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3.4.22.53: calpain-2

This is an abbreviated version!
For detailed information about calpain-2, go to the full flat file.

Word Map on EC 3.4.22.53

Reaction

broad endopeptidase specificity =

Synonyms

Cal II, calcium-activated neutral protease II, calpain 2, calpain II, calpain xCL-2 (Xenopus leavis), calpain-2, calpain-2-like, calpain2, CAPN II, CAPN2, CAPN2 g.p. (Homo sapiens), CPN2, cysteine protease, EC 3.4.22.17, EC 3.4.24.5, human calpain 2, m-calpain, milli-calpain, mitochondrial m-calpain, nCL-2, rat calpain 2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.53 calpain-2

Purification

Purification on EC 3.4.22.53 - calpain-2

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose column chromatography, DEAE-TSK column chromatography, and Reactive Red gel filtration
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ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose column chromatography, Reactive Red column chromatography, and DEAE-TSK anion exchange column chromatography
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DEAE Sephacel gel filtration
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DEAE-Sepharose CL-6B column chromatography
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DEAE-Sepharose column chromatography
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E-F hand structure-domains
HisTrap column chromatography
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large-scale
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m-calpain
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recombinant enzyme
recombinant wild-type and mutant enzymes
single-step purification of calpain-1, calpain-2, and calpastatin using anion-exchange chromatography
wild-type and mutant calpains