3.4.22.53: calpain-2
This is an abbreviated version!
For detailed information about calpain-2, go to the full flat file.
Word Map on EC 3.4.22.53
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3.4.22.53
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calpains
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calpastatin
-
mu-calpain
-
calcium-dependent
-
ca2+-dependent
-
proteinase
-
myofibrillar
-
cytoskeletal
-
cataract
-
zymography
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longissimus
-
meat
-
autolysis
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calpeptin
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tender
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casein
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cathepsins
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3.4.22.17
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postmortem
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calpain-mediated
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caspase-12
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lumborum
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autolyzed
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lens-specific
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talin
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alpha-spectrin
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canps
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cataractogenesis
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warner-bratzler
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spectrin
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slaughter
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semimembranosus
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calpain-specific
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desmin
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calpain-calpastatin
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lenses
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calpain-dependent
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atrogin-1
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medicine
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calpain-induced
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fodrin
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myofibril
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ca2+-activated
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calmodulin-like
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caseinolytic
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thoracis
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ca2+-requiring
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troponin-t
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micro-calpain
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calpain-like
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proteolyzed
- 3.4.22.53
- calpains
- calpastatin
- mu-calpain
-
calcium-dependent
-
ca2+-dependent
- proteinase
- myofibrillar
- cytoskeletal
- cataract
-
zymography
- longissimus
-
meat
-
autolysis
- calpeptin
-
tender
- casein
- cathepsins
-
3.4.22.17
-
postmortem
-
calpain-mediated
- caspase-12
- lumborum
-
autolyzed
-
lens-specific
- talin
- alpha-spectrin
-
canps
-
cataractogenesis
-
warner-bratzler
- spectrin
-
slaughter
- semimembranosus
-
calpain-specific
- desmin
-
calpain-calpastatin
- lenses
-
calpain-dependent
-
atrogin-1
- medicine
-
calpain-induced
- fodrin
- myofibril
-
ca2+-activated
-
calmodulin-like
-
caseinolytic
- thoracis
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ca2+-requiring
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troponin-t
- micro-calpain
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calpain-like
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proteolyzed
Reaction
broad endopeptidase specificity =
Synonyms
Cal II, calcium-activated neutral protease II, calpain 2, calpain II, calpain xCL-2 (Xenopus leavis), calpain-2, calpain-2-like, calpain2, CAPN II, CAPN2, CAPN2 g.p. (Homo sapiens), CPN2, cysteine protease, EC 3.4.22.17, EC 3.4.24.5, human calpain 2, m-calpain, milli-calpain, mitochondrial m-calpain, nCL-2, rat calpain 2
ECTree
3.4.22.53 calpain-2Advanced search results
results (
results (Substrates Products
Substrates Products on EC 3.4.22.53 - calpain-2
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-tubulin + H2O
?
-
complete digestion of alpha-tubulin with little effect on beta-tubulin
-
-
?
beta-lipotropin(61-91) + H2O
?
-
cleavage of the bonds: Thr76-Leu77, Lys84-Asn85 and Lys88-Lys89
-
-
?
Boc-Leu-Met-7-amido-4-chloromethylcoumarin + H2O
Boc-Leu-Met + 7-amino-4-chloromethylcoumarin
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10 microM, 20 min, 37 °C, with or without magnetic bead stimulation
-
-
?
collapsin response mediator protein-1 + H2O
?
-
collapsin response mediator protein-1 is cleaved by calpain-2 at the C-terminus
-
-
?
collapsin response mediator protein-2 + H2O
?
-
collapsin response mediator protein-2 is cleaved by calpain-2 at the C-terminus
-
-
?
collapsin response mediator protein-4 + H2O
?
-
collapsin response mediator protein-4 is cleaved by calpain-2 at the C-terminus
-
-
?
dihydropteridine reductase + H2O
?
-
the dihydropteridine reductase 29000 Da subunit is cleaved just before the 35th Ser and the 48th Val residue from the N-terminus, generating two new fragments of 21000 Da and 19000 Da which are more active than the native enzyme
-
-
?
focal adhesion kinase + H2O
?
-
the preferred calpain cleavage site is between the two C-terminal proline-rich regions after Ser-745
-
-
?
FRET-based substrate PLFAER + H2O
?
10 microM, pH 7.4, 1 mM of CaCl2 added to initiate the reaction
-
-
?
IP3R1 + H2O
?
-
in presence of Ca2þ, m-calpain cleaves IP3R1 in the endoplasmic lumen
-
-
?
mammalian actin-binding protein-1 + H2O
?
-
the preferred cleavage site occurs between the actin-binding domain and the proline-rich region, generating a C-terminal mAbp1 fragment
-
-
?
