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Literature summary for 3.4.22.B70 extracted from

  • Sharma, P.; Yamada, S.; Lualdi, M.; Dasso, M.; Kuehn, M.R.
    Senp1 is essential for desumoylating Sumo1-modified proteins but dispensable for Sumo2 and Sumo3 deconjugation in the mouse embryo (2013), Cell Rep., 3, 1640-1650.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B70

Cloned(Commentary)

Cloned (Comment) Organism
SENP1, recombinant expression of wild-type and mutant enzymes Mus musculus

Protein Variants

Protein Variants Comment Organism
C599S site-directed mutagenesis, the active site mutant precipitates SUMO1ylated proteins but not SUMO2/3-modified substrate Mus musculus
additional information construction of several allelic defective enzyme mutants and a truncated mutant Senp1, overview, and a Senp1-beta-geo fusion protein, Expression of the amino half of Senp1 reduces Senp2 levels Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide inhibits desumoylation Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SUMO1ylated protein + H2O Mus musculus main substrate SUMO1 + protein
-
 ?
SUMO2ylated protein + H2O Mus musculus
-
 SUMO2 + protein
-
 ?
SUMO3ylated protein + H2O Mus musculus
-
 SUMO3 + protein
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 129S6 genetic background or mixed 129S6/C57Bl6 mice
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant enzyme Senp1 can deconjugate terminal Sumo1 moieties on poly-Sumo2/3 chains Mus musculus ?
-
 ?
SUMO1ylated protein + H2O
-
 Mus musculus SUMO1 + protein
-
 ?
SUMO1ylated protein + H2O main substrate Mus musculus SUMO1 + protein
-
 ?
SUMO2ylated protein + H2O
-
 Mus musculus SUMO2 + protein
-
 ?
SUMO3ylated protein + H2O
-
 Mus musculus SUMO3 + protein
-
 ?

Synonyms

Synonyms Comment Organism
SENP1
-
 Mus musculus

General Information

General Information Comment Organism
malfunction embryos homozygous for this mutant allele, Senp1M1Mku show phenotypic abnormalities beginning at embryonic day (E) 11.5 with the majority dying between E12.5 and E14.5. Enzyme defects lead to accumulation of Sumo1-modified proteins in homozygous embryos, a lethal process, phenotypes, overview. Senp1Gt mutants express a truncated Senp1 affecting Sumo2/3 levels Mus musculus
physiological function enzyme Senp1 is essential for desumoylating Sumo1-modified proteins but dispensable for Sumo2 and Sumo3 deconjugation in the mouse embryo, overview. Senp1 is the primary activity for desumoylation of Sumo1-modified proteins Mus musculus