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Literature summary for 3.4.21.77 extracted from

  • Väisanen, V.; Lovgren, J.; Hellman, J.; Piironen, T.; Lilja, H.; Pettersson, K.
    Characterization and processing of prostate specific antigen (hK3) and human glandular kallikrein (hK2) secreted by LNCaP cells (1999), Prostate Cancer Prostatic. Dis., 2, 91-97.
    View publication on PubMed
Search for more literature for 3.4.21.77

Activating Compound

Activating Compound Comment Organism Structure
additional information inactive proPSA enzyme can be activated by recombinant kallikrein 2 to the mature form Homo sapiens

Application

Application Comment Organism
medicine the size of the fraction of free enzyme in serum is a marker to distinguish benign hyperplasia from malignant carcinoma Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
serum inhibits the mature enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28110 30000 inactive zymogen peak 2, gel filtration, SDS-PAGE and mass spectrometry Homo sapiens
28880 30000 active mature enzyme, gel filtration and mass spectrometry Homo sapiens
87000
-
 inactive zymogen peak 1, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme can be activated by recombinant kallikrein 2 ?
-
 ?
polypeptide + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 2 isozyme of the zymogen with different pI, several isoforms with different pIs of the mature enzyme
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification enzyme can be activated by recombinant kallikrein 2 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
from LNCaP cell culture medium Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate
-
 Homo sapiens
-
prostate cancer cell very low content of free enzyme Homo sapiens
-
prostate cancer cell produces equal amounts of zymogen proPSA and one-chain mature enzyme form, no intermediate forms Homo sapiens
-
prostate cancer cell metastatic prostate adenocarcinoma cell line LNCaP Homo sapiens
-
serum enzyme is mainly complexed to alpha1-antichymotrypsin, i.e. ACT, a small part of free enzyme Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
KGISSQY-7-amido-4-methylcoumarin + H2O fluorogenic substrate Homo sapiens KGISSQY + 7-amino-4-methylcoumarin
-
 ?
additional information enzyme can be activated by recombinant kallikrein 2 Homo sapiens ?
-
 ?
PFR-7-amido-4-methylcoumarin + H2O fluorogenic substrate Homo sapiens PFR + 7-amino-4-methylcoumarin
-
 ?
polypeptide + H2O
-
 Homo sapiens peptides
-
 ?

Subunits

Subunits Comment Organism
More active mature enzyme consists of only 1 chain Homo sapiens

Synonyms

Synonyms Comment Organism
K3
-
 Homo sapiens
prostate specific antigen
-
 Homo sapiens
prostate-specific antigen
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens several isozymes of active mature enzyme
-
 6.4
Homo sapiens 2 enzyme proforms with different pI: pH 8.2 and pH 8.4
-
 8.2
Homo sapiens 2 enzyme proforms with different pI: pH 8.2 and pH 8.4
-
 8.4