leukemia protein bearing a polymeric chain of SUMO-2
-
-
?
(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O
SUMO-3 + Ran GTPase-activating protein 1
show the reaction diagram
-
rates at which SUMO-1, SUMO-2 and SUMO-3 are deconjugated from RanGAP1 are indistinguishable
-
-
?
CrSUMO148 precursor + H2O
CrSUMO148 + peptide
show the reaction diagram
-
CrSUMO148 is a SUMO homolog from Chlamydomonas reinhardtii with 148 amino acids. SENP1 solely cleaves CrSUMO148 at the peptide bond after the first diglycine motif, although there are four putative cleavage sites in the primary amino acid sequence
-
-
?
CrSUMO148-conjugated protein + H2O
CrSUMO148 + protein
show the reaction diagram
-
CrSUMO96 is a SUMO homolog from Chlamydomonas reinhardtii with 148 amino acids
-
-
?
CrSUMO96 precursor + H2O
CrSUMO96 + peptide
show the reaction diagram
-
CrSUMO96 is a SUMO homolog from Chlamydomonas reinhardtii with 96 amino acids. SENP1 shows more processing activity for CrSUMO97 than for CrSUMO96
-
-
?
CrSUMO96-conjugated protein + H2O
CrSUMO96 + protein
show the reaction diagram
-
CrSUMO96 is a SUMO homolog from Chlamydomonas reinhardtii with 96 amino acids
-
-
?
CrSUMO97 precursor + H2O
CrSUMO97 + peptide
show the reaction diagram
-
CrSUMO97 is a SUMO homolog from Chlamydomonas reinhardtii with 97 amino acids. SENP1 shows more processing activity for CrSUMO97 than for CrSUMO96
-
-
?
CrSUMO97-conjugated protein + H2O
CrSUMO97 + protein
show the reaction diagram
-
CrSUMO96 is a SUMO homolog from Chlamydomonas reinhardtii with 97 amino acids
-
-
?
Pin1 + H2O
?
show the reaction diagram
polyCrSUMO148 + H2O
?
show the reaction diagram
-
CrSUMO148 is a SUMO homolog from Chlamydomonas reinhardtii with 148 amino acids. polyCrSUMO148 chains aree completely deconjugated by SENP1
-
-
?
polyCrSUMO96 + H2O
?
show the reaction diagram
-
CrSUMO96 is a SUMO homolog from Chlamydomonas reinhardtii with 96 amino acids. polyCrSUMO96 chains aree completely deconjugated by SENP1. SENP1 displays a similar efficiency to deconjugate either polymeric CrSUMO96 or CrSUMO97 chains
-
-
?
polyCrSUMO97 + H2O
?
show the reaction diagram
-
CrSUMO97 is a SUMO homolog from Chlamydomonas reinhardtii with 97 amino acids. polyCrSUMO97 chains aree completely deconjugated by SENP1. SENP1 displays a similar efficiency to deconjugate either polymeric CrSUMO96 or CrSUMO97 chains
-
-
?
pro-small ubiquitin-related modifier + H2O
small ubiquitin-related modifier + ?
show the reaction diagram
-
SENP1 processes the precursor SUMO to its mature form by catalyzing the cleavage of a scissile peptide bond
-
-
?
Sharp-1 protein + H2O
?
show the reaction diagram
-
deSUMOylation by the enzyme
-
-
?
small ubiquitin-related modifier-protein + H2O
small ubiquitin-related modifier-protein + protein
show the reaction diagram
-
SUMO-specific proteases, SENPs, reversibly remove small ubiquitin-related modifier-protein, SUMO, from the SUMOylated proteins
-
-
r
SUMO-1 precursor + H2O
SUMO-1 + His-Ser-Thr-Val
show the reaction diagram
SUMO-2 precursor + H2O
SUMO-2 + Val-Tyr
show the reaction diagram
SUMO-3 precursor + H2O
SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
show the reaction diagram
SUMO-7-amido-4-methylcoumarin + H2O
SuMo + 7-amino-4-methylcoumarin
show the reaction diagram
SUMO-Elk-1 conjugate + H2O
SUMO + Elk-1
show the reaction diagram
SUMO-GATA 1 conjugate + H2O
SUMO + GATA1
show the reaction diagram
SUMO-homeodomain-interacting protein kinase 1 conjugate + H2O
SUMO + homeodomain-interacting protein kinase 1 conjugate
show the reaction diagram
-
-
-
-
?
SUMO-hypoxia-inducible factor 1 conjugate + H2O
SUMO + hypoxia-inducible factor 1
show the reaction diagram
SUMO-STAT3 protein conjugate + H2O
SUMO + STAT3 protein
show the reaction diagram
-
-
-
-
?
SUMO1ylated protein + H2O
SUMO1 + protein
show the reaction diagram
SUMO2ylated protein + H2O
SUMO2 + protein
show the reaction diagram
-
-
-
-
?
SUMO3ylated protein + H2O
SUMO3 + protein
show the reaction diagram
-
-
-
-
?
SUMOylated androgen receptor + H2O
androgen receptor + SUMO1
show the reaction diagram
-
deconjugation
-
-
?
SUMOylated peroxisome proliferator-activated receptor coactivator 1alpha + H2O
deSUMOylated peroxisome proliferator-activated receptor coactivator 1alpha + SUMO1
show the reaction diagram
-
deSUMOylation
-
-
?
additional information
?
-