3.4.21.5: thrombin

This is an abbreviated version, for detailed information about thrombin, go to the full flat file.

Reaction

selective cleavage of Arg-/-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B =

Synonyms

activated factor II, alpha-thrombin, alphaTh, beta-thrombin, blood-coagulation factor II, activated, blood-coagulation factor IIa, clotting factor IIa, EC 3.4.4.13, factor IIa, fibrinogenase, thrombase, thrombin, E, thrombin-C, thrombofort, topical, tropostasin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.5 thrombin

Purification

Purification on EC 3.4.21.5 - thrombin

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-thrombin and beta-thrombin
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dysprothrombin Perija
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from plasma, to homogeneity
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large-scale preparation from human plasma. Isolation of prothrombin is followed by activation to thrombin and further purification
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native enzyme from plasma
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partial
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recombinant enzyme mutants from BHK cells by anion and cation exchange chromatography to homogeneity
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recombinant protein
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recombinant wild-type and mutant enzyme from CHO cells
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recombinant wild-type and mutant enzymes to homogeneity
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recombinant wild-type enzyme and mutants from baby hamster kidney cells. Recombinant enzyme mutants W215M and W215T, and wild-type enzyme from Escherichia coli including unfolding and refolding of the pelleted enzymes, dialysis, heparin affinity hcromatography
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