N-acetyl-LLY-7-amido-4-trifluoromethylcoumarin + H2O
N-acetyl-LLY + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
Na+/Ca2+ exchanger-1 + H2O
82 kDa fragment + ?
-
calcium-dependent proteolytic cleavage of Na+/Ca2+ exchanger-1 occurs in the caveolae vesicles
-
-
?
neurotensin + H2O
?
-
cleavage of the 3Tyr-4Glu bond and the 5Asn-6Lys bond
-
-
?
proteolysis-resistant fragment 130 + H2O
proteolysis-resistant fragment 45 + ?
-
-
-
-
?
proteolysis-resistant fragment 72 + H2O
proteolysis-resistant fragment 45 + ?
-
-
-
-
?
selenoprotein K + H2O
?
-
cleavage occurs only in unactivated macrophages, m-calpain cleavage at Arg81-Gly82 generates the two selenoprotein K isoforms
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
succinyl-Leu-Met-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Met + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
-
-
-
?
tert-butoxycarbonyl-Leu-Met-7-amido-4-chloromethylcoumarin + H2O
tert-butoxycarbonyl-Leu-Met + 7-amino-4-chloromethylcoumarin
-
-
-
-
?
tert-butyloxycarbonyl-L-leucyl-L-methionine-7-amido-4-chloromethylcoumarin + H2O
tert-butyloxycarbonyl-L-leucyl-L-methionine + 7-amino-4-chloromethylcoumarin
-
-
-
-
?
voltage-dependent anion channel + H2O
?
-
mitochondrial m-calpain truncates voltage-dependent anion channel in Ca2+-dependent manner
-
-
?
calmodulin-dependent protein kinase II deltaB + H2O
?
angiotensin II enhances the interaction between activated calpain-2 and Ca2+/calmodulin-dependent protein kinase II deltaB (CaMKIIdB), and promotes the degradation of CaMKIIdB by calpain-2 in the nuclei of hypertrophied cardiomyocytes. The depressed CaMKIIdeltaB downregulates the expression of antiapoptotic Bcl-2 leading to mitochondrial depolarization and release of cytochrome c which leads to apoptosis of hypertrophied cardiomyocytes
-
-
?
crystallin + H2O
?
-
alphaA crystallin in lenses from wild-type mice is proteolyzed by both calpain 2 and Lp82. Crystallins proteolyzed by calpain Lp82 are more susceptible to insolubilization than crystallins proteolyzed by calpain 2
-
-
?
GAP-43 + H2O
GAP-43-3 + ?
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GAP-43 cleavage at Ser41 residue in synaptosomes is mediated by m-calpain
-
-
?
GAP-43 + H2O
GAP-43-3 + ?
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a GAP-43 fragment, lacking about 40 N-terminal residues (named GAP-43-3), is produced by m-calpain. The fragment prevents complete cleavage of intact GAP-43 by m-calpain as a negative feedback
-
-
?
GAP-43 + H2O
GAP-43-3 + ?
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a GAP-43 fragment, lacking about 40 N-terminal residues (named GAP-43-3), is produced by m-calpain. The fragment prevents complete cleavage of intact GAP-43 by m-calpain as a negative feedback
-
-
?
IkappaBalpha + H2O
?
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a parallel pathway that degrades IkappaBalpha and activates NF-kappaB activation independently of the ubiquitin-proteasome pathway
-
-
?
MARCKS protein + H2O
?
MARCKS protein i.e. myristoylated alanine-rich C kinase substrate is a substrate of calpain-2 in the presence of Ca2+. Calpain-2 proteolysis of MARCKS promotes its interaction with lipids and ENaC at the plasma membrane to allow for the phosphatidylinositol 4,5-bisphosphate (PIP2)-dependent regulation of epithelial sodium channel (ENaC) activity in the kidney
-
-
?
MARCKS protein + H2O
?
MARCKS protein i.e. myristoylated alanine-rich C kinase substrate is a substrate of calpain-2 in the presence of Ca2+
-
-
?
myocillin + H2O
?
-
calpain II is responsible for the intracellular processing of myocilin in the lumen of the endoplasmic reticulum. It is proposed that this cleavage might regulate extracellular interactions of myocilin, contributing to the control of intraocular pressure
-
-
?
striatal-enriched protein tyrosine phosphatase + H2O
?
calpain-2 activation cleaves striatal-enriched protein tyrosine phosphatase and activates STEP-mediated pro-death pathway in retinal ganglion cells after intraocular pressure elevation
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
desmin + H2O
additional information
-
-
-
two proteolytic fragments of 35000 Da and of 16000 Da
?
additional information
?
-
-
the enzyme might be involved in light-dependent regulation of disk membrane morphogenesis by proteolysis of myosin II
-
-
?
additional information
?
