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3.4.22.53: calpain-2

This is an abbreviated version!
For detailed information about calpain-2, go to the full flat file.

Word Map on EC 3.4.22.53

Reaction

broad endopeptidase specificity =

Synonyms

Cal II, calcium-activated neutral protease II, calpain 2, calpain II, calpain xCL-2 (Xenopus leavis), calpain-2, calpain-2-like, calpain2, CAPN II, CAPN2, CAPN2 g.p. (Homo sapiens), CPN2, cysteine protease, EC 3.4.22.17, EC 3.4.24.5, human calpain 2, m-calpain, milli-calpain, mitochondrial m-calpain, nCL-2, rat calpain 2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.53 calpain-2

General Stability

General Stability on EC 3.4.22.53 - calpain-2

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
50% inactivation by autolysis after 1 min at 30°C, pH 7.5, 10 mM Ca2+
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50% inhibition by trypsin after 3 min at 30°C, pH 7.5
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bovine fetuin A stabilizes proteolytic activity of purified m-calpain incubated in the presence of mM calcium chloride and prevents calcium-dependent m-calpain aggregation
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m-calpain loses 50-55% of its proteolytic activity within 5 min during incubation at pH 7.5 in 300 mM or high salt and at a slower rat in 100 mM salt. This loss of activity is not reversed by dialysis for 18 h against a low-ionic-strength buffer at pH 7.5. Proteolytic activity of the unautolyzed calpains is not affected by incubation for 45 min at ionic strength up to 1000 mM. Ionic strengths of 100 mM or above cause dissociation of the two subunits of autolyzed calpains. The dissociated large subunits aggregate to form dimers and trimers, which are proteolytically inactive
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