3.4.21.77: semenogelase
This is an abbreviated version!
For detailed information about semenogelase, go to the full flat file.
Word Map on EC 3.4.21.77
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3.4.21.77
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gleason
-
prostatectomy
-
androgen
-
rectal
-
radiotherapy
-
preoperative
-
hyperplasia
-
transrectal
-
urinary
-
metastases
-
adenocarcinoma
-
lymph
-
node
-
castration-resistant
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testosterone
-
postoperative
-
ultrasound
-
oncology
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univariate
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high-risk
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urological
-
pelvic
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beam
-
surveillance
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progression-free
-
staging
-
high-grade
-
lncap
-
low-risk
-
docetaxel
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nadir
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cancer-specific
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brachytherapy
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transurethral
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androgen-independent
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ultrasound-guided
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multiparametric
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neoadjuvant
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intermediate-risk
-
nomogram
-
suspicious
-
urologist
-
mcrpc
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antiandrogens
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ultrasonography
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lymphadenectomy
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erectile
-
incontinence
-
actuarial
-
cause-specific
-
analysis
-
medicine
-
diagnostics
-
drug development
- 3.4.21.77
-
gleason
-
prostatectomy
- androgen
- rectal
-
radiotherapy
-
preoperative
- hyperplasia
-
transrectal
- urinary
- metastases
- adenocarcinoma
- lymph
- node
-
castration-resistant
- testosterone
-
postoperative
-
ultrasound
-
oncology
-
univariate
-
high-risk
- urological
-
pelvic
-
beam
-
surveillance
-
progression-free
-
staging
-
high-grade
-
lncap
-
low-risk
- docetaxel
-
nadir
-
cancer-specific
-
brachytherapy
-
transurethral
-
androgen-independent
-
ultrasound-guided
-
multiparametric
-
neoadjuvant
-
intermediate-risk
-
nomogram
-
suspicious
-
urologist
-
mcrpc
-
antiandrogens
-
ultrasonography
-
lymphadenectomy
-
erectile
- incontinence
-
actuarial
-
cause-specific
- analysis
- medicine
- diagnostics
- drug development
Reaction
Preferential cleavage: -Tyr-/- =
Synonyms
7S nerve growth factor gamma chain, antigen (human clone lambdaHPSA-1 prostate-specific protein moiety reduced), antigen PSA (human clone 5P1 protein moiety reduced), antigen PSA (human prostate-specific), gama-SM protein, gamma-NGF, gamma-seminoglycoprotein (human protein moiety reduced), gamma-seminoprotein, gamma-SM, GK3, glandular kallikrein, glandular kallikrein 3, hK3/PSA, K3, K4, kallikrein 3, kallikrein-related peptidase 3, kallikrein-related peptidase-3, KLK3, P-30 antigen, prostate specific antigen, prostate stem cell antigen, prostate-specific antigen, prostate-specific antigen kallikrein, Prostate-specific membrane antigen, PSA, PSA-SV5, PSCA, PSMA, S01.162, seminal proteinase, seminin, six-transmembrane epithelial antigen of the prostate, STEAP, tissue kallikrein
ECTree
3.4.21.77 semenogelaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.4.21.77 - semenogelase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-morpholinecarbonyl-HSSKLQ-7-amido-4-methylcoumarin + H2O
?
a fluorogenic enzyme substrate
-
-
?
4-morpholinecarbonyl-SKLQ-7-amido-4-methylcoumarin + H2O
?
-
hydrolysis rate is 29.6 pmol/min per 100 pmol of PSA
-
-
?
EHSSKLQ-7-amido-4-methylcoumarin + H2O
EHSSKLQ + 7-amino-4-methylcoumarin
-
-
-
-
?
epsilon-maleimidocaproyl-Arg-Ser-Ser-Tyr-Tyr-Ser-Leu-p-aminobenzyloxycarbonyl-paclitaxel + H2O
epsilon-maleimidocaproyl-Arg-Ser-Ser-Tyr-Tyr + Ser-Leu-p-aminobenzyloxycarbonyl-paclitaxel
-
water soluble paclitaxel prodrug that is activated specifically by PSA in prostate tissue and prostate carcinoma
-
-
?
HSSKLQ-7-amido-4-methylcoumarin + H2O
?
-
hydrolysis rate is 62.7 pmol/min per 100 pmol of PSA
-
-
?
HSSKLQ-7-amido-4-methylcoumarin + H2O
HSSKLQ + 7-amino-4-methylcoumarin
-
-
-
-
?
insulin-like growth factor binding protein 5 + H2O
?
-
is degraded by PSA in a dose- and time-dependent manner. Under nonreducing conditions is degraded into two fragments with approximate molecular masses of 20 and 15 kDa. Under reducing conditions, is degraded into 4 distinct fragments with approximate molecular masses of 22 kDa, 21 kDa, 18 kDa and 13 kDa
-
-
?
insulin-like growth factor binding protein-3 + H2O
insulin-like growth factor I + ?
-
i.e. IGF-I
?
insulin-like growth factor binding protein-3 + H2O
insulin-like growth factor-1
-
-
-
-
?
