Information on EC 3.4.22.16 - cathepsin H

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.16
-
RECOMMENDED NAME
GeneOntology No.
cathepsin H
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-/- bonds) as well as an endopeptidase
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
endopeptidase; peptides, endopeptidase
-
CAS REGISTRY NUMBER
COMMENTARY hide
60748-73-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Capra aegagrus hircus
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
up-regulation of catfish cathepsin H and L transcripts during the early stage of Edwardsiella ictaluri infection
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-Ala-4-nitroanilide + H2O
acetyl-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
ir
actin + H2O
?
show the reaction diagram
-
degradation at pH 4.0
-
?
Ala-4-methylcoumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
ir
alpha-N-benzoyl-DL-Arg-beta-naphthylamide + H2O
alpha-N-benzoyl-DL-Arg + beta-naphthylamine
show the reaction diagram
-
-
-
?
alpha-N-benzoyl-DL-arginine-2-naphthylamide + H2O
alpha-N-benzoyl-DL-arginine + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O
alpha-N-benzoyl-DL-arginine + beta-naphthylamine
show the reaction diagram
alpha-neoendorphin + H2O
?
show the reaction diagram
-
preferentially aminopeptidase activity, endoeptidase activity after prolonged incubation
-
?
Arg-2-naphthylamide + H2O
Arg + 2-naphthylamine
show the reaction diagram
Arg-4-methylcoumaryl-7-amide + H2O
Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Arg-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
-
-
-
-
ir
benzoyl-Arg-4-methylcoumaryl-7-amide + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
ir
benzoyl-DL-Arg-2-naphthylamide + H2O
benzoyl-DL-Arg + 2-naphthylamine
show the reaction diagram
benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide + H2O
benzoyl-L-Phe-L-Val-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro + H2O
benzyloxycarbonyl-Pro-Ala-Ala-Ala + Pro
show the reaction diagram
-
-
-
ir
benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro-NH2 + H2O
benzyloxycarbonyl-Pro-Ala-Ala-Ala + Pro-NH2
show the reaction diagram
-
-
-
ir
bone morphogenetic protein 4 + H2O
?
show the reaction diagram
-
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
no aminopeptidase activity, endopeptidase activity after prolonged incubation
-
?
citrulline-4-methylcoumaryl-7-amide + H2O
citrulline + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
ir
demorphin + H2O
?
show the reaction diagram
-
no aminopeptidase activity, endopeptidase activity after prolonged incubation
-
?
dynorphin(1-13) + H2O
?
show the reaction diagram
-
preferentially aminopeptidase activity, endoeptidase activity after prolonged incubation
-
?
dynorphin(1-6) + H2O
?
show the reaction diagram
-
preferentially aminopeptidase activity, endoeptidase activity after prolonged incubation
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
?
Gly-beta-naphthylamide + H2O
Gly + beta-naphthylamine
show the reaction diagram
-
very poor substrate
-
?
H2N-Arg-7-amido-4-methylcoumarin + H2O
H2N-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-Ala-beta-naphthylamide + H2O
L-Ala + beta-naphthylamine
show the reaction diagram
-
-
-
?
L-Arg-4-methylcoumaryl-7-amide + H2O
L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
L-Arg-beta-naphthylamide + H2O
L-Arg + beta-naphthylamine
show the reaction diagram
-
-
-
?
L-Leu-2-naphthylamide + H2O
L-Leu + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
L-Leu-beta-naphthylamide + H2O
L-Leu + beta-naphthylamine
show the reaction diagram
-
-
-
?
L-leucyl-2-naphthylamide + H2O
L-leucine + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
L-Lys-7-amido-4-methylcoumarin + H2O
L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
L-Lys-beta-naphthylamide + H2O
L-Lys + beta-naphthylamine
show the reaction diagram
-
-
-
?
L-Met-beta-naphthylamide + H2O
L-Met + beta-naphthylamine
show the reaction diagram
-
-
-
?
L-Pro-beta-naphthylamide + H2O
L-Pro + beta-naphthylamine
show the reaction diagram
-
very poor substrate
-
?
L-Ser-beta-naphthylamide + H2O
L-Ser + beta-naphthylamine
show the reaction diagram
-
-
-
?
Leu-2-naphthylamide + H2O
Leu + 2-naphthylamine
show the reaction diagram
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
ir
Leu-enkephalin + H2O
?
show the reaction diagram
-
preferentially aminopeptidase activity, endoeptidase activity after prolonged incubation
-
?
Lys-4-methylcoumaryl-7-amide + H2O
Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
ir
Lys-Arg-(Met)enkephalin + H2O
Lys-Arg + (Met)enkephalin
show the reaction diagram
Lys-Bradykinin + H2O
?
show the reaction diagram
-
preferentially aminopeptidase activity, endoeptidase activity after prolonged incubation
-
?