-
-
fetuin A is a potential extracellular regulator of m-calpain at nascent sites of plasma membrane wounding
-
-
?
additional information
?
-
enzyme is involved in cytoskeleton remodelling and signal transduction
-
-
?
additional information
?
-
-
enzyme is involved in cytoskeleton remodelling and signal transduction
-
-
?
additional information
?
-
enzyme is involved in essential cellular functions mediated by calcium. Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit
-
-
?
additional information
?
-
-
enzyme plays a pivotal role during the earlier stages of myogenesis, particularly during fusion. MyoD and myogenin can transactivate capn2, but MyoD shows a higher transactivation level for the regulatory sequences
-
-
?
additional information
?
-
-
calpain mediates calcium-induced activation of the Erk1,2 MAPK pathway and cytoskeletal phosphorylation in neurons
-
-
?
additional information
?
-
pathological conditions associated with the gene of calpain 2: muscular dystrophy, stroke, traumatic brain injury, spinal cord injury, Alzheimer's diseases, Parkinson's disease, neurodegenerative disorders, cataracts, cancer
-
-
?
additional information
?
-
-
pathological conditions associated with the gene of calpain 2: muscular dystrophy, stroke, traumatic brain injury, spinal cord injury, Alzheimer's diseases, Parkinson's disease, neurodegenerative disorders, cataracts, cancer
-
-
?
additional information
?
-
-
calpain 2activity is critical for the life cycle of echovirus 1 and important in the multiplication of the viral RNA genome
-
-
?
additional information
?
-
-
calpain mediates angiogenic effects induced by vascular endothelial growth factor by modulating actin cytoskeletal organization
-
-
?
additional information
?
-
-
calpain-2 plays an important role in lung endothelial cell migration and proliferation
-
-
?
additional information
?
-
-
catalytic activity of calpain is required to limit pseudopod formation in the direction of chemoattractant and for efficient chemotaxis. Calpain 2 is a novel component of the frontness signal that promotes polarization during chemotaxis
-
-
?
additional information
?
-
-
m-calpain activity is triggered by Ca2+-influx and promoted mainly through the phosphatidylinositol 3-kinase pathway
-
-
?
additional information
?
-
-
M-calpain is the major candidate of the proteinase to generate the aggrecan product with the COOH terminal neoepitope VPGVA709 (consisting of two NH2 terminal globular domain G1 and G2 and KS side chains) during the intracellular aggrecan processing
-
-
?
additional information
?
-
-
epidermal growth factor receptor activation of calpain is required for fibroblast motility and occurs via an ERK/MAP kinase signaling pathway
-
-
?
additional information
?
-
-
calpain 2 is likely to be involved with signal transduction events in the lens
-
-
?
additional information
?
-
-
calpain 2 plays a role in limiting membrane protrusion and in regulating lamellipodial dynamics at the leading edge of migrating cells
-
-
?
additional information
?
-
-
functions for nCL-2 involve the membrane trafficking of mucus cells by interacting with coat proteins
-
-
?
additional information
?
-
-
CAPN2 may represent a key factor in development from the first cell division
-
-
?
additional information
?
-
m-calpain is vital for development of the preimplantation murine embryo
-
-
?
additional information
?
-
-
m-calpain is vital for development of the preimplantation murine embryo
-
-
?
additional information
?
-
-
the enzyme is involved in myoblast fusion by cleaving certain proteins. This cleavage could modify membrane and cytoskeleton organization for the myoblast to fuse
-
-
?
additional information
?
-
-
mu-calpain, m-calpain, 20S proteasome, dipeptidyl peptidase II and III and soluble alanyl aminopeptidase are thought to induce lens opacification kinetically during cataract formation in Shumiya cataract rats through the intracellular turnover of lens proteins
-
-
?
additional information
?
-
-
calpain-mediated impairment of Na+/K+-ATPase activity during early reperfusion contributes to cell death after myocardial ischemia
-
-
?
additional information
?
-
-
ERp75 plays important role in the refolding of mitochondrial m-calpain large subunit, cytosolic m-calpain does not associate with ERp57
-
-
?
additional information
?
-
-
representatives of the protein phosphatase 2A subunit classes C, PR65/A, PR55/B and PR61/B' are resistant to m-calpain-mediated degradation
-
-
?
additional information
?
-
enzyme is involved in myofibrillar protein degradation
-
-
?
additional information
?
-
-
enzyme is involved in myofibrillar protein degradation
-
-
?
additional information
?
-
hypoxia upregulates calpain activity and mRNA expression in pulmonary artery endothelial cells
-
-
?
vimentin + H2O
additional information
-
-
-
four proteolytic fragments: 3 major compounds of 54000 Da, 46000 Da and 20000 Da, and a minor fragment of 28000 Da
?
