3.4.21.5: thrombin

This is an abbreviated version, for detailed information about thrombin, go to the full flat file.

Reaction

selective cleavage of Arg-/-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B =

Synonyms

activated factor II, alpha-thrombin, alphaTh, beta-thrombin, blood-coagulation factor II, activated, blood-coagulation factor IIa, clotting factor IIa, EC 3.4.4.13, factor IIa, fibrinogenase, thrombase, thrombin, E, thrombin-C, thrombofort, topical, tropostasin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.5 thrombin

Substrates Products

Substrates Products on EC 3.4.21.5 - thrombin

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ac-Gly-Gly-Val-Arg-7-amido-4-methylcoumarin + H2O
Ac-Gly-Gly-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Ac-Leu-Gly-Val-Arg-7-amido-4-methylcoumarin + H2O
Ac-Leu-Gly-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Ac-Nle-Thr-Leu-Arg-7-amido-4-methylcoumarin + H2O
Ac-Nle-Thr-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Ac-Nle-Thr-Pro-Arg-7-amido-4-methylcoumarin + H2O
Ac-Nle-Thr-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Ac-Val-Thr-Pro-Arg-7-amido-4-methylcoumarin + H2O
Ac-Val-Thr-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
activated protein C + H2O
?
show the reaction diagram
-
-
-
-
?
ADAMTS-13 + H2O
?
show the reaction diagram
Ala-Ala-Pro-Phe-4-nitroanilide + H2O
Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
synthetic chromogenic substrate
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-Arg + ethanol
show the reaction diagram
-
-
-
-
?
benzoyl-Arg methyl ester + H2O
benzoyl-Arg + methanol
show the reaction diagram
-
-
-
-
?
benzoyl-L-Arg-p-nitroanilide + H2O
benzoyl-L-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
beta-Ala-Gly-Arg-4-nitroanilide + H2O
beta-Ala-Gly-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
chromozym TH + H2O
? + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide + H2O
D-Phe-L-pipecolyl-L-Arg + 4-nitroanilide
show the reaction diagram
-
-
-
-
?
D-Phe-L-Pro-L-Arg-4-nitroanilide + H2O
D-Phe-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
D-Phe-L-Pro-L-Phe-4-nitroanilide + H2O
D-Phe-L-Pro-L-Phe + 4-nitroaniline
show the reaction diagram
D-Phe-Pip-Arg-4-nitroanilide + H2O
D-Phe-Pip-Arg + 4-nitroaniline
show the reaction diagram
-
i.e. S-2238
-
-
?
D-Phe-Pro-Arg-4-nitroanilide + H2O
D-Phe-Pro-Arg + 4-nitroaniline
show the reaction diagram
D-Phe-Pro-Lys-4-nitroanilide + H2O
D-Phe-Pro-Lys + 4-nitroaniline
show the reaction diagram
D-Phe-Pro-Phe-4-nitroanilide + H2O
D-Phe-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide + H2O
D-phenylalanyl-pipecolyl-L-arginine + 4-nitroaniline
show the reaction diagram
D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide + H2O
D-phenylalanyl-pipecolyl-L-arginine-4-nitroaniline
show the reaction diagram
-
-
-
-
?
di-L-Glu-L-Pro-L-Arg-4-nitroanilide + H2O
di-L-Glu-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
factor V (1018) + H2O
?
show the reaction diagram
-
cleavage site is LSPR
-
?
factor V (1545) + H2O
?
show the reaction diagram
-
cleavage site is WYLR
-
?
factor V (709) + H2O
?
show the reaction diagram
-
-
-
?
factor V + H2O
activated factor V + ?
show the reaction diagram
-
activation
-
-
?
factor V + H2O
factor Va + propeptide
show the reaction diagram
-
proteolytic activation
-
?
factor VII (1689) + H2O
?
show the reaction diagram
-
clevage site is QSPR
-
?
factor VII (372) + H2O
?
show the reaction diagram
-
cleavage site is IQIR
-
?
factor VII (740) + H2O
?
show the reaction diagram
-
-
-
?
factor VIII + H2O
?
show the reaction diagram
factor VIII + H2O
activated factor VIII + ?
show the reaction diagram
-
activation
-
-
?
factor VIII + H2O
factor VIIIa + propeptide
show the reaction diagram
factor VIII mutant D392A/D394A + H2O
?
show the reaction diagram
-
reduction in specific activity similar to a severe hemophilia phenotype. No cleavage at R740, while cleavage at R372 is not affected
