Literature summary for 3.4.21.77 extracted from

Jeong, S.; Lee, S.W.
Expression and purification of recombinant active prostate-specific antigen from Escherichia coli (2007), J. Microbiol. Biotechnol., 17, 840-846.

Search for more literature for 3.4.21.77

Application

Application	Comment	Organism
medicine	purified active PSA has a potential use in prostate cancer diagnostics and/or therapeutics	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
proPSA and active PSA cDNA cleaved with EcoRI and HindIII and cloned into vector pET28(a)+, expressed in Escherichia coli strain BL21(DE3). Recombinant vector cleaved with EcoRI and XhoI, inserted into vector pGEX4T-1 and expressed in Escherichia coli strain BL21(DE3)	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	1 * 55000, SDS-PAGE, proPSA, 1 * 54000, SDS-PAGE, active PSA	Homo sapiens
55000	-	1 * 55000, SDS-PAGE, proPSA, 1 * 54000, SDS-PAGE, active PSA	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P07288	-	-

Purification (Commentary)

Purification (Comment)	Organism
by lysozyme, sonication, and centrifugation, His-tagged PSA solublized from inclusion bodies by urea and purified on nickel-chelate resin. GST-fused PSA solublized from inclusion bodies by sarcosyl and purified on glutathione agarose beads	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
LNCaP cell	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.004	-	purified recombinant active PSA	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
morpholinocarbonyl-His-Ser-Ser-Lys-Leu-Gln-7-amido-4-(trifluoromethyl)-coumarin + H2O	-	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 55000, SDS-PAGE, proPSA, 1 * 54000, SDS-PAGE, active PSA	Homo sapiens

Synonyms

Synonyms	Comment	Organism
prostate-specific antigen	-	Homo sapiens
PSA	-	Homo sapiens

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Release 2023.1 (January 2023)