Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.53 extracted from

  • Azarian, S.M.; Schlamp, C.L.; Williams, D.S.
    Characterization of calpain II in the retina and photoreceptor outer segments (1993), J. Cell Sci., 105, 787-798.
No PubMed abstract available
Search for more literature for 3.4.22.53

Inhibitors

Inhibitors Comment Organism Structure
calpeptin 50% inhibition of maximal caseinolytic activity at 10 nM and 21 nM for retinal and brain calpain Bos taurus
Ep-475 0.001 mM, 49% inhibition of the enzyme from retina, 46% inhibition of the enzyme from brain Bos taurus
leupeptin 0.001 mM, 67% inhibition of enzyme from retina and brain Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoskeleton
-
 Bos taurus 5856
-
cytosol
-
 Bos taurus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ half maximal activity for retina and brain enzyme is 0.262 mM and 0.311 mM, maximal activity near 1 mM, no activity in presence of 0.03 mM Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus the enzyme might be involved in light-dependent regulation of disk membrane morphogenesis by proteolysis of myosin II ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-
photoreceptor outer segment Bos taurus
-
retina highest activity in plexiform layers and in the photoreceptor outer segments. In dark-adapted retinas the label is distributed throughout the outer segments. In light-adapted retinas, outer segment labelling is concentrated in the connecting cilium and the inner segments are labeled Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-actin + H2O slow degradation Bos taurus ?
-
 ?
casein + H2O
-
 Bos taurus ?
-
 ?
additional information no proteolysis of actin Bos taurus ?
-
 ?
additional information the enzyme might be involved in light-dependent regulation of disk membrane morphogenesis by proteolysis of myosin II Bos taurus ?
-
 ?
myosin II heavy chain + H2O rapid proteolysis Bos taurus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 enzyme from retina and brain Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity, enzyme from retina and brain Bos taurus