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3.4.21.77: semenogelase

This is an abbreviated version!
For detailed information about semenogelase, go to the full flat file.

Word Map on EC 3.4.21.77

Reaction

Preferential cleavage: -Tyr-/- =

Synonyms

7S nerve growth factor gamma chain, antigen (human clone lambdaHPSA-1 prostate-specific protein moiety reduced), antigen PSA (human clone 5P1 protein moiety reduced), antigen PSA (human prostate-specific), gama-SM protein, gamma-NGF, gamma-seminoglycoprotein (human protein moiety reduced), gamma-seminoprotein, gamma-SM, GK3, glandular kallikrein, glandular kallikrein 3, hK3/PSA, K3, K4, kallikrein 3, kallikrein-related peptidase 3, kallikrein-related peptidase-3, KLK3, P-30 antigen, prostate specific antigen, prostate stem cell antigen, prostate-specific antigen, prostate-specific antigen kallikrein, Prostate-specific membrane antigen, PSA, PSA-SV5, PSCA, PSMA, S01.162, seminal proteinase, seminin, six-transmembrane epithelial antigen of the prostate, STEAP, tissue kallikrein

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.77 semenogelase

Purification

Purification on EC 3.4.21.77 - semenogelase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by centrifugation and gel filtration
-
by centrifugation, fractional precipitation by ammonium sulphate, ion-exchange chromatography and gel filtration, purified to homogeneity
-
by gel filtration
by HPLC
-
by lysozyme, sonication, and centrifugation, His-tagged PSA solublized from inclusion bodies by urea and purified on nickel-chelate resin. GST-fused PSA solublized from inclusion bodies by sarcosyl and purified on glutathione agarose beads
by thiophilic absorption chromatography
-
fast purification method with 90% of purity and 50% of recovery
-
free and complexed PSA, by gel filtration and immunoadsorption
-
from LNCaP cell culture medium
-
from seminal plasma
-
native active enzyme from seminal plasma by immunoaffinity chromatography and anion-exchange chromatography
nickel-nitrilotriacetic acid-Sepharose column chromatography and benzamidine-Sepharose column chromatography
-
one third of the purified protein is enzymatically inactive, due to carboxy-terminal cleavage of Lys145
-