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Literature summary for 3.4.22.16 extracted from

  • Barrett, A.J.; Kirschke, H.
    Cathepsin B, Cathepsin H, and cathepsin L (1981), Methods Enzymol., 80, 535-561.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
alpha-2-Macroglobulin
-
Homo sapiens
ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate E-64-d, inhibits processing of proenzyme to synthetic analogs Homo sapiens
ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate
-
Rattus norvegicus
leupeptin low sensitivity Homo sapiens
leupeptin Ki: 0.005 mM; low sensitivity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
Arg-2-naphthylamide
-
Homo sapiens
0.15
-
Arg-4-methylcoumaryl-7-amide
-
Homo sapiens
0.637
-
benzoyl-Arg-2-naphthylamide
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Homo sapiens
28000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins + H2O Homo sapiens aminopeptidase and endopeptidase activity ?
-
?
Proteins + H2O Rattus norvegicus aminopeptidase and endopeptidase activity ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification
-
Homo sapiens
side-chain modification
-
Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
2 methods Homo sapiens
two methods Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA Homo sapiens
4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O
-
Homo sapiens alpha-N-benzoyl-DL-arginine + beta-naphthylamine
-
?
Arg-2-naphthylamide + H2O
-
Homo sapiens Arg + 2-naphthylamine
-
ir
Arg-2-naphthylamide + H2O
-
Rattus norvegicus Arg + 2-naphthylamine
-
ir
Arg-4-methylcoumaryl-7-amide + H2O conventional test substrate Homo sapiens Arg + 7-amino-4-methylcoumarin
-
ir
Arg-4-methylcoumaryl-7-amide + H2O conventional test substrate Rattus norvegicus Arg + 7-amino-4-methylcoumarin
-
ir
benzoyl-DL-Arg-2-naphthylamide + H2O
-
Homo sapiens benzoyl-DL-Arg + 2-naphthylamine
-
ir
benzoyl-DL-Arg-2-naphthylamide + H2O
-
Rattus norvegicus benzoyl-DL-Arg + 2-naphthylamine
-
ir
Leu-2-naphthylamide + H2O
-
Homo sapiens Leu + 2-naphthylamine
-
ir
Leu-2-naphthylamide + H2O
-
Rattus norvegicus Leu + 2-naphthylamine
-
ir
additional information activity as endopeptidase and aminopeptidase on synthetic substrates Homo sapiens ?
-
?
additional information activity as endopeptidase and aminopeptidase on synthetic substrates Rattus norvegicus ?
-
?
Proteins + H2O aminopeptidase and endopeptidase activity Homo sapiens ?
-
?
Proteins + H2O aminopeptidase and endopeptidase activity Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Homo sapiens
monomer
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Homo sapiens
40
-
assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.82
-
Arg-2-naphthylamide
-
Homo sapiens
1.82
-
benzoyl-Arg-2-naphthylamide
-
Homo sapiens
2.53
-
Arg-4-methylcoumaryl-7-amide
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 substrate azocasein Homo sapiens
5 6 substrate azocasein Rattus norvegicus
6.8
-
-
Rattus norvegicus
6.8
-
synthetic substrates Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
irreversible inactivation above Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
leupeptin
-
Rattus norvegicus