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Literature summary for 3.4.21.77 extracted from

  • Menez, R.; Michel, S.; Muller, B.H.; Bossus, M.; Ducancel, F.; Jolivet-Reynaud, C.; Stura, E.A.
    Crystal structure of a ternary complex between human prostate-specific antigen, its substrate acyl intermediate and an activating antibody (2008), J. Mol. Biol., 376, 1021-1033.
    View publication on PubMed
Search for more literature for 3.4.21.77

Activating Compound

Activating Compound Comment Organism Structure
additional information monoclonal antibody 8G8F5 enhances enzymatic activity. It recognizes an epitope composed of five discontinuous segments including residues from the kallikrein loop and stabilizes PSA in an open and active conformation that accelerates catalysis Homo sapiens

Application

Application Comment Organism
medicine important marker for the diagnosis and management of prostate cancer Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
PSA in complex with monoclonal antibody 8G8F5 or PSA in complex with both 8G8F5 and the fluorogenic substrate morpholinocarbonyl-Lys-Gly-Ile-Ser-Ser-Gln-Tyr-7-amido-4-(trifluoromethyl)-coumarin, by sitting-drop vapor diffusion, between 2.83-3.33 A resolution. Belongs to space group P41212 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ at pH 7.5, 50% of the PSA enzymatic activity is inhibited by 0.024 mM, 10 mM are needed at pH 5.5 for inhibition. Inhibition is not relieved by monoclonal antibody binding of 8G8F5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by HPLC Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
semen
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
morpholinocarbonyl-Lys-Gly-Ile-Ser-Ser-Gln-Tyr-7-amido-4-(trifluoromethyl)-coumarin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
KLK3
-
 Homo sapiens
prostate-specific antigen
-
 Homo sapiens
PSA
-
 Homo sapiens