3.4.22.16: cathepsin H
This is an abbreviated version!
For detailed information about cathepsin H, go to the full flat file.
Reaction
Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-/- bonds) as well as an endopeptidase =
Synonyms
aleurain, aleuron thiol protease, alpha-N-benzoyl-arginine 2-naphthylamide hydrolase, alpha-N-benzoylarginine-beta-naphthylamide hydrolase, Cat H, CatB3, CatH, cathepsin B3, cathepsin Ba, cathepsin H, cathepsin H-like cysteine proteinase, CTSH, procathepsin H
ECTree
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Purification
Purification on EC 3.4.22.16 - cathepsin H
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ammonium sulfate precipitation, CM Sephadex column chromatography, C-5 column chromatography, DEAE Sephadex A-5 column chromatography, and Sephadex G-1 and gel filtration
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ammonium sulfate precipitation, CM-Sephadex column chromatography, C-50 column chromatography, DEAE-Sephadex A-50., and Sephadex G-100, and gel filtration
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native enzyme from liver homogenate by acetone powder preparation, acid autolysis, ammonium sulfate fractionation, gel filtration, and cation exchange and anion exchange chromatography
native enzyme from liver homogenate by acetone powder preparation, acid autolysis, ammonium sulfate fractionation, gel filtration, cation exchange and anion exchange chromatography, and again gel filtration
recombinant His-tagged wild-type and mutant enzymes from Spodoptera frugiperda Sf9 cells or HEK-23T cells, respectively, by nickel affinity chromatography and gel filtration