Lys-Lys-(Met)enkephalin + H2O
Lys-Lys + (Met)enkephalin
show the reaction diagram
Met-2-naphthylamide + H2O
Met + 2-naphthylamine
show the reaction diagram
-
189% activity of substrate Leu-2-N-naphthylamine
-
ir
myofibrillar protein + H2O
?
show the reaction diagram
-
-
-
-
?
myosin heavy chain + H2O
?
show the reaction diagram
-
degradation at pH 4.0
-
?
neurotensin + H2O
?
show the reaction diagram
-
no aminopeptidase activity, endopeptidase activity after prolonged incubation
-
?
pro-granzyme B + H2O
granzyme B + ?
show the reaction diagram
-
cathepsin H has pro-granzyme B convertase activity in vitro
-
-
?
pro-surfactant protein B-EGFP fusion protein + H2O
?
show the reaction diagram
-
cleavage 13 amino acids upstream of the NH2-terminus of mature surfactant protein B, and at the boundary between surfactant protein and C-terminal peptide
-
-
?
Proteins + H2O
?
show the reaction diagram
succinyl-Ala-Ala-4-nitroanilide + H2O
succinyl-Ala-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
ir
succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
succinyl-Ala-Ala-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
ir
succinyl-Ala-Ala-Ala-Ala-4-nitroanilide + H2O
succinyl-Ala-Ala-Ala-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
ir
succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide + H2O
succinyl-Ala-Ala-Ala-Ala-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
ir
succinyl-Ala-Ala-Pro-4-nitroanilide + H2O
succinyl-Ala-Ala-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
ir
succinyl-Ala-Pro-Ala-4-nitroanilide + H2O
succinyl-Ala-Pro-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
ir
succinyl-Pro-Ala-Ala-4-nitroanilide + H2O
succinyl-Pro-Ala-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
ir
surfactant protein B + H2O
?
show the reaction diagram
-
-
-
-
?
talin + H2O
?
show the reaction diagram
-
cathepsin H cleaves the N-terminus of the talin F0 head domain
-
-
?
tropomyosin + H2O
?
show the reaction diagram
-
degradation at pH 4.0
-
?
troponin I + H2O
?
show the reaction diagram
-
degradation at pH 4.0, little degradation at pH 5.o-5.6
-
?
VALSLKISIGNVVKTMQFEPST + H2O
?
show the reaction diagram
-
the peptide mimicks the N-terminus of the talin F0 head domain
-
-
?
Z-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
?
show the reaction diagram
-
no substrate for wild type enzyme, endopeptidase activity of mutant T11_E18del-cathepsin H
-
?
Z-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
benzoyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Fibronectin + H2O
?
show the reaction diagram
Q86MW6
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
Q86MW6
-
-
-
?
Proteins + H2O
?
show the reaction diagram
surfactant protein B + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme is involved in maturation of the biologically active surfactant protein B
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S)-2-amino-N-[(1S)-2-(biphenyl-4-yl)-1-cyanoethyl]butanamide
-
-
1,10-phenanthroline
1,3,5-triphenyl pyrazole
-
-
-
1,3,5-triphenyl-2-pyrazoline
-
-
-
1H-spiro(cyclohexane-1,2-quinazolin)-4(3H)-one
-
competitive inhibition, 94.86% residual activity at 0.1 mM
-
2,2'-dipyridyl disulfide
2-(4-(dimethylamino)phenyl)-2,3-dihydroquinazolin-4(1H)-one
-
competitive inhibition, 88.37% residual activity at 0.1 mM
-
2-(4-bromophenyl)-2,3-dihydroquinazolin-4(1H)-one
-
competitive inhibition, 91.62% residual activity at 0.1 mM
-
2-(4-chlorophenyl)-2,3-dihydroquinazolin-4(1H)-one
-
competitive inhibition, 98.11% residual activity at 0.1 mM
-
2-(4-fluorophenyl)-2,3-dihydroquinazolin-4(1H)-one
-
competitive inhibition, 94.05% residual activity at 0.1 mM
-
2-(4-methoxyphenyl)-2,3-dihydroquinazolin-4(1H)-one
-
competitive inhibition, 95.94% residual activity at 0.1 mM
-
2-(4-methylphenyl)-2,3-dihydroquinazolin-4(1H)-one
-
competitive inhibition, 39.18% residual activity at 0.1 mM
-
2-(4-nitrophenyl)-2,3-dihydroquinazolin-4(1H)-one
-
competitive inhibition, 81.89% residual activity at 0.1 mM
