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Literature summary for 3.4.22.53 extracted from
- Purification and characterization of a calcium-activated neutral protease from monkey brain and its action on neuropeptides (1984), J. Biochem., 96, 775-784.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Macaca fuscata |  |
| E-64 | - |
Macaca fuscata | |
| leupeptin | - |
Macaca fuscata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requires millimolar order calcium ions for activation | Macaca fuscata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
1 * 74000 + 1 * 20000, SDS-PAGE | Macaca fuscata |
| 74000 | - |
1 * 74000 + 1 * 20000, SDS-PAGE | Macaca fuscata |
| 110000 | - |
gel filtration | Macaca fuscata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Macaca fuscata | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Macaca fuscata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Macaca fuscata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-neoendorphin + H2O | cleavage of the 6Arg-7Lys bond | Macaca fuscata | ? | - |
? | |
| angiotensin + H2O | clevage of the 4tyr-5Ile bond | Macaca fuscata | ? | - |
? | |
| beta-lipotropin(61-91) + H2O | cleavage of the bonds: Thr76-Leu77, Lys84-Asn85 and Lys88-Lys89 | Macaca fuscata | ? | - |
? | |
| beta-neoendorphin + H2O | cleavage of the 6Arg-7Lys bond | Macaca fuscata | ? | - |
? | |
| casein + H2O | - |
Macaca fuscata | ? | - |
? | |
| dynorphin (1-13) + H2O | cleavage of the 6Arg-7Arg bond | Macaca fuscata | ? | - |
? | |
| Leu-enkephalin + H2O | cleaved only slightly at the 1Tyr-2Gly bond | Macaca fuscata | ? | - |
? | |
| Met-enkephalin + H2O | cleaved only slightly at the 1Tyr-2Gly bond | Macaca fuscata | ? | - |
? | |
| neurotensin + H2O | cleavage of the 3Tyr-4Glu bond and the 5Asn-6Lys bond | Macaca fuscata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 74000 + 1 * 20000, SDS-PAGE | Macaca fuscata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Macaca fuscata |
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