-
-
?
factor VIII mutant Q370E/I371P/V374F/A375S + H2O
?
show the reaction diagram
-
-
mutation to P3-P3' residues flanking Arg740, 98% of the activtiy with wild-type
-
?
factor VIII mutant Q370S/I371P/V374F/A375Q + H2O
?
show the reaction diagram
-
-
mutation to P3-P3' residues flanking Arg1689, 14% of the activtiy with wild-type
-
?
factor VIII mutant R372H + H2O
?
show the reaction diagram
-
naturally occuring mutation in hemophilia A patients. About 80fold decrease in cleavage rate compared to wild-type substrate, cleavage at H372-S373 bond
-
-
?
factor VIII(341-376) peptide + H2O
?
show the reaction diagram
-
cleavage of Arg372 involving exosite II, the heparin binding site
-
?
factor X + H2O
factor Xa + propeptide
show the reaction diagram
factor XI + H2O
?
show the reaction diagram
-
-
-
?
factor XI + H2O
activated factor XI + ?
show the reaction diagram
-
activation
-
-
?
factor XI + H2O
factor XIa
show the reaction diagram
-
activation by thrombin
-
-
?
factor XI + H2O
factor XIa + propeptide
show the reaction diagram
-
proteolytic activation
-
?
factor XII + H2O
activated factor XII + ?
show the reaction diagram
factor XIII + H2O
?
show the reaction diagram
-
-
-
?
factor XIII + H2O
activated factor XIII + ?
show the reaction diagram
factor XIII + H2O
factor XIIIa + propeptide
show the reaction diagram
factor XIII V34L mutant + H2O
activated factor XIII V34L mutant + ?
show the reaction diagram
-
binding structure and interaction analysis, mutant substrate, a polymorphism exists within the activation peptide segment at the P4 position of FXIII resulting in substitution V34L, FXIII V34L occurs in approximately 30% of the human population worldwide, overview
-
-
?
Fc-[GRPS]-PEG + H2O
?
show the reaction diagram
-
ferrocene-labelled tetrapeptide with a polyethylene glycol linker
-
-
?
Fc-[RFSRPQL]-PEG + H2O
?
show the reaction diagram
-
ferrocene-labelled heptapeptide with a polyethylene glycol linker
-
-
?
fibrin I-plasma factor XIII complex + H2O
activation peptide + fibrinopeptide B
show the reaction diagram
-
-
-
?
Fibrinogen + H2O
?
show the reaction diagram
fibrinogen + H2O
fibrin
show the reaction diagram
-
-
-
-
?
fibrinogen + H2O
fibrin + ?
show the reaction diagram
fibrinogen + H2O
fibrin + fibrinoepeptide A + fibrinopeptide B
show the reaction diagram
-
-
-
-
?
fibrinogen + H2O
fibrin + fibrinopeptide A + fibrinopeptide B
show the reaction diagram
fibrinogen 1 + H2O
fibrin 1 + fibrinopeptide A + fibrinopeptide B
show the reaction diagram
fibrinogen 2 + H2O
fibrin 2 + fibrinopeptide A + fibrinopeptide B
show the reaction diagram
fibrinogen A a + H2O
?
show the reaction diagram
-
-
-
?
fibrinogen B b + H2O
?
show the reaction diagram
-
-
-
?
fibrinopeptide A + H2O
?
show the reaction diagram
galectin-8 + H2O
?
show the reaction diagram
galectin-9 + H2O
?
show the reaction diagram
GLVPRGVNL + H2O
GLVPR + GVNL
show the reaction diagram
-
residues 33-41 of factor XIII with mutation V34L
-
-
?
GVVPRGVNL + H2O
GVVPR + GVNL
show the reaction diagram
-
residues 33-41 of factor XIII
-
-
?
HD-cyclohexylglycyl-Ala-Arg-4-nitroanilide + H2O
HD-cyclohexylglycyl-Ala-Arg + 4-nitroaniline
show the reaction diagram
-
assay method optimization with synthetic substrate HD-cyclohexylglycyl-Ala-Arg-4-nitroanilide, overview
-
-
?
Meizothrombin + H2O
?
show the reaction diagram
-
cleavage of R155-S156 and R284-T285 bond
-
-
?
N-(4-tosyl)-Gly-L-Pro-L-Arg-4-nitroanilide + H2O
N-(4-tosyl)-Gly-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Arg 4-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Arg + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Lys 4-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-p'-(Nbeta,n-butyl-Nalpha-guanidino)benzoate + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-p'-(Nbeta,n-hexyl-Nalpha-guanidino)benzoate + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-p'-guanidinobenzoate + H2O
