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3.4.21.77: semenogelase

This is an abbreviated version!
For detailed information about semenogelase, go to the full flat file.

Word Map on EC 3.4.21.77

Reaction

Preferential cleavage: -Tyr-/- =

Synonyms

7S nerve growth factor gamma chain, antigen (human clone lambdaHPSA-1 prostate-specific protein moiety reduced), antigen PSA (human clone 5P1 protein moiety reduced), antigen PSA (human prostate-specific), gama-SM protein, gamma-NGF, gamma-seminoglycoprotein (human protein moiety reduced), gamma-seminoprotein, gamma-SM, GK3, glandular kallikrein, glandular kallikrein 3, hK3/PSA, K3, K4, kallikrein 3, kallikrein-related peptidase 3, kallikrein-related peptidase-3, KLK3, P-30 antigen, prostate specific antigen, prostate stem cell antigen, prostate-specific antigen, prostate-specific antigen kallikrein, Prostate-specific membrane antigen, PSA, PSA-SV5, PSCA, PSMA, S01.162, seminal proteinase, seminin, six-transmembrane epithelial antigen of the prostate, STEAP, tissue kallikrein

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.77 semenogelase

Cloned

Cloned on EC 3.4.21.77 - semenogelase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli M15(pREP4) cells
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expressed in HUVEC cells
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gene hK3 or KLK3, genetic organization and structure of the kallikrein gene family, clustered on chromosome 19q13.3-q13.4
gene structure
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male C57BL/6 mice injected with recombinant adenoviruses containing rAd/PSMA, rAd/PSCA and rAd/STEAP
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proPSA and active PSA cDNA cleaved with EcoRI and HindIII and cloned into vector pET28(a)+, expressed in Escherichia coli strain BL21(DE3). Recombinant vector cleaved with EcoRI and XhoI, inserted into vector pGEX4T-1 and expressed in Escherichia coli strain BL21(DE3)
recombinant proteins of proPSA and active PSA cloned into pGEX4T-1 expression vector that produces recombinant proteins with N-terminal GST tags. The proteins expressed in Escherichia coli BL21(DE3)
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structure of the gene and linkage to the kallikrein-like gene hGK-1
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