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Literature summary for 3.4.21.77 extracted from

  • Debela, M.; Magdolen, V.; Schechter, N.; Valachova, M.; Lottspeich, F.; Craik, C.S.; Choe, Y.; Bode, W.; Goettig, P.
    Specificity profiling of seven human tissue kallikreins reveals individual subsite preferences (2006), J. Biol. Chem., 281, 25678-25688.
    View publication on PubMed
Search for more literature for 3.4.21.77

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15(pREP4) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin efficient inhibition Homo sapiens
Bovine pancreatic trypsin inhibitor efficient inhibition Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid-Sepharose column chromatography and benzamidine-Sepharose column chromatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate gland
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information slightly prefers Met over Nle and Ala at the P1 position of the fluorogenic substrates but also tolerates Leu, Tyr, Phe, and the basic residues Arg and Lys, whereas Asp and Thr are not accepted at all, In the S2 subsite Leu is slightly preferred over Ala, Gln, Met, and Tyr Homo sapiens ?
-
 ?
succinyl-AAPF-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens succinyl-AAPF + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
hK3/PSA exhibits a chymotrypsin-like specificity preferring large hydrophobic or polar residues at the P1 position Homo sapiens
prostate-specific antigen
-
 Homo sapiens
PSA
-
 Homo sapiens