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Literature summary for 3.4.22.53 extracted from

  • Inomata, M.; Nomoto, M.; Hayashi, M.; Nakamura, M.; Imahori, K.; Kawashima, S.
    Comparison of low and high calcium requiring forms of the calcium-activated neutral protease (CANP) from rabbit skeletal muscle (1984), J. Biochem., 95, 1661-1670.
    View publication on PubMed
Search for more literature for 3.4.22.53

Inhibitors

Inhibitors Comment Organism Structure
antipain 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
E-64c 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
leupeptin 0.01 mM, 80-90% inhibition Oryctolagus cuniculus
pepstatin A 1 mM, 60-80% inhibition Oryctolagus cuniculus
TLCK 0.1 mM, 60-80% inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 succinyl-bovine serum albumin pH 7.5, 25°C Oryctolagus cuniculus
10
-
 succinyl-casein pH 7.5, 25°C Oryctolagus cuniculus
101.3
-
 succinyl-protamine pH 7.5, 25°C Oryctolagus cuniculus
453.7
-
 succinyl-insulin B pH 7.5, 25°C Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ka-value: 0.7 mM Oryctolagus cuniculus
Sr2+ activates, Ka: 5.1 mM Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-bovine serum albumin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
succinyl-bovine-serum-albumin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
succinyl-casein + H2O
-
 Oryctolagus cuniculus ?
-
 ?
succinyl-insulin B + H2O
-
 Oryctolagus cuniculus ?
-
 ?
succinyl-protamine + H2O
-
 Oryctolagus cuniculus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, complete loss of activity Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Oryctolagus cuniculus