3.4.21.5: thrombin

This is an abbreviated version, for detailed information about thrombin, go to the full flat file.

Reaction

selective cleavage of Arg-/-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B =

Synonyms

activated factor II, alpha-thrombin, alphaTh, beta-thrombin, blood-coagulation factor II, activated, blood-coagulation factor IIa, clotting factor IIa, EC 3.4.4.13, factor IIa, fibrinogenase, thrombase, thrombin, E, thrombin-C, thrombofort, topical, tropostasin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.5 thrombin

KM Value

KM Value on EC 3.4.21.5 - thrombin

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.181
Ac-Gly-Gly-Val-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 25C
0.102
Ac-Leu-Gly-Val-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 25C
0.067
Ac-Nle-Thr-Leu-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 25C
0.011
Ac-Nle-Thr-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 25C
0.03
Ac-Val-Thr-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 25C
0.0052 - 0.0079
benzoyl-Arg ethyl ester
0.0075 - 0.0088
benzoyl-Arg methyl ester
0.143 - 0.84
benzoyl-L-Arg-p-nitroanilide
0.0015 - 0.0632
D-Phe-L-pipecolyl-L-Arg-p-nitroanilide
-
0.00033 - 0.013
D-Phe-Pro-Arg-4-nitroanilide
0.004 - 0.072
D-Phe-Pro-Lys-4-nitroanilide
0.017 - 0.11
D-Phe-Pro-Phe-4-nitroanilide
0.0026 - 0.298
D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide
0.1471 - 0.2829
di-L-Glu-L-Pro-L-Arg-4-nitroanilide
0.112
factor VIII(341-376) peptide
-
pH 7.5, 25C
0.298
factor XIII
-
pH 7.4, 25C, wild-type enzyme
-
0.315
factor XIII V34L mutant
-
pH 7.4, 25C, wild-type enzyme
-
0.00001
Fc-[RFSRPQL]-PEG
-
-
0.00316 - 0.0528
fibrinogen
-
0.00355 - 0.0113
fibrinogen Aalpha chain
-
0.0051 - 0.013
Fibrinopeptide A
0.327
GLVPRGVNL
-
pH 7.4, 25C
0.386
GVVPRGVNL
-
pH 7.4, 25C
0.0084 - 0.028
PAR1 peptide
0.0355 - 0.181
platelet thrombin receptor peptide
-
0.002 - 0.0032
protein C
-
0.32
succinyl-AAPR-4-nitroanilide
-
-
0.000178
thrombin-activatable fibrinolysis inhibitor
-
presence of thrombomodulin, Km(app) value
-
0.0063 - 0.3
tosyl-Arg methyl ester
0.0085 - 0.0164
tosyl-Gly-Pro-Arg-4-nitroanilide
0.375
TVELQGLVPRGVNL
-
pH 7.4, 25C
0.644
TVELQGVVPRGVNL
-
pH 7.4, 25C
additional information
additional information
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