-
2-chlorobenzohydrazide
-
non-competitive inhibition; non-competitive inhibition, 89.46% residual activity at 0.01 mM
2-methoxybenzohydrazide
-
non-competitive inhibition; non-competitive inhibition, 97.83% residual activity at 0.01 mM
2-phenyl-2,3-dihydroquinazolin-4(1H)-one
-
competitive inhibition, 79.73% residual activity at 0.1 mM
3,5-diphenyl-2-pyrazoline
-
-
-
3,5-diphenyl-4-amino-1,2,4-triazole
-
non-competitive inhibition; non-competitive inhibition, 69.73% residual activity at 0.01 mM
3-(2'-hydroxy-3'-methylphenyl)-5-(2'-hydroxy-3'-methylphenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition, 88.11% residual activity at 0.01 mM
-
3-(2'-methylphenyl)-5-(2'-methylphenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition, 82.97% residual activity at 0.01 mM
-
3-(2-hydroxy-3-methylphenyl)-5-(2-hydroxy-3-methylphenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition
3-(2-methylphenyl)-5-(2-methylphenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition
3-(3'-aminophenyl)-5-(3'-aminophenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition, 16.37% residual activity at 0.01 mM
-
3-(3'-methylphenyl)-5-(3'-methylphenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition, 79.45% residual activity at 0.01 mM
-
3-(3'-nitrophenyl)-5-(3'-nitrophenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition, 45.13% residual activity at 0.01 mM
-
3-(3-aminophenyl)-5-(3-aminophenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition
3-(3-methylphenyl)-5-(3-methylphenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition
3-(4'-chlorophenyl)-5-(4'-chlorophenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition, 24.35% residual activity at 0.01 mM
-
3-(4'-methylphenyl)-5-(4'-methylphenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition, 89.46% residual activity at 0.01 mM
-
3-(4-chlorophenyl)-5-(4-chlorophenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition
3-(4-methylphenyl)-5-(4-methylphenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition
3-phenyl-5-(3-nitrophenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition
3-phenyl-5-(4'-nitrophenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition, 78.11% residual activity at 0.01 mM
-
3-phenyl-5-(4-nitrophenyl)-4-amino-1,2,4-triazole
-
non-competitive inhibition
4-aminophenylmercuric acetate
-
93% inhibition at 1 mM
4-bromochalcone phenyl hydrazone
-
-
-
4-chlorochalcone phenyl hydrazone
-
-
-
4-hydroxybenzohydrazide
-
non-competitive inhibition; non-competitive inhibition, 91.62% residual activity at 0.01 mM
4-methoxybenzohydrazide
-
non-competitive inhibition; non-competitive inhibition, 45% residual activity at 0.01 mM
4-methoxychalcone phenyl hydrazone
-
-
-
4-methylchalcone phenyl hydrazone
-
-
-
4-nitrochalcone phenyl hydrazone
-
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
95% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine
5-(4-bromophenyl)-1,3-diphenyl pyrazole
-
-
-
5-(4-bromophenyl)-1,3-diphenyl-2-pyrazoline
-
-
-
5-(4-bromophenyl)-3-phenyl-2-pyrazoline
-
-
-
5-(4-chlorophenyl)-1,3-diphenyl pyrazole
-
-
-
5-(4-chlorophenyl)-1,3-diphenyl-2-pyrazoline
-
-
-
5-(4-chlorophenyl)-3-phenyl-2-pyrazoline
-
-
-
5-(4-methoxyphenyl)-1,3-diphenyl pyrazole
-
-
5-(4-methoxyphenyl)-1,3-diphenyl-2-pyrazoline
-
-
5-(4-methoxyphenyl)-3-phenyl-2-pyrazoline
-
-
5-(4-methylphenyl)-1,3-diphenyl pyrazole
-
-
5-(4-methylphenyl)-1,3-diphenyl-2-pyrazoline
-
-
5-(4-methylphenyl)-3-phenyl-2-pyrazoline
-
-
5-(4-nitrophenyl)-1,3-diphenyl pyrazole
-
-
5-(4-nitrophenyl)-1,3-diphenyl-2-pyrazoline
-
-
5-(4-nitrophenyl)-3-phenyl-2-pyrazoline
-
-
acetyl-(Ala)2-Pro-Ala-CH2Cl
-
100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine
acetyl-(Ala)2-Pro-Val-CH2Cl
-
100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine
acetyl-(Ala)4-CH2Cl
-
100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine
alpha-2-Macroglobulin
-
-
-
antipain
benzyloxycarbonyl-Gly-Leu-NH
-
inhibits processing of proenzyme to active form