?
show the reaction diagram
-
-
-
-
?
PAR1 + H2O
?
show the reaction diagram
PAR1 peptide + H2O
?
show the reaction diagram
protease-activated receptor I peptide fragment, amino acid sequence
-
?
PAR3 + H2O
?
show the reaction diagram
-
protease-activated receptor 3
-
?
PAR4 + H2O
?
show the reaction diagram
Pefachrom tPa + H2O
?
show the reaction diagram
-
-
-
-
?
platelet thrombin receptor peptide + H2O
?
show the reaction diagram
-
-
-
-
?
prethrombin + H2O
?
show the reaction diagram
-
cleavage of R284-T285 bond
-
-
?
pro-factor XIII + H2O
factor XIII
show the reaction diagram
-
activation by cleavage at Arg37 leading to blood coagulation
-
?
profactor V + H2O
factor V
show the reaction diagram
-
human, activation, recombinant
-
?
profactor VIII + H2O
factor VIII
show the reaction diagram
-
human, activation, recombinant
-
?
protease-activated receptor + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor + H2O
activated protease-activated receptor + ?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor 1 + H2O
?
show the reaction diagram
protease-activated receptor 1 + H2O
activated protease-activated receptor 1 + ?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor 3 residues 44-56 + H2O
-
show the reaction diagram
-
-
-
-
?
protease-activated receptor 4 + H2O
activated PAR-4 + ?
show the reaction diagram
-
the cleaved form of protease-activated receptor 3, PAR-3, acts as a cofactor for thrombin cleavage and activation of PAR-4 on murine platelets, interaction analysis of thrombin with the extracellular part of PAR-4, overview
-
-
?
protease-activated receptor-1 + H2O
?
show the reaction diagram
protease-activated receptor-1 + H2O
activated PAR-1
show the reaction diagram
-
i.e. PAR-1, activation, major thrombin receptor
product induces connective tissue growth factor production, a fibroblast mitogen, which promotes extracellular matrix protein production
?
protease-activated receptor-3 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-4 + H2O
?
show the reaction diagram
-
activation
-
-
?
protein C + H2O
?
show the reaction diagram
protein C + H2O
activated protein C + ?
show the reaction diagram
-
solvent isotope effect study
-
-
?
protein C zymogen + H2O
activated protein C + propeptide
show the reaction diagram
protein kinase C + H2O
?
show the reaction diagram
-
activation
-
-
?
proteinase-activated receptor 1 + H2O
?
show the reaction diagram
-
alpha-thrombin may not effectively catalyze proteinase-activated receptor 1-(1-41) generation
-
-
?
proteinase-activated receptor 4 + H2O
?
show the reaction diagram
-
alpha-thrombin may not effectively catalyze proteinase-activated receptor 4-(1-47) generation
-
-
?
prothrombin + H2O
?
show the reaction diagram
-
cleavage of R155-S156 and R284-T285 bond
-
-
?
RAP + H2O
?
show the reaction diagram
-
-
-
-
?
S-thanatin + H2O
?
show the reaction diagram
-
-
-
-
?
S2238 + H2O
?
show the reaction diagram
spectrozyme TH + H2O
?
show the reaction diagram
-
-
-
-
?
spectrozyme-TH + H2O
?
show the reaction diagram
succinyl-AAPR-4-nitroanilide + H2O
succinyl-AAPR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
thrombin-activable finrinolysis inhibitor + H2O
?
show the reaction diagram
-
i.e. TAFI
-
?
thrombin-activatable fibrinolysis inhibitor + H2O
?
show the reaction diagram
Tosyl-Arg methyl ester + H2O
Tosyl-Arg + methanol
show the reaction diagram
tosyl-Gly-L-Pro-L-Arg-4-nitroanilide + H2O
tosyl-Gly-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
tosyl-Gly-Pro-Arg + 4-nitroaniline
show the reaction diagram
transmembrane receptor PAR1 + H2O
?
show the reaction diagram
-
-
-
?
TVELQGLVPRGVNL + H2O
TVELQGLVPR + GVNL
show the reaction diagram
-
residues 28-41 of factor XIII with mutation V34L
-
-
?
TVELQGVVPRGVNL + H2O
TVELQGVVPR + GVNL
show the reaction diagram
-
residues 28-41 of factor XIII
-
-
?
UpA + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-