KGISSQY-7-amido-4-methylcoumarin + H2O
KGISSQY + 7-amino-4-methylcoumarin
-
fluorogenic substrate
-
-
?
methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O
methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
morpholinocarbonyl-His-Ser-Ser-Lys-Leu-Gln-7-amido-4-(trifluoromethyl)-coumarin + H2O
?
-
-
-
?
morpholinocarbonyl-Lys-Gly-Ile-Ser-Ser-Gln-Tyr-7-amido-4-(trifluoromethyl)-coumarin + H2O
?
-
-
-
-
?
morpholinocarbonyl-Ser-Arg-Lys-Gln-Gln-Tyr-7-amido-4-methylcoumarin + H2O
?
-
-
-
-
?
Mu-SRKSQQY-7-amido-4-methylcoumarin + H2O
Mu-SRKSQQY + 7-amino-4-methylcoumarin
-
-
-
?
N-alpha-benzoyl-DL-Arg 4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
trypsin-like activity
-
-
?
N-succinyl-(Ala)3-p-nitroanilide + H2O
?
-
prostate-specific antigen shows limited activity
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-Ala-p-nitroanilide + H2O
?
-
prostate-specific antigen shows limited activity
-
-
?
N-succinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O
?
-
prostate-specific antigen shows limited activity
-
-
?
N-succinyl-Gly-Pro-Lys-p-nitroanilide + H2O
?
-
prostate-specific antigen activity is 3times higher towards this substrate than towards other synthetic substrates
-
-
?
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe 4-nitroanilide + H2O
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
chymotrypsin-like activity
-
-
?
o-aminobenzoyl-ISYQSSSTEEQ ethylene diamine 2,4-dinitrophenyl + 2 H2O
o-aminobenzoyl-ISYQ + SSST + EEQ ethylene diamine 2,4-dinitrophenyl
o-aminobenzoyl-ISYQSSSTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-ISY + QSSSTEEQ ethylene diamine 2,4-dinitrophenyl
o-aminobenzoyl-NKISYQSSSQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-NKISY + Q + SSSQ ethylene diamine 2,4-dinitrophenyl
o-aminobenzoyl-SSIYSNTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-SSIY + SNTEEQ ethylene diamine 2,4-dinitrophenyl
o-aminobenzoyl-SSQYSNTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-SSQY + SNTEEQ ethylene diamine 2,4-dinitrophenyl
PFR-7-amido-4-methylcoumarin + H2O
PFR + 7-amino-4-methylcoumarin
-
fluorogenic substrate
-
-
?
proform of transforming growth factor-beta + H2O
transforming growth factor-beta + ?
-
i.e. IGF-beta
?
succinyl-AAPF-7-amido-4-methylcoumarin + H2O
succinyl-AAPF + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
urokinase-type plasminogen activator receptor + H2O
?
-
-
cleavage within D1-D2 linker sequence and in its D3 juxtamembrane domain
-
?
Fibronectin + H2O
?
-
enzyme participates in sperm liquefaction by cleaving fibronectin and semenogelins, the major components of the seminal vesicle coagulum after ejaculation
-
-
?
Galectin-3 + H2O
?
-
carbohydrate-binding protein involved in cell adhesion, cell cycle control, immunomodulation, and cancer progression, including prostate cancer
PSA cleaves galactin-3 between residues Y107 and G108 to produce an active, monovalent lectin
-
?
Galectin-3 + H2O
?
galectin-3 Tyr-107 is phosphorylated by c-Abl. It can be cleaved at this site by the enzyme after Tyr107, resulting in loss of galectin-3 multivalency while preserving its carbohydrate binding activity
-
-
?
methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O
methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
-
chromogenic substrate
-
-
?
methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O
methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
-
i.e. S2586
-
-
?
o-aminobenzoyl-ISYQSSSTEEQ ethylene diamine 2,4-dinitrophenyl + 2 H2O
o-aminobenzoyl-ISYQ + SSST + EEQ ethylene diamine 2,4-dinitrophenyl
-
substrate is intramolecularly quenched
-
?
o-aminobenzoyl-ISYQSSSTEEQ ethylene diamine 2,4-dinitrophenyl + 2 H2O
o-aminobenzoyl-ISYQ + SSST + EEQ ethylene diamine 2,4-dinitrophenyl
-
synthetic fluorogenic substrate, that encompasses a sequence containing a major ex vivo cleavage site , the Gln266-Ser267 bond of semenogelin I
-
?
o-aminobenzoyl-ISYQSSSTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-ISY + QSSSTEEQ ethylene diamine 2,4-dinitrophenyl
-
substrate is intramolecularly quenched
-
?
o-aminobenzoyl-ISYQSSSTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-ISY + QSSSTEEQ ethylene diamine 2,4-dinitrophenyl
-
synthetic fluorogenic substrate
-
?
o-aminobenzoyl-NKISYQSSSQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-NKISY + Q + SSSQ ethylene diamine 2,4-dinitrophenyl
-
substrate is intramolecularly quenched
-
?