Benzyloxycarbonyl-Leu-NH2
-
inhibits formation of active enzyme from procathepsin H
Benzyloxycarbonyl-Phe-Phe-CHN2
-
31% inhibition at 0.01 mM
bestatin
-
62% inhibition at 1 mM, substrate Leu-2-N-naphthylamine
bovine stefin B
-
-
-
CdCl2
-
90% inhibition at 1 mM
chalcone phenyl hydrazone
-
-
chicken cystatin
-
chicken egg white cystatin
-
-
-
chymostatin B
-
47% inhibition at 0.016 mM
-
CNWAAGYNCGGGS-NH2
-
cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain
CNWTLGGYKCGGGS-NH2
-
cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain
CoCl2
-
100% inhibition at 1 mM
CPI-H
-
peptide inhibitor of 13 kDa from rabbit skeletal muscle, noncompetitive
-
CPI-L
-
peptide inhibitor of 23 kDa from rabbit skeletal muscle, noncompetitive
-
CuCl2
-
94% inhibition at 1 mM
CWEWGGWHCGGSS-OH
-
cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain
CWSMMGFQCGGGS-NH2
-
cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain
Cystatin
-
cystatin C
-
cystatin C, full-length
-
-
-
cystatin C, truncated form
-
deletion of 10 N-terminal amino acids
-
decyl bromide
-
100% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine
DTNB
-
100% inhibition at 1 mM
E-64
-
irreversible active site inhibitor
ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate
H2N-Ser(O-Bzl)-CHN2
-
-
HgCl2
Human cystatin C
-
Ki: 0.01-0.03 nM
-
human stefin A
-
human stefin B
-
Hyamine 1622
-
52% inhibition at 1 mM
iodoacetamide
iodoacetic acid
Leu-CH2-Cl
-
complete inhibition at 0.01 mM
Leu-CH2Cl
leupeptin
MnSO4
-
64% inhibition at 1 mM, substrate Leu-2-N-naphthylamine
N-acetyl-3,5-diphenyl pyrazolines
-
-
N-acetyl-5-(4-bromophenyl)-3-phenyl-2-pyrazoline
-
-
N-acetyl-5-(4-chlorophenyl)-3-phenyl-2-pyrazoline
-
-
N-acetyl-5-(4-methoxyphenyl)-3-phenyl-2-pyrazoline
-
-
N-acetyl-5-(4-methylphenyl)-3-phenyl-2-pyrazoline
-
-
N-acetyl-5-(4-nitrophenyl)-3-phenyl-2-pyrazoline
-
-
N-benzoyl-3,5-diphenylpyrazoline
-
; 66.19% residual activity at 0.01 mM
N-benzoyl-5-(2-chloro phenyl)-3-phenylpyrazoline
-
-
N-benzoyl-5-(2-chlorophenyl)-3-phenylpyrazoline
-
55.13% residual activity at 0.01 mM
N-benzoyl-5-(2-methoxyphenyl)-3-phenylpyrazoline
-
; 79.37% residual activity at 0.01 mM
N-benzoyl-5-(2-nitrophenyl)-3-phenylpyrazoline
-
; 34.77% residual activity at 0.01 mM
N-benzoyl-5-(3-chloro phenyl)-3-phenylpyrazoline
-
-
N-benzoyl-5-(3-chlorophenyl)-3-phenylpyrazoline
-
44.2% residual activity at 0.01 mM
N-benzoyl-5-(3-methoxy phenyl)-3-phenylpyrazoline
-
-
N-benzoyl-5-(3-methoxyphenyl)-3-phenylpyrazoline
-
76.06% residual activity at 0.01 mM
N-benzoyl-5-(3-nitrophenyl)-3-phenylpyrazoline
-
; 27.49% residual activity at 0.01 mM
N-benzoyl-5-(4-chloro phenyl)-3-phenylpyrazoline
-
-
N-benzoyl-5-(4-chlorophenyl)-3-phenylpyrazoline
-
62.47% residual activity at 0.01 mM
N-benzoyl-5-(4-methoxy phenyl)-3-phenylpyrazoline
-
-
N-benzoyl-5-(4-methoxyphenyl)-3-phenylpyrazoline
-
69.69% residual activity at 0.01 mM
N-benzoyl-5-(4-nitrophenyl)-3-phenylpyrazoline
-
; 28.77% residual activity at 0.01 mM
N-ethylmaleimide
N-formyl-3,5-diphenylpyrazoline
-
; 76.78% residual activity at 10 mM
N-formyl-5-(2-chlorophenyl)-3-phenylpyrazoline
-
; 61.38% residual activity at 10 mM
N-formyl-5-(2-methoxyphenyl)-3-phenylpyrazoline
-
; 92.97% residual activity at 0.001 mM
N-formyl-5-(2-nitrophenyl)-3-phenylpyrazoline
-
; 30.2% residual activity at 0.001 mM
N-formyl-5-(3-chlorophenyl)-3-phenylpyrazoline
-
; 49.57% residual activity at 0.001 mM
N-formyl-5-(3-methoxyphenyl)-3-phenylpyrazoline
-
; 86.03% residual activity at 0.001 mM
N-formyl-5-(3-nitrophenyl)-3-phenylpyrazoline
-
; 14.15% residual activity at 0.001 mM
N-formyl-5-(4-chlorophenyl)-3-phenylpyrazoline
-
; 69.38% residual activity at 0.001 mM
N-formyl-5-(4-methoxyphenyl)-3-phenylpyrazoline
-
; 80.06% residual activity at 0.001 mM
N-formyl-5-(4-nitrophenyl)-3-phenylpyrazoline
-
; 27.64% residual activity at 0.001 mM
N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-norvalinamide
-
-
N2-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-leucinamide
