Literature summary for 3.4.22.53 extracted from

Dutt, P.; Springgs, C.N.; Davies, P.L.; Jia, Z.; Elce, J.S.
Origins of the difference in Ca2+ requirement for activation of mu- and m-calpain (2002), Biochem. J., 367, 263-269.

Search for more literature for 3.4.22.53

Cloned(Commentary)

Cloned (Comment)	Organism
coexpression from large-subunit and small-subunit plasmids in Escherichia coli strain BL21(DE3)	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
additional information	replacement of the five m-calpain residues 517-521, Glu-Ala-Asn-Ile-Glu by the corresponding six mu-calpain residues 528-533, Gln-Ala-Asn-Leu-Pro-Asp, replacement of three m-calpain residues 639-641, Pro-Cys-Gln, by the corresponding three mu-calpain residues 651-653, Asn-Lys-Lys, or replacement of two m-calpain residues 578-579, Lys-Ile by the corresponding mu-calpain residues 590-591, Arg-Ser. Mutations do not affect the expression and Kd values of the resultant calpains. In a series of hybrid mu/m large-subunit calpains, the Kd values decrease progressively towards that of mu-calpain as the portion of mu-type sequence increases from 0 to 90%	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	Kd-value: 0.325 mM. 25% of the difference in Kd values between mu-calpain and m-calpain can be ascribed to the N-terminal peptide of the large subunit, whereas the C-terminal EF-hand-containing domain IV accounts for 65% of the difference	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21000	-	1 * 80000 + 1 * 21000	Rattus norvegicus
80000	-	1 * 80000 + 1 * 21000	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
casein + H2O	-	Rattus norvegicus	?	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 80000 + 1 * 21000	Rattus norvegicus

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Release 2023.1 (January 2023)