Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-2-Macroglobulin | - |
Homo sapiens | |
ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate | E-64-d, inhibits processing of proenzyme to synthetic analogs | Homo sapiens | |
ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate | - |
Rattus norvegicus | |
leupeptin | low sensitivity | Homo sapiens | |
leupeptin | Ki: 0.005 mM; low sensitivity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
Arg-2-naphthylamide | - |
Homo sapiens | |
0.15 | - |
Arg-4-methylcoumaryl-7-amide | - |
Homo sapiens | |
0.637 | - |
benzoyl-Arg-2-naphthylamide | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Homo sapiens |
28000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Proteins + H2O | Homo sapiens | aminopeptidase and endopeptidase activity | ? | - |
? | |
Proteins + H2O | Rattus norvegicus | aminopeptidase and endopeptidase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | - |
Homo sapiens |
side-chain modification | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
2 methods | Homo sapiens |
two methods | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA | Homo sapiens |
4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O | - |
Homo sapiens | alpha-N-benzoyl-DL-arginine + beta-naphthylamine | - |
? | |
Arg-2-naphthylamide + H2O | - |
Homo sapiens | Arg + 2-naphthylamine | - |
ir | |
Arg-2-naphthylamide + H2O | - |
Rattus norvegicus | Arg + 2-naphthylamine | - |
ir | |
Arg-4-methylcoumaryl-7-amide + H2O | conventional test substrate | Homo sapiens | Arg + 7-amino-4-methylcoumarin | - |
ir | |
Arg-4-methylcoumaryl-7-amide + H2O | conventional test substrate | Rattus norvegicus | Arg + 7-amino-4-methylcoumarin | - |
ir | |
benzoyl-DL-Arg-2-naphthylamide + H2O | - |
Homo sapiens | benzoyl-DL-Arg + 2-naphthylamine | - |
ir | |
benzoyl-DL-Arg-2-naphthylamide + H2O | - |
Rattus norvegicus | benzoyl-DL-Arg + 2-naphthylamine | - |
ir | |
Leu-2-naphthylamide + H2O | - |
Homo sapiens | Leu + 2-naphthylamine | - |
ir | |
Leu-2-naphthylamide + H2O | - |
Rattus norvegicus | Leu + 2-naphthylamine | - |
ir | |
additional information | activity as endopeptidase and aminopeptidase on synthetic substrates | Homo sapiens | ? | - |
? | |
additional information | activity as endopeptidase and aminopeptidase on synthetic substrates | Rattus norvegicus | ? | - |
? | |
Proteins + H2O | aminopeptidase and endopeptidase activity | Homo sapiens | ? | - |
? | |
Proteins + H2O | aminopeptidase and endopeptidase activity | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
monomer | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Homo sapiens |
40 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.82 | - |
Arg-2-naphthylamide | - |
Homo sapiens | |
1.82 | - |
benzoyl-Arg-2-naphthylamide | - |
Homo sapiens | |
2.53 | - |
Arg-4-methylcoumaryl-7-amide | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | substrate azocasein | Homo sapiens |
5 | 6 | substrate azocasein | Rattus norvegicus |
6.8 | - |
- |
Rattus norvegicus |
6.8 | - |
synthetic substrates | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
irreversible inactivation above | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
leupeptin | - |
Rattus norvegicus |