-
-
p-Hydroxymercuriphenylsulfonic acid
-
83% inhibition at 0.011 mM, substrate Leu-2-N-naphthylamine
PCMB
-
46% inhibition at 1 mM
phenylacetyl hydrazine
-
non-competitive inhibition; non-competitive inhibition, 81.08% residual activity at 0.01 mM
Proteinase inhibitor
-
puromycin
Soybean trypsin inhibitor
-
Thiol proteinase inhibitor
-
thiol-blocking agents
tosyl-Leu-CH2Cl
tosyl-Phe-CH2Cl
Zn(CH3COO)2
-
85% inhibition at 1 mM
ZnSO4
-
87% inhibition at 1 mM, substrate Leu-2-N-naphthylamine
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
cysteine
dithioerythritol
-
activates, synergistic with EDTA
dithiothreitol
glutathione
thioglycolic acid
-
activates, synergistic with EDTA
thiol compounds
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
210
Ala-4-methylcoumaryl-7-amide
-
-
0.367
alpha-N-benzoyl-DL-Arg-beta-naphthylamide
-
pH 6.2, 37°C
0.097 - 0.169
Arg-2-naphthylamide
0.097 - 72
Arg-4-methylcoumaryl-7-amide
0.637
Benzoyl-Arg-2-naphthylamide
0.321
benzoyl-Arg-4-methylcoumaryl-7-amide
-
at 30°C
0.039 - 0.05
benzoyl-DL-Arg-2-naphthylamide
0.46
benzoyl-L-Arg-2-naphthylamide
-
-
0.082
benzoyl-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
pH 6.5, 25°C, mutant lacking the mini chain
0.025 - 0.038
benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide
7.4
benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro
-
-
3.5
benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro-NH2
-
-
0.076
citrulline-4-methylcoumaryl-7-amide
-
at 30°C
0.05 - 3.6
H-L-Arg-NH-4-methylcoumaryl-7-amide
0.1
L-Arg-4-methylcoumaryl-7-amide
-
pH 6.5, 25°C, native enzyme
0.203
L-Leu-beta-naphthylamide
-
pH 6.2, 37°C
0.167 - 0.22
Leu-2-naphthylamide
35.4
Leu-4-methylcoumaryl-7-amide
-
-
184
Lys-4-methylcoumaryl-7-amide
-
-
0.128
Met-2-naphthylamide
-
-
10.2
succinyl-Ala-Ala-4-nitroanilide
-
-
7
succinyl-Ala-Ala-Ala-4-nitroanilide
-
-
0.7
succinyl-Ala-Ala-Ala-Ala-4-nitroanilide
-
-
0.7
succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide
-
-
27.8
succinyl-Ala-Ala-Pro-4-nitroanilide
-
-
3
succinyl-Ala-Pro-Ala-4-nitroanilide
-
-
2.9
succinyl-Pro-Ala-Ala-4-nitroanilide
-
-
0.092
Z-L-Phe-L-Arg-NH-4-methylcoumaryl-7-amide
-
mutant T11_E18del-cathepsin H, pH 6.8, 30°C
additional information
additional information
-
pH and temperature dependence of kcat/Km
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.87
Ala-4-methylcoumaryl-7-amide
Cyprinus carpio
-
-
1.82 - 115
Arg-2-naphthylamide
2.2 - 8.13
Arg-4-methylcoumaryl-7-amide
1.82
Benzoyl-Arg-2-naphthylamide
Homo sapiens
-
-
0.63
benzoyl-Arg-4-methylcoumaryl-7-amide
Rattus norvegicus
-
at 30°C
5.3
benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro
Rattus norvegicus
-
-
7.7
benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro-NH2
Rattus norvegicus
-
-
3.1
citrulline-4-methylcoumaryl-7-amide
Rattus norvegicus
-
at 30°C
122
Leu-2-naphthylamide
Bos taurus
-
-
1.1
Leu-4-methylcoumaryl-7-amide
Cyprinus carpio
-
-
4.13
Lys-4-methylcoumaryl-7-amide
Cyprinus carpio
-
-
219
Met-2-naphthylamide
Bos taurus
-
-
0.07
succinyl-Ala-Ala-4-nitroanilide
Rattus norvegicus
-
-
0.3
succinyl-Ala-Ala-Ala-4-nitroanilide
Rattus norvegicus
-
-
0.9
succinyl-Ala-Ala-Ala-Ala-4-nitroanilide
Rattus norvegicus
-
-
0.8
succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide
Rattus norvegicus
-
-
0.004
succinyl-Ala-Ala-Pro-4-nitroanilide
Rattus norvegicus
-
-
0.15
succinyl-Pro-Ala-Ala-4-nitroanilide
Rattus norvegicus
-
-
additional information
additional information
Rattus norvegicus
-
pH and temperature dependence of kcat/Km
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0037
(2S)-2-amino-N-[(1S)-2-(biphenyl-4-yl)-1-cyanoethyl]butanamide
-
-
0.01859
1,3,5-triphenyl pyrazole
-
pH 7, 37°C
-
0.005388
1,3,5-triphenyl-2-pyrazoline
-
pH 7, 37°C
-
0.09009
1H-spiro(cyclohexane-1,2-quinazolin)-4(3H)-one
-
at pH 7.0 and 37°C
-
0.01858
2-(4-(dimethylamino)phenyl)-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 37°C
-
0.05263
2-(4-bromophenyl)-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 37°C
-
0.02325
2-(4-chlorophenyl)-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 37°C
-
0.00818
2-(4-fluorophenyl)-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 37°C
-
0.05682
2-(4-methoxyphenyl)-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 37°C
-
0.0025
2-(4-methylphenyl)-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 37°C
-
0.01042
2-(4-nitrophenyl)-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 37°C
-
0.0901
2-chlorobenzohydrazide
-
at pH 7.0 and 37°C; pH 5, 37°C
0.588
2-methoxybenzohydrazide
-
at pH 7.0 and 37°C; pH 5, 37°C
0.01441
2-phenyl-2,3-dihydroquinazolin-4(1H)-one
-
at pH 7.0 and 37°C
0.006024
3,5-diphenyl-2-pyrazoline
-
pH 7, 37°C
-
0.0232
3,5-diphenyl-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C; pH 5, 37°C
0.0781
3-(2'-hydroxy-3'-methylphenyl)-5-(2'-hydroxy-3'-methylphenyl)-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C
-
0.0532
3-(2'-methylphenyl)-5-(2'-methylphenyl)-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C
-
0.0781
3-(2-hydroxy-3-methylphenyl)-5-(2-hydroxy-3-methylphenyl)-4-amino-1,2,4-triazole
-
pH 5, 37°C
0.0532
3-(2-methylphenyl)-5-(2-methylphenyl)-4-amino-1,2,4-triazole
-
pH 5, 37°C
0.002
3-(3'-aminophenyl)-5-(3'-aminophenyl)-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C
-
0.0387
3-(3'-methylphenyl)-5-(3'-methylphenyl)-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C
-
0.0086
3-(3'-nitrophenyl)-5-(3'-nitrophenyl)-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C
-
0.002
3-(3-aminophenyl)-5-(3-aminophenyl)-4-amino-1,2,4-triazole
-
pH 5, 37°C
0.0387
3-(3-methylphenyl)-5-(3-methylphenyl)-4-amino-1,2,4-triazole
-
pH 5, 37°C
0.0033
3-(4'-chlorophenyl)-5-(4'-chlorophenyl)-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C
-
0.0901
3-(4'-methylphenyl)-5-(4'-methylphenyl)-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C
-
0.0033
3-(4-chlorophenyl)-5-(4-chlorophenyl)-4-amino-1,2,4-triazole
-
pH 5, 37°C
0.0901
3-(4-methylphenyl)-5-(4-methylphenyl)-4-amino-1,2,4-triazole
-
pH 5, 37°C
0.0086
3-phenyl-5-(3-nitrophenyl)-4-amino-1,2,4-triazole
-
pH 5, 37°C
0.0364
3-phenyl-5-(4'-nitrophenyl)-4-amino-1,2,4-triazole
-
at pH 7.0 and 37°C
-
0.0364
3-phenyl-5-(4-nitrophenyl)-4-amino-1,2,4-triazole
-
pH 5, 37°C
0.0026
4-bromochalcone phenyl hydrazone
-
pH 7, 37°C
-
0.0011
4-chlorochalcone phenyl hydrazone
-
pH 7, 37°C
-
0.1123
4-hydroxybenzohydrazide
-
at pH 7.0 and 37°C; pH 5, 37°C
0.0082
4-methoxybenzohydrazide
-
at pH 7.0 and 37°C; pH 5, 37°C
0.001695
4-methoxychalcone phenyl hydrazone
-
pH 7, 37°C
-
0.0029
4-methylchalcone phenyl hydrazone
-
pH 7, 37°C
-
0.001905
4-nitrochalcone phenyl hydrazone
-
pH 7, 37°C
-
0.0038
5-(4-bromophenyl)-1,3-diphenyl pyrazole
-
pH 7, 37°C
-
0.002907
5-(4-bromophenyl)-1,3-diphenyl-2-pyrazoline
-
pH 7, 37°C
-
0.00537
5-(4-bromophenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
-
0.002346
5-(4-chlorophenyl)-1,3-diphenyl pyrazole
-
pH 7, 37°C
-
0.001174
5-(4-chlorophenyl)-1,3-diphenyl-2-pyrazoline
-
pH 7, 37°C
-
0.001568
5-(4-chlorophenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
-
0.0033
5-(4-methoxyphenyl)-1,3-diphenyl pyrazole
-
pH 7, 37°C
0.002119
5-(4-methoxyphenyl)-1,3-diphenyl-2-pyrazoline
-
pH 7, 37°C
0.0098
5-(4-methoxyphenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
0.007396
5-(4-methylphenyl)-1,3-diphenyl pyrazole
-
pH 7, 37°C
0.004184
5-(4-methylphenyl)-1,3-diphenyl-2-pyrazoline
-
pH 7, 37°C
0.0082
5-(4-methylphenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
0.00257
5-(4-nitrophenyl)-1,3-diphenyl pyrazole
-
pH 7, 37°C
0.003158
5-(4-nitrophenyl)-1,3-diphenyl-2-pyrazoline
-
pH 7, 37°C
0.0033
5-(4-nitrophenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
0.00000052
bovine stefin B
-
pH 6.5, 25°C, native enzyme
-
0.0048
chalcone phenyl hydrazone
-
pH 7, 37°C
0.000000049
chicken cystatin
-
pH 6.5, 25°C, mutant lacking the mini chain
-
0.000000095 - 0.00000053
chicken egg white cystatin
-
0.0179