o-aminobenzoyl-NKISYQSSSQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-NKISY + Q + SSSQ ethylene diamine 2,4-dinitrophenyl
-
synthetic fluorogenic substrate
-
?
o-aminobenzoyl-SSIYSNTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-SSIY + SNTEEQ ethylene diamine 2,4-dinitrophenyl
-
substrate is intramolecularly quenched
-
?
o-aminobenzoyl-SSIYSNTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-SSIY + SNTEEQ ethylene diamine 2,4-dinitrophenyl
-
synthetic fluorogenic substrate
-
?
o-aminobenzoyl-SSIYSNTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-SSIY + SNTEEQ ethylene diamine 2,4-dinitrophenyl
-
sequence contains ex vivo cleavage sites of semenogelin I
-
?
o-aminobenzoyl-SSQYSNTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-SSQY + SNTEEQ ethylene diamine 2,4-dinitrophenyl
-
substrate is intramolecularly quenched
-
?
o-aminobenzoyl-SSQYSNTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-SSQY + SNTEEQ ethylene diamine 2,4-dinitrophenyl
-
synthetic fluorogenic substrate
-
?
o-aminobenzoyl-SSQYSNTEEQ ethylene diamine 2,4-dinitrophenyl + H2O
o-aminobenzoyl-SSQY + SNTEEQ ethylene diamine 2,4-dinitrophenyl
-
sequence contains ex vivo cleavage sites of semenogelin I
-
?
polypeptide + H2O
peptides
-
enzyme possibly is involved in the processing of insulin-like growth factor binding protein 3
-
?
polypeptide + H2O
peptides
enzyme possibly is involved in the processing of insulin-like growth factor binding protein 3
-
?
semenogelin + H2O
semenogelin fractions
-
enzyme participates in sperm liquefaction by cleaving fibronectin and semenogelins, the major components of the seminal vesicle coagulum after ejaculation
-
-
?
semenogelin + H2O
semenogelin fractions
-
semenogelins I and II, cleavage results in liquefaction and release of motil spermatozoa
-
?
semenogelin + H2O
semenogelin fragments
-
semenogelin I contains 18 cleavage sites, semenogelin II contains 16 cleavage sites
-
?
additional information
?
-
-
no activity with carbobenzoxylysine thiobenzyl ester
-
-
?
additional information
?
-
enzyme is regulated by steroid hormones, it contains 2 androgen-response elements in the proximal PSA promotor
-
-
?
additional information
?
-
-
enzyme is regulated by steroid hormones, it contains 2 androgen-response elements in the proximal PSA promotor
-
-
?
additional information
?
-
stimulates cell detachment and facilitates tumor spread
-
-
?
additional information
?
-
-
stimulates cell detachment and facilitates tumor spread
-
-
?
additional information
?
-
-
enzyme can be activated by recombinant kallikrein 2
-
-
?
additional information
?
-
-
slightly prefers Met over Nle and Ala at the P1 position of the fluorogenic substrates but also tolerates Leu, Tyr, Phe, and the basic residues Arg and Lys, whereas Asp and Thr are not accepted at all, In the S2 subsite Leu is slightly preferred over Ala, Gln, Met, and Tyr
-
-
?
additional information
?
-
-
proteolytic activity of prostate-specific antigen is essential for inhibition of angiogenesis
-
-
?
additional information
?
-
-
PSMA, PSCA and STEAP are specifically upregulated in the transgenic murine prostate cancer. Significant numbers of STEAP-specific CD8 T cells in the peripheral blood and the spleen of immune mice using MHC I tetramers
-
-
?
additional information
?
-
-
no hydrolysis with NH2-Q-amido-4-methyl coumarin, 4-morpholinecarbonyl-LQ-7-amido-4-methylcoumarin and 4-morpholinecarbonyl-KLQ-7-amido-4-methylcoumarin as substrates
-
-
?
additional information
?
-
-
substrates containing tyrosine in the P1 position are hydrolyzed efficiently by PSA. Substrates with glutamine in the P1 position are less efficiently hydrolyzed, but demonstrate better specificity for PSA. The region spanning the S1 pocket contains several highly conserved residues that are critical for the structural integrity of the pocket and indirectly responsible for the maintenance of enzymatic activity. Residues such as Cys191, Cys220, Pro225, and Ser214 form crucial elements of the architecture surrounding the S1 pocket
-
-
?
additional information
?
-
-
only P1-4 residues are major determinants of binding affinity and substrate specificity for PSA. The P1 residue binds at the specificity pocket lined by polar residues with the partial hydrophobic side-chains such as Ser217, Thr184, and Tyr219. The P2 residue side-chain is docked against the face of the imidazole ring of the catalytic His41 residue. The P3 residue acts as a lid to the specificity pocket and docks at a location just above the P1 binding site in the specificity pocket. Important role for Glu208 interactions in determining PSA specificity at the P3 residue. The P4 pocket is located in the lower groove area, and mainly formed by His164, Pro165, Gln166, and Trp205 residues
-
-
?