CNWAAGYNCGGGS-NH2
-
pH 6.8, 22°C
0.0051
CNWTLGGYKCGGGS-NH2
-
pH 6.8, 22°C
0.00000033
CPI-H
-
pH 6.2, 30°C
-
0.0000005
CPI-L
-
pH 6.2, 30°C
-
0.0049
CWEWGGWHCGGSS-OH
-
pH 6.8, 22°C
0.0039
CWSMMGFQCGGGS-NH2
-
pH 6.8, 22°C
0.000000071
cystatin C, full-length
-
25°C, pH 7.0
-
0.00000032
cystatin C, truncated form
-
25°C, pH 7.0
-
0.000000099
human stefin A
-
pH 6.5, 25°C, mutant lacking the mini chain
-
0.000000074
human stefin B
-
pH 6.5, 25°C, mutant lacking the mini chain
-
0.005
leupeptin
-
-
0.01767
N-acetyl-3,5-diphenyl pyrazolines
-
pH 7, 37°C
0.01266
N-acetyl-5-(4-bromophenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
0.01
N-acetyl-5-(4-chlorophenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
0.14
N-acetyl-5-(4-methoxyphenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
0.03546
N-acetyl-5-(4-methylphenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
0.02432
N-acetyl-5-(4-nitrophenyl)-3-phenyl-2-pyrazoline
-
pH 7, 37°C
0.0032
N-benzoyl-3,5-diphenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.00189
N-benzoyl-5-(2-chloro phenyl)-3-phenylpyrazoline
-
pH 7, 37°C
0.00189
N-benzoyl-5-(2-chlorophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C
0.00965
N-benzoyl-5-(2-methoxyphenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.00144
N-benzoyl-5-(2-nitrophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.00181
N-benzoyl-5-(3-chloro phenyl)-3-phenylpyrazoline
-
pH 7, 37°C
0.00181
N-benzoyl-5-(3-chlorophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C
0.00503
N-benzoyl-5-(3-methoxy phenyl)-3-phenylpyrazoline
-
pH 7, 37°C
0.00503
N-benzoyl-5-(3-methoxyphenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C
0.00098
N-benzoyl-5-(3-nitrophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.00301
N-benzoyl-5-(4-chloro phenyl)-3-phenylpyrazoline
-
pH 7, 37°C
0.00301
N-benzoyl-5-(4-chlorophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C
0.0041
N-benzoyl-5-(4-methoxy phenyl)-3-phenylpyrazoline
-
pH 7, 37°C
0.0041
N-benzoyl-5-(4-methoxyphenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C
0.00127
N-benzoyl-5-(4-nitrophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.000174
N-formyl-3,5-diphenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.000135
N-formyl-5-(2-chlorophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.000387
N-formyl-5-(2-methoxyphenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.0000963
N-formyl-5-(2-nitrophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.00013
N-formyl-5-(3-chlorophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.000272
N-formyl-5-(3-methoxyphenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.0000519
N-formyl-5-(3-nitrophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.000137
N-formyl-5-(4-chlorophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.000221
N-formyl-5-(4-methoxyphenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.000058
N-formyl-5-(4-nitrophenyl)-3-phenylpyrazoline
-
in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C
0.00014
N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-norvalinamide
-
-
0.0014
N2-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-leucinamide
-
-
0.0425
phenylacetyl hydrazine
-
at pH 7.0 and 37°C; pH 5, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000109
-
-
0.0088
-
pH 6.2, 37°C
0.02256
0.02291
-
pH 7, 37°C
0.15
-
substrate benzyloxycarbonyl-Arg-Arg-2-naphthylamide
1.73
-
substrate benzoyl-DL-Arg-2-naphthylamide
1.95
-
substrate Leu-2-naphthylamide
3.5
-
substrate Arg-2-naphthylamide
286
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
assay at
6.5 - 7.2
6.6
-
substrate alpha-N-benzoyl-DL-Arg-beta-naphthylamide
7.2
-
substrate Leu-2-naphthylamide
7.5
-
isoform 2
8
-
or higher, substrate benzoyl-Arg-4-methylcoumaryl-7-amide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
less than 20% of maximal activity above and below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
substrate Arg-4-methylcoumaryl-7-amide
45 - 50
-
substrate-dependent
49
-
substrate benzoyl-Arg 2-naphthylamide
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 6.8
-
and minor band at 6.1, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression level in intestine and gill, but barely in other tissues
Manually annotated by BRENDA team
-
cultured
Manually annotated by BRENDA team
high expression level in intestine and gill, but barely in other tissues
Manually annotated by BRENDA team
cathepsin B3 is detected in the whole body extract of metacercariae and newly excysted juveniles but not in 4-week-old juveniles or adult parasites
Manually annotated by BRENDA team
-
cathepsin H is highly expressed in the secretory organelles of alveolar type II pneumocytes
Manually annotated by BRENDA team
-
inflammated
Manually annotated by BRENDA team
-
683 ng /mg protein, distribution in tissues and peripheral blood cells
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
-
1 * 17000, 1 * 5000, gel filtration after reduction
23000
-
gel filtration
24000
-
1 * 24000, 1 * 4000, SDS-PAGE, partial
25100
-
estimated from sequence of cDNA
27900
mature enzyme, calculated from amino acid sequence
29000
-
gel filtration
34000
native cathepsin B3, SDS-PAGE
35000
-
procathepsin H, estimated from sequence of cDNA
36600
x * 36600, calculated
37100
-
preprocathepsin H, estimated from sequence of cDNA
38000 - 39000
deglycosylation of recombinant CatB3 with endoglycosidase H under denaturing and native conditions produces protein migrating at 38000-39000 Da
39200
proenzyme, calculated from amino acid sequence
55000 - 75000
purified recombinant glycosylated proCatB3 forms migrate at approximately 55000-75000Da
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
side-chain modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
comparison of structure and primary sequences of 5 papain, actinidin, cathepsins B and H and stem bromelain yields high degree of similarity
-
general and secondary structure, structure of active-site cleft, mechanistic conclusions drawn from crystallographic study, mini-chain attached to active-site cleft of enzyme body via disulfide bond, structural comparison reveals high similarity to actinidin
-
in complex with human stefin A
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7.5
-
-
638838
5 - 9
-
-
638840
5.5 - 6
-
rapid inactivation above
638839
7
-
irreversible inactivation above
36634, 638831
8.2
-
stable at 25°C, unstable at 37°C
638825
8.5
-
83% activity
638833
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
rapid inactivation above, without substrate bound
60
-
30 min, 80% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
effects of endoglycosidase H on proenzyme stability
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, goat muscle, 120 days, remains stable with 84-94% residual activity
-
-20°C, 0.02 M sodium acetate buffer, pH 5.2, 1 mM EDTA, several months without loss of activity
-
4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA
4°C or -20°C, 50-100 mM Na-acetate buffer, pH 5.5, 1 mM Na2-EDTA, 0.1% Brij 35, 5 mM Na-tetrathionate, 1 month, 0-10% loss of activity
-
4°C, 0.02 M Na-acetate buffer, pH 5.0, 0.15 mM NaCl
-
4°C, 50 mM Na acetate buffer, pH 4.6, 1 mM EDTA, 1 month, concentrated preparations, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 methods
-
ammonium sulfate precipitation, CM Sephadex column chromatography, C-5 column chromatography, DEAE Sephadex A-5 column chromatography, and Sephadex G-1 and gel filtration
-
ammonium sulfate precipitation, CM-Sephadex column chromatography, C-50 column chromatography, DEAE-Sephadex A-50., and Sephadex G-100, and gel filtration
-
Ni2+-NTA column chromatography
S-carboxymethylated-papain-Sepharose affinity chromatography
-
two methods
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
closely related to rat cathepsin H
-
expressed in Pichia pastoris
sequence comparison between rat and human gene